Functional Conservation and Divergence of <i>MOS1</i> That Controls Flowering Time and Seed Size in Rice and <i>Arabidopsis</i>
The heading date and grain size are two essential traits affecting rice yield. Here, we found that <i>OsMOS1</i> promotes rice heading and affects its grain size. Knocking out <i>OsMOS1</i> delayed heading, while the overexpression of <i>OsMOS1</i> promoted headin...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/21/13448 |
| Summary: | The heading date and grain size are two essential traits affecting rice yield. Here, we found that <i>OsMOS1</i> promotes rice heading and affects its grain size. Knocking out <i>OsMOS1</i> delayed heading, while the overexpression of <i>OsMOS1</i> promoted heading in rice under long-day conditions. The transcriptions of the heading activators <i>Ehd1</i>, <i>Hd3a</i>, and <i>RFT1</i> were decreased and the heading repressor <i>Hd1</i> was increased in the <i>osmos1</i> mutant. Conversely, the overexpression of <i>OsMOS1</i> promoted the expressions of <i>Ehd1</i>, <i>Hd3a</i>, and <i>RFT1</i>, but inhibited the expression of <i>Hd1</i>. This suggests that <i>OsMOS1</i> may control heading in rice by modulating the transcriptions of <i>Ehd1</i>, <i>Hd3a</i>, <i>RFT1</i>, and <i>Hd1</i>. In addition, knocking out <i>OsMOS1</i> led to larger grains with longer grain lengths and higher grain weights. The seed cell size measurement showed that the cell lengths and cell widths of the outer glume epidermal cells of the <i>osmos1</i> mutant were greater than those of the wild type. Furthermore, we also found that the overexpression of <i>OsMOS1</i> in the <i>Arabidopsis mos1</i> mutant background could suppress its phenotypes of late flowering and increased seed size. Thus, our study shows a conserved function of <i>MOS1</i> in rice and <i>Arabidopsis</i>, and these findings shed light on the heading and seed size regulation in rice and suggest that <i>OsMOS1</i> is a promising target for rice yield improvement. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |