Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula
Mammary gland secretory cells produce miRNA-rich milk. In humans, these miRNAs reach infant/neonate bloodstream, playing diverse roles, like neural system development, metabolism, and immune system maturation. Notwithstanding, still few works explore human milk miRNA content, and there are no report...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2673-6284/11/2/11 |
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| author | Juan Manuel Vélez-Ixta Tizziani Benítez-Guerrero Arlene Aguilera-Hernández Helga Martínez-Corona Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Martín Noé Rangel-Calvillo Jaime García-Mena |
| author_facet | Juan Manuel Vélez-Ixta Tizziani Benítez-Guerrero Arlene Aguilera-Hernández Helga Martínez-Corona Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Martín Noé Rangel-Calvillo Jaime García-Mena |
| author_sort | Juan Manuel Vélez-Ixta |
| collection | DOAJ |
| description | Mammary gland secretory cells produce miRNA-rich milk. In humans, these miRNAs reach infant/neonate bloodstream, playing diverse roles, like neural system development, metabolism, and immune system maturation. Notwithstanding, still few works explore human milk miRNA content, and there are no reports at the population level. Our hypothesis was that miR-146b-5p, miR148a-3p, miR155-5p, mir181a-5p, and mir200a-3p immunoregulatory miRNAs are expressed in human colostrum/milk at a higher level than infant milk formulae. The aim of this work was to evaluate the expression of the five immunoregulatory miRNAs in human milk and compare it with their expression in infant milk formula. For this purpose, miRNA relative expression was measured by qPCR in cDNA prepared from total RNA extracted from sixty human colostrum/milk samples and six different formulae. The comparative Cт method 2<sup>−ΔCт</sup> using exogenous cel-miR-39 as internal control was employed, followed by statistical analysis. We found the relative expression levels of miRNAs are comparable among colostrum/milk samples, and these miRNAs are present in infant milk formulae but at very low concentrations. We conclude that the relative expression of the immunomodulatory miRNAs is comparable in all the human colostrum/milk samples and is higher than the expression in formulae. |
| first_indexed | 2024-03-10T00:17:27Z |
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| language | English |
| last_indexed | 2024-03-10T00:17:27Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
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| series | BioTech |
| spelling | doaj.art-ecb384f822ad4a3a8a1886b86f3cb7bd2023-11-23T15:49:33ZengMDPI AGBioTech2673-62842022-04-011121110.3390/biotech11020011Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk FormulaJuan Manuel Vélez-Ixta0Tizziani Benítez-Guerrero1Arlene Aguilera-Hernández2Helga Martínez-Corona3Karina Corona-Cervantes4Carmen Josefina Juárez-Castelán5Martín Noé Rangel-Calvillo6Jaime García-Mena7Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoHospital General “Dr. José María Rodríguez”, Instituto de Salud del Estado de Mexico, Ecatepec de Morelos 55200, Estado de México, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoMammary gland secretory cells produce miRNA-rich milk. In humans, these miRNAs reach infant/neonate bloodstream, playing diverse roles, like neural system development, metabolism, and immune system maturation. Notwithstanding, still few works explore human milk miRNA content, and there are no reports at the population level. Our hypothesis was that miR-146b-5p, miR148a-3p, miR155-5p, mir181a-5p, and mir200a-3p immunoregulatory miRNAs are expressed in human colostrum/milk at a higher level than infant milk formulae. The aim of this work was to evaluate the expression of the five immunoregulatory miRNAs in human milk and compare it with their expression in infant milk formula. For this purpose, miRNA relative expression was measured by qPCR in cDNA prepared from total RNA extracted from sixty human colostrum/milk samples and six different formulae. The comparative Cт method 2<sup>−ΔCт</sup> using exogenous cel-miR-39 as internal control was employed, followed by statistical analysis. We found the relative expression levels of miRNAs are comparable among colostrum/milk samples, and these miRNAs are present in infant milk formulae but at very low concentrations. We conclude that the relative expression of the immunomodulatory miRNAs is comparable in all the human colostrum/milk samples and is higher than the expression in formulae.https://www.mdpi.com/2673-6284/11/2/11human milkqPCRmicroRNARNAformula milkbreastfeeding |
| spellingShingle | Juan Manuel Vélez-Ixta Tizziani Benítez-Guerrero Arlene Aguilera-Hernández Helga Martínez-Corona Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Martín Noé Rangel-Calvillo Jaime García-Mena Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula BioTech human milk qPCR microRNA RNA formula milk breastfeeding |
| title | Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula |
| title_full | Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula |
| title_fullStr | Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula |
| title_full_unstemmed | Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula |
| title_short | Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula |
| title_sort | detection and quantification of immunoregulatory mirnas in human milk and infant milk formula |
| topic | human milk qPCR microRNA RNA formula milk breastfeeding |
| url | https://www.mdpi.com/2673-6284/11/2/11 |
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