Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels

Summary: This experiment was planned to investigate the efficiency of dietary crude protein (CP) levels (normal, medium [90% of the normal CP level] and low [80% of the normal CP level]) and betaine (Bet; 0 and 1 g/kg of diet) in broilers under heat stress (HS) conditions. A total of 672 one-day-old...

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Main Authors: H.A. Ghasemi, N. Nari
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Journal of Applied Poultry Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1056617119322408
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author H.A. Ghasemi
N. Nari
author_facet H.A. Ghasemi
N. Nari
author_sort H.A. Ghasemi
collection DOAJ
description Summary: This experiment was planned to investigate the efficiency of dietary crude protein (CP) levels (normal, medium [90% of the normal CP level] and low [80% of the normal CP level]) and betaine (Bet; 0 and 1 g/kg of diet) in broilers under heat stress (HS) conditions. A total of 672 one-day-old male broiler chickens were randomly assigned to 7 treatments (4 pens/treatment with 24 birds/pen). All experimental diets were supplemented with synthetic feed-grade lysine, methionine, and threonine at levels sufficient to meet dietary requirements. The positive control (PC) chickens were housed in a thermoneutral chamber and fed with a basal diet (normal diet without Bet supplementation). The other 6 groups were kept in a HS chamber (34°C) for 8 h (9:00–17:00). For the overall period of rearing (0–42 D), the HS control group (normal CP diet with no additive) exhibited lower (P < 0.001 or 0.05) average daily gain (ADG), European performance index (EPI), serum high-density lipoprotein cholesterol level, and primary antibody titers against Newcastle disease virus (NDV), but greater feed conversion ratio (FCR), mortality rate, serum glucose, cholesterol, low-density lipoprotein cholesterol levels, and heterophil to lymphocyte ratio (H/L) than those of the PC group. A decrease in dietary CP levels was accompanied by a decline in growth performance and the H/L ratio. Dietary Bet supplementation improved (P < 0.05) ADG, EPI, FCR, and primary antibody titers against NDV and infectious bronchitis virus in heat-stressed broilers, regardless of the CP levels in the diet. The two-way interaction effects of CP × Bet on all measured parameters were not significant. The present findings indicate that while reducing the CP content of the diet impairs broiler growth performance, dietary supplementation with Bet could not remove the unfavorable impacts of these kinds of diets. However, Bet improved growth performance and humoral immunity in heat-stressed broilers.
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spelling doaj.art-ecac7da063834f1faef8fd42b9a3977b2022-12-21T23:10:55ZengElsevierJournal of Applied Poultry Research1056-61712020-06-01292301313Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levelsH.A. Ghasemi0N. Nari1Corresponding author:; Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349 Arak, IranDepartment of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349 Arak, IranSummary: This experiment was planned to investigate the efficiency of dietary crude protein (CP) levels (normal, medium [90% of the normal CP level] and low [80% of the normal CP level]) and betaine (Bet; 0 and 1 g/kg of diet) in broilers under heat stress (HS) conditions. A total of 672 one-day-old male broiler chickens were randomly assigned to 7 treatments (4 pens/treatment with 24 birds/pen). All experimental diets were supplemented with synthetic feed-grade lysine, methionine, and threonine at levels sufficient to meet dietary requirements. The positive control (PC) chickens were housed in a thermoneutral chamber and fed with a basal diet (normal diet without Bet supplementation). The other 6 groups were kept in a HS chamber (34°C) for 8 h (9:00–17:00). For the overall period of rearing (0–42 D), the HS control group (normal CP diet with no additive) exhibited lower (P < 0.001 or 0.05) average daily gain (ADG), European performance index (EPI), serum high-density lipoprotein cholesterol level, and primary antibody titers against Newcastle disease virus (NDV), but greater feed conversion ratio (FCR), mortality rate, serum glucose, cholesterol, low-density lipoprotein cholesterol levels, and heterophil to lymphocyte ratio (H/L) than those of the PC group. A decrease in dietary CP levels was accompanied by a decline in growth performance and the H/L ratio. Dietary Bet supplementation improved (P < 0.05) ADG, EPI, FCR, and primary antibody titers against NDV and infectious bronchitis virus in heat-stressed broilers, regardless of the CP levels in the diet. The two-way interaction effects of CP × Bet on all measured parameters were not significant. The present findings indicate that while reducing the CP content of the diet impairs broiler growth performance, dietary supplementation with Bet could not remove the unfavorable impacts of these kinds of diets. However, Bet improved growth performance and humoral immunity in heat-stressed broilers.http://www.sciencedirect.com/science/article/pii/S1056617119322408dietary proteinbetainebroilersgrowth performanceimmunityheat stress
spellingShingle H.A. Ghasemi
N. Nari
Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
Journal of Applied Poultry Research
dietary protein
betaine
broilers
growth performance
immunity
heat stress
title Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
title_full Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
title_fullStr Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
title_full_unstemmed Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
title_short Effect of supplementary betaine on growth performance, blood biochemical profile, and immune response in heat-stressed broilers fed different dietary protein levels
title_sort effect of supplementary betaine on growth performance blood biochemical profile and immune response in heat stressed broilers fed different dietary protein levels
topic dietary protein
betaine
broilers
growth performance
immunity
heat stress
url http://www.sciencedirect.com/science/article/pii/S1056617119322408
work_keys_str_mv AT haghasemi effectofsupplementarybetaineongrowthperformancebloodbiochemicalprofileandimmuneresponseinheatstressedbroilersfeddifferentdietaryproteinlevels
AT nnari effectofsupplementarybetaineongrowthperformancebloodbiochemicalprofileandimmuneresponseinheatstressedbroilersfeddifferentdietaryproteinlevels