Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.
<h4>Background and aims</h4>Subjects with congenital chloride diarrhea (CLD; a defect in solute carrier family 26 member 3 (SLC26A3)) are prone to inflammatory bowel disease (IBD). We investigated fecal microbiota in CLD and CLD-associated IBD. We also tested whether microbiota is modula...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0269561 |
| _version_ | 1811292635988492288 |
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| author | Satu Wedenoja Aki Saarikivi Jani Mälkönen Saara Leskinen Markku Lehto Krishna Adeshara Jetta Tuokkola Anne Nikkonen Laura Merras-Salmio Miikka Höyhtyä Sohvi Hörkkö Anu Haaramo Anne Salonen Willem M de Vos Katri Korpela Kaija-Leena Kolho |
| author_facet | Satu Wedenoja Aki Saarikivi Jani Mälkönen Saara Leskinen Markku Lehto Krishna Adeshara Jetta Tuokkola Anne Nikkonen Laura Merras-Salmio Miikka Höyhtyä Sohvi Hörkkö Anu Haaramo Anne Salonen Willem M de Vos Katri Korpela Kaija-Leena Kolho |
| author_sort | Satu Wedenoja |
| collection | DOAJ |
| description | <h4>Background and aims</h4>Subjects with congenital chloride diarrhea (CLD; a defect in solute carrier family 26 member 3 (SLC26A3)) are prone to inflammatory bowel disease (IBD). We investigated fecal microbiota in CLD and CLD-associated IBD. We also tested whether microbiota is modulated by supplementation with the short-chain fatty acid butyrate.<h4>Subjects and methods</h4>We recruited 30 patients with CLD for an observational 3-week follow-up study. Thereafter, 16 consented to oral butyrate substitution for a 3-week observational period. Fecal samples, collected once a week, were assayed for calprotectin and potential markers of inflammation, and studied by 16S ribosomal ribonucleic acid (rRNA) gene amplicon sequencing and compared to that of 19 healthy controls and 43 controls with Crohn's disease. Data on intestinal symptoms, diet and quality of life were collected.<h4>Results</h4>Patients with CLD had increased abundances of Proteobacteria, Veillonella, and Prevotella, and lower abundances of normally dominant taxa Ruminococcaceae and Lachnospiraceae when compared with healthy controls and Crohn´s disease. No major differences in fecal microbiota were found between CLD and CLD-associated IBD (including two with yet untreated IBD). Butyrate was poorly tolerated and showed no major effects on fecal microbiota or biomarkers in CLD.<h4>Conclusions</h4>Fecal microbiota in CLD is different from that of healthy subjects or Crohn´s disease. Unexpectedly, no changes in the microbiota or fecal markers characterized CLD-associated IBD, an entity with high frequency among patients with CLD. |
| first_indexed | 2024-04-13T04:48:20Z |
| format | Article |
| id | doaj.art-ecbd272b12df4bbbbf096ff751597a56 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T04:48:20Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ecbd272b12df4bbbbf096ff751597a562022-12-22T03:01:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176e026956110.1371/journal.pone.0269561Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.Satu WedenojaAki SaarikiviJani MälkönenSaara LeskinenMarkku LehtoKrishna AdesharaJetta TuokkolaAnne NikkonenLaura Merras-SalmioMiikka HöyhtyäSohvi HörkköAnu HaaramoAnne SalonenWillem M de VosKatri KorpelaKaija-Leena Kolho<h4>Background and aims</h4>Subjects with congenital chloride diarrhea (CLD; a defect in solute carrier family 26 member 3 (SLC26A3)) are prone to inflammatory bowel disease (IBD). We investigated fecal microbiota in CLD and CLD-associated IBD. We also tested whether microbiota is modulated by supplementation with the short-chain fatty acid butyrate.<h4>Subjects and methods</h4>We recruited 30 patients with CLD for an observational 3-week follow-up study. Thereafter, 16 consented to oral butyrate substitution for a 3-week observational period. Fecal samples, collected once a week, were assayed for calprotectin and potential markers of inflammation, and studied by 16S ribosomal ribonucleic acid (rRNA) gene amplicon sequencing and compared to that of 19 healthy controls and 43 controls with Crohn's disease. Data on intestinal symptoms, diet and quality of life were collected.<h4>Results</h4>Patients with CLD had increased abundances of Proteobacteria, Veillonella, and Prevotella, and lower abundances of normally dominant taxa Ruminococcaceae and Lachnospiraceae when compared with healthy controls and Crohn´s disease. No major differences in fecal microbiota were found between CLD and CLD-associated IBD (including two with yet untreated IBD). Butyrate was poorly tolerated and showed no major effects on fecal microbiota or biomarkers in CLD.<h4>Conclusions</h4>Fecal microbiota in CLD is different from that of healthy subjects or Crohn´s disease. Unexpectedly, no changes in the microbiota or fecal markers characterized CLD-associated IBD, an entity with high frequency among patients with CLD.https://doi.org/10.1371/journal.pone.0269561 |
| spellingShingle | Satu Wedenoja Aki Saarikivi Jani Mälkönen Saara Leskinen Markku Lehto Krishna Adeshara Jetta Tuokkola Anne Nikkonen Laura Merras-Salmio Miikka Höyhtyä Sohvi Hörkkö Anu Haaramo Anne Salonen Willem M de Vos Katri Korpela Kaija-Leena Kolho Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. PLoS ONE |
| title | Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. |
| title_full | Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. |
| title_fullStr | Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. |
| title_full_unstemmed | Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. |
| title_short | Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease. |
| title_sort | fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease |
| url | https://doi.org/10.1371/journal.pone.0269561 |
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