Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy
<p>To add new evidence that complement activation by the mannan (lectin) pathway is involved in the pathogenesis of nephropathy in patients with type 2 diabetes, we collected blood and urine samples from 20 patients with type 2 diabetes and nephropathy and 20 apparently healthy individuals fro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Slovenian Medical Association
2014-11-01
|
| Series: | Zdravniški Vestnik |
| Subjects: | |
| Online Access: | http://vestnik.szd.si/index.php/ZdravVest/article/view/103 |
| _version_ | 1818465657262440448 |
|---|---|
| author | Tjaša Cerar Draženka Pongrac Barlovič Anže Zorin Nadan Gregorič Vladimir Kotnik |
| author_facet | Tjaša Cerar Draženka Pongrac Barlovič Anže Zorin Nadan Gregorič Vladimir Kotnik |
| author_sort | Tjaša Cerar |
| collection | DOAJ |
| description | <p>To add new evidence that complement activation by the mannan (lectin) pathway is involved in the pathogenesis of nephropathy in patients with type 2 diabetes, we collected blood and urine samples from 20 patients with type 2 diabetes and nephropathy and 20 apparently healthy individuals from the general population. We performed tests for complement activation analysis (CH50, APH50, C3, C4, C3d/dg, C4d, SC5b-9, MBL and genotyping of <em>Mbl2</em> gene) in blood and (C3d/dg, C4d, MBL, and SC5b-9) in urine. We found significant signs of complement activation in patients with type 2 diabetes and nephropathy in blood and in urine. We also observed significant genetic differences between healthy individuals and patients with type 2 diabetes and nephropathy concerning the <em>Mbl2</em> gene. Healthy individuals were apparently more heterogeneous (45 % A0 type) in presentation of the structural <em>Mbl2</em> genotype, giving intermediate and low levels of MBL protein, compared to patients with type 2 diabetes and nephropathy, who had a very homogenous <em>Mbl2</em> genome (75 % AA type) giving a high level of MBL production. From our data we can conclude that patients with type 2 diabetes and nephropathy presents more frequently with <em>Mbl2</em> gene polymorphism giving high production of the MBL protein, what is very likely connected with acquired renal injury.</p> |
| first_indexed | 2024-04-13T20:48:33Z |
| format | Article |
| id | doaj.art-ecad86b01de14160b268f3aa3dd2d090 |
| institution | Directory Open Access Journal |
| issn | 1318-0347 1581-0224 |
| language | English |
| last_indexed | 2024-04-13T20:48:33Z |
| publishDate | 2014-11-01 |
| publisher | Slovenian Medical Association |
| record_format | Article |
| series | Zdravniški Vestnik |
| spelling | doaj.art-ecad86b01de14160b268f3aa3dd2d0902022-12-22T02:30:37ZengSlovenian Medical AssociationZdravniški Vestnik1318-03471581-02242014-11-018310934Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathyTjaša Cerar0Draženka Pongrac Barlovič1Anže Zorin2Nadan Gregorič3Vladimir Kotnik4Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, SloveniaClinical Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Center Ljubljana, Zaloška 7, 1000 Ljubljana, SloveniaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, SloveniaClinical Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Center Ljubljana, Zaloška 7, 1000 Ljubljana, SloveniaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia<p>To add new evidence that complement activation by the mannan (lectin) pathway is involved in the pathogenesis of nephropathy in patients with type 2 diabetes, we collected blood and urine samples from 20 patients with type 2 diabetes and nephropathy and 20 apparently healthy individuals from the general population. We performed tests for complement activation analysis (CH50, APH50, C3, C4, C3d/dg, C4d, SC5b-9, MBL and genotyping of <em>Mbl2</em> gene) in blood and (C3d/dg, C4d, MBL, and SC5b-9) in urine. We found significant signs of complement activation in patients with type 2 diabetes and nephropathy in blood and in urine. We also observed significant genetic differences between healthy individuals and patients with type 2 diabetes and nephropathy concerning the <em>Mbl2</em> gene. Healthy individuals were apparently more heterogeneous (45 % A0 type) in presentation of the structural <em>Mbl2</em> genotype, giving intermediate and low levels of MBL protein, compared to patients with type 2 diabetes and nephropathy, who had a very homogenous <em>Mbl2</em> genome (75 % AA type) giving a high level of MBL production. From our data we can conclude that patients with type 2 diabetes and nephropathy presents more frequently with <em>Mbl2</em> gene polymorphism giving high production of the MBL protein, what is very likely connected with acquired renal injury.</p>http://vestnik.szd.si/index.php/ZdravVest/article/view/103nephropathy, diabetes, complement, mannan binding lectin |
| spellingShingle | Tjaša Cerar Draženka Pongrac Barlovič Anže Zorin Nadan Gregorič Vladimir Kotnik Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy Zdravniški Vestnik nephropathy, diabetes, complement, mannan binding lectin |
| title | Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| title_full | Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| title_fullStr | Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| title_full_unstemmed | Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| title_short | Activation of complement system by mannan pathway and Mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| title_sort | activation of complement system by mannan pathway and mbl2 genotypes in patients with type 2 diabetes and nephropathy |
| topic | nephropathy, diabetes, complement, mannan binding lectin |
| url | http://vestnik.szd.si/index.php/ZdravVest/article/view/103 |
| work_keys_str_mv | AT tjasacerar activationofcomplementsystembymannanpathwayandmbl2genotypesinpatientswithtype2diabetesandnephropathy AT drazenkapongracbarlovic activationofcomplementsystembymannanpathwayandmbl2genotypesinpatientswithtype2diabetesandnephropathy AT anzezorin activationofcomplementsystembymannanpathwayandmbl2genotypesinpatientswithtype2diabetesandnephropathy AT nadangregoric activationofcomplementsystembymannanpathwayandmbl2genotypesinpatientswithtype2diabetesandnephropathy AT vladimirkotnik activationofcomplementsystembymannanpathwayandmbl2genotypesinpatientswithtype2diabetesandnephropathy |