Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro
Shuai Shi, Xuechen Zhu, QingFa Guo, Yingjing Wang, Tao Zuo, Feng Luo, Zhiyong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of ChinaBackground: In this paper, a series of amphiphili...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2012-03-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/self-assembled-mpegndashpcl-gndashpei-micelles-for-simultaneous-codeli-a9609 |
| _version_ | 1818683597965492224 |
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| author | Shi S Zhu XC Guo QF Wang YJ Zuo T Luo F Qian ZY |
| author_facet | Shi S Zhu XC Guo QF Wang YJ Zuo T Luo F Qian ZY |
| author_sort | Shi S |
| collection | DOAJ |
| description | Shuai Shi, Xuechen Zhu, QingFa Guo, Yingjing Wang, Tao Zuo, Feng Luo, Zhiyong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of ChinaBackground: In this paper, a series of amphiphilic triblock copolymers based on polyethylene glycol–poly ε-caprolactone–polyethylenimine (mPEG–PCL-g–PEI) were successfully synthesized, and their application for codelivery of chemotherapeutic drugs and DNA simultaneously was investigated.Methods and results: These copolymers could self-assemble into micelles with positive charges. The size and zeta potential of the micelles was measured, and the results indicate that temperature had a large effect on the micelles obtained. In vitro gene transfection evaluation in cancer cells indicated that the self-assembled micelles could serve as potential gene delivery vectors. In addition, hydrophobic drug entrapment efficiency and codelivery with the gene was also studied in vitro. The self-assembled micelles could load doxorubicin efficiently and increase cellular uptake in vitro, while maintaining high gene transfection efficiency.Conclusion: The triblock copolymer mPEG–PCL-g–PEI could be a novel vector for codelivery of drug and gene therapy.Keywords: self-assembly, triblock copolymer, DNA, drug codelivery, gene transfection |
| first_indexed | 2024-12-17T10:37:17Z |
| format | Article |
| id | doaj.art-ecc5a34efcb54c46a10f084528a8651f |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T10:37:17Z |
| publishDate | 2012-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-ecc5a34efcb54c46a10f084528a8651f2022-12-21T21:52:21ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132012-03-012012default17491759Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitroShi SZhu XCGuo QFWang YJZuo TLuo FQian ZYShuai Shi, Xuechen Zhu, QingFa Guo, Yingjing Wang, Tao Zuo, Feng Luo, Zhiyong QianState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of ChinaBackground: In this paper, a series of amphiphilic triblock copolymers based on polyethylene glycol–poly ε-caprolactone–polyethylenimine (mPEG–PCL-g–PEI) were successfully synthesized, and their application for codelivery of chemotherapeutic drugs and DNA simultaneously was investigated.Methods and results: These copolymers could self-assemble into micelles with positive charges. The size and zeta potential of the micelles was measured, and the results indicate that temperature had a large effect on the micelles obtained. In vitro gene transfection evaluation in cancer cells indicated that the self-assembled micelles could serve as potential gene delivery vectors. In addition, hydrophobic drug entrapment efficiency and codelivery with the gene was also studied in vitro. The self-assembled micelles could load doxorubicin efficiently and increase cellular uptake in vitro, while maintaining high gene transfection efficiency.Conclusion: The triblock copolymer mPEG–PCL-g–PEI could be a novel vector for codelivery of drug and gene therapy.Keywords: self-assembly, triblock copolymer, DNA, drug codelivery, gene transfectionhttp://www.dovepress.com/self-assembled-mpegndashpcl-gndashpei-micelles-for-simultaneous-codeli-a9609 |
| spellingShingle | Shi S Zhu XC Guo QF Wang YJ Zuo T Luo F Qian ZY Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro International Journal of Nanomedicine |
| title | Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro |
| title_full | Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro |
| title_fullStr | Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro |
| title_full_unstemmed | Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro |
| title_short | Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro |
| title_sort | self assembled mpeg amp ndash pcl g amp ndash pei micelles for simultaneous codelivery of chemotherapeutic drugs and dna synthesis and characterization in vitro |
| url | http://www.dovepress.com/self-assembled-mpegndashpcl-gndashpei-micelles-for-simultaneous-codeli-a9609 |
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