Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks
The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft<sup>®</sup>, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE<sup>®</sup>, IBI S.A., Mezzovico-Vira, S...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/12/19/3234 |
| _version_ | 1811269053740744704 |
|---|---|
| author | Mike Barbeck Ole Jung Xin Xiong Rumen Krastev Tadas Korzinskas Stevo Najman Milena Radenković Nils Wegner Marina Knyazeva Frank Walther |
| author_facet | Mike Barbeck Ole Jung Xin Xiong Rumen Krastev Tadas Korzinskas Stevo Najman Milena Radenković Nils Wegner Marina Knyazeva Frank Walther |
| author_sort | Mike Barbeck |
| collection | DOAJ |
| description | The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft<sup>®</sup>, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE<sup>®</sup>, IBI S.A., Mezzovico-Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft<sup>®</sup> bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix. |
| first_indexed | 2024-04-12T21:34:02Z |
| format | Article |
| id | doaj.art-ecab89951309457781b8cb2d9a9e0a23 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-12T21:34:02Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ecab89951309457781b8cb2d9a9e0a232022-12-22T03:15:57ZengMDPI AGMaterials1996-19442019-10-011219323410.3390/ma12193234ma12193234Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone BlocksMike Barbeck0Ole Jung1Xin Xiong2Rumen Krastev3Tadas Korzinskas4Stevo Najman5Milena Radenković6Nils Wegner7Marina Knyazeva8Frank Walther9Department of Oral and Maxillofacial Surgery, Working Group Biomaterials/Surfaces, University Hospital Hamburg-Eppendorf, Hamburg 20246, Germany; ole.tiberius.jung@googlemail@com (O.J.)Department of Oral and Maxillofacial Surgery, Working Group Biomaterials/Surfaces, University Hospital Hamburg-Eppendorf, Hamburg 20246, Germany; ole.tiberius.jung@googlemail@com (O.J.)NMI, Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen 72770, GermanyFaculty of Applied Chemistry, Reutlingen University, Reutlingen 72770, GermanyDepartment of Oral and Maxillofacial Surgery, Working Group Biomaterials/Surfaces, University Hospital Hamburg-Eppendorf, Hamburg 20246, Germany; ole.tiberius.jung@googlemail@com (O.J.)Department for Cell and Tissue Engineering and Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, 18100 Niš, SerbiaDepartment for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Niš 18100, SerbiaDepartment of Materials Test Engineering (WPT), TU Dortmund University, Dortmund 44227, GermanyDepartment of Materials Test Engineering (WPT), TU Dortmund University, Dortmund 44227, GermanyDepartment of Materials Test Engineering (WPT), TU Dortmund University, Dortmund 44227, GermanyThe present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft<sup>®</sup>, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE<sup>®</sup>, IBI S.A., Mezzovico-Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft<sup>®</sup> bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix.https://www.mdpi.com/1996-1944/12/19/3234bone blockallogeneicxenogeneicpurificationbone regenerationdentistry |
| spellingShingle | Mike Barbeck Ole Jung Xin Xiong Rumen Krastev Tadas Korzinskas Stevo Najman Milena Radenković Nils Wegner Marina Knyazeva Frank Walther Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks Materials bone block allogeneic xenogeneic purification bone regeneration dentistry |
| title | Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks |
| title_full | Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks |
| title_fullStr | Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks |
| title_full_unstemmed | Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks |
| title_short | Balancing Purification and Ultrastructure of Naturally Derived Bone Blocks for Bone Regeneration: Report of the Purification Effort of Two Bone Blocks |
| title_sort | balancing purification and ultrastructure of naturally derived bone blocks for bone regeneration report of the purification effort of two bone blocks |
| topic | bone block allogeneic xenogeneic purification bone regeneration dentistry |
| url | https://www.mdpi.com/1996-1944/12/19/3234 |
| work_keys_str_mv | AT mikebarbeck balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT olejung balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT xinxiong balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT rumenkrastev balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT tadaskorzinskas balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT stevonajman balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT milenaradenkovic balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT nilswegner balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT marinaknyazeva balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks AT frankwalther balancingpurificationandultrastructureofnaturallyderivedboneblocksforboneregenerationreportofthepurificationeffortoftwoboneblocks |