Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method
Accurate focusing is a critical challenge of whole slide imaging, primarily due to inherent tissue topography variability. Traditional line scanning and tile-based scanning systems are limited in their ability to acquire a high degree of focus points while still maintaining high throughput. This rev...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2011;volume=2;issue=1;spage=44;epage=44;aulast=Montalto |
| _version_ | 1828337265884004352 |
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| author | Michael C Montalto Richard R McKay Robert J Filkins |
| author_facet | Michael C Montalto Richard R McKay Robert J Filkins |
| author_sort | Michael C Montalto |
| collection | DOAJ |
| description | Accurate focusing is a critical challenge of whole slide imaging, primarily due to inherent tissue topography variability. Traditional line scanning and tile-based scanning systems are limited in their ability to acquire a high degree of focus points while still maintaining high throughput. This review examines limitations with first-generation whole slide scanning systems and explores a novel approach that employs continuous autofocus, referred to as independent dual sensor scanning. This "second-generation" concept decouples image acquisition from focusing, allowing for rapid scanning while maintaining continuous accurate focus. The technical concepts, merits, and limitations of this method are explained and compared to that of a traditional whole slide scanning system. |
| first_indexed | 2024-04-13T22:13:15Z |
| format | Article |
| id | doaj.art-ecb091c8f0754216b984577900e2eec9 |
| institution | Directory Open Access Journal |
| issn | 2153-3539 2153-3539 |
| language | English |
| last_indexed | 2024-04-13T22:13:15Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-ecb091c8f0754216b984577900e2eec92022-12-22T02:27:39ZengElsevierJournal of Pathology Informatics2153-35392153-35392011-01-0121444410.4103/2153-3539.86282Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning methodMichael C MontaltoRichard R McKayRobert J FilkinsAccurate focusing is a critical challenge of whole slide imaging, primarily due to inherent tissue topography variability. Traditional line scanning and tile-based scanning systems are limited in their ability to acquire a high degree of focus points while still maintaining high throughput. This review examines limitations with first-generation whole slide scanning systems and explores a novel approach that employs continuous autofocus, referred to as independent dual sensor scanning. This "second-generation" concept decouples image acquisition from focusing, allowing for rapid scanning while maintaining continuous accurate focus. The technical concepts, merits, and limitations of this method are explained and compared to that of a traditional whole slide scanning system.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2011;volume=2;issue=1;spage=44;epage=44;aulast=MontaltoAutofocusdigital pathologywhole slide imaging |
| spellingShingle | Michael C Montalto Richard R McKay Robert J Filkins Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method Journal of Pathology Informatics Autofocus digital pathology whole slide imaging |
| title | Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method |
| title_full | Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method |
| title_fullStr | Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method |
| title_full_unstemmed | Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method |
| title_short | Autofocus methods of whole slide imaging systems and the introduction of a second-generation independent dual sensor scanning method |
| title_sort | autofocus methods of whole slide imaging systems and the introduction of a second generation independent dual sensor scanning method |
| topic | Autofocus digital pathology whole slide imaging |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2011;volume=2;issue=1;spage=44;epage=44;aulast=Montalto |
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