Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity
Abstract Toll‐like receptors (TLRs) are an important family of pattern‐recognition receptors that recognize a series of pathogen‐associated molecular patterns to trigger the host immune response. In the present study, we identified three TLR13 genes (i.e., LcLTR13a, LcTLR13b, and LcTLR13c) in Americ...
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| Format: | Article |
| Language: | English |
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Wiley
2024-04-01
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| Series: | Journal of the World Aquaculture Society |
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| Online Access: | https://doi.org/10.1111/jwas.13030 |
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| author | Peikui Yang Jude Juventus Aweya Lifan Guo Yalei Yang Xuelian Ye Hao Yang Yaqun Liu Yuzhong Zheng |
| author_facet | Peikui Yang Jude Juventus Aweya Lifan Guo Yalei Yang Xuelian Ye Hao Yang Yaqun Liu Yuzhong Zheng |
| author_sort | Peikui Yang |
| collection | DOAJ |
| description | Abstract Toll‐like receptors (TLRs) are an important family of pattern‐recognition receptors that recognize a series of pathogen‐associated molecular patterns to trigger the host immune response. In the present study, we identified three TLR13 genes (i.e., LcLTR13a, LcTLR13b, and LcTLR13c) in American bullfrogs, Lithobates catesbeianus, and explored their constitutive and pathogen‐induced expression profile in different tissues. The open reading frames of LcLTR13a, LcTLR13b and LcTLR13c are 2811, 2844, and 2814 bp long, encoding 955, 947, and 933 amino acids, respectively. Phylogenetic analysis showed that three LcTLR13 genes grouping in different subclades indicate the gene duplication of bullfrog TLR13 as in teleost. The three LcTLR13 genes were expressed in all the tissues examined, with the liver and spleen expressing the higher levels. Moreover, the expression levels of LcLTR13a, LcTLR13b, and LcTLR13c were significantly induced in the liver and spleen after bullfrogs were challenged with bacterial (i.e., Citrobacter freundii, Aeromonas hydrophila, and Edwardsiella tarda) pathogens. These results indicate that LcTLR13 genes play an essential role in the bullfrog antibacterial innate immune response, providing new insights into immune mechanisms of bullfrogs. |
| first_indexed | 2024-04-24T12:31:16Z |
| format | Article |
| id | doaj.art-ecc82e3126e74fe88997986ff422eeab |
| institution | Directory Open Access Journal |
| issn | 0893-8849 1749-7345 |
| language | English |
| last_indexed | 2024-04-24T12:31:16Z |
| publishDate | 2024-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of the World Aquaculture Society |
| spelling | doaj.art-ecc82e3126e74fe88997986ff422eeab2024-04-08T04:31:17ZengWileyJournal of the World Aquaculture Society0893-88491749-73452024-04-01552n/an/a10.1111/jwas.13030Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunityPeikui Yang0Jude Juventus Aweya1Lifan Guo2Yalei Yang3Xuelian Ye4Hao Yang5Yaqun Liu6Yuzhong Zheng7School of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Jimei University Xiamen Fujian ChinaSchool of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaSchool of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaSchool of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaChaoan Guihu Weixing Freshwater Aquaculture Co., Ltd Chaozhou Guangdong ChinaSchool of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaSchool of Life Science and Food Engineering Hanshan Normal University Chaozhou Guangdong ChinaAbstract Toll‐like receptors (TLRs) are an important family of pattern‐recognition receptors that recognize a series of pathogen‐associated molecular patterns to trigger the host immune response. In the present study, we identified three TLR13 genes (i.e., LcLTR13a, LcTLR13b, and LcTLR13c) in American bullfrogs, Lithobates catesbeianus, and explored their constitutive and pathogen‐induced expression profile in different tissues. The open reading frames of LcLTR13a, LcTLR13b and LcTLR13c are 2811, 2844, and 2814 bp long, encoding 955, 947, and 933 amino acids, respectively. Phylogenetic analysis showed that three LcTLR13 genes grouping in different subclades indicate the gene duplication of bullfrog TLR13 as in teleost. The three LcTLR13 genes were expressed in all the tissues examined, with the liver and spleen expressing the higher levels. Moreover, the expression levels of LcLTR13a, LcTLR13b, and LcTLR13c were significantly induced in the liver and spleen after bullfrogs were challenged with bacterial (i.e., Citrobacter freundii, Aeromonas hydrophila, and Edwardsiella tarda) pathogens. These results indicate that LcTLR13 genes play an essential role in the bullfrog antibacterial innate immune response, providing new insights into immune mechanisms of bullfrogs.https://doi.org/10.1111/jwas.13030bullfrogimmune stimulationinnate immuneLithobates catesbeianustoll‐like receptor 13 |
| spellingShingle | Peikui Yang Jude Juventus Aweya Lifan Guo Yalei Yang Xuelian Ye Hao Yang Yaqun Liu Yuzhong Zheng Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity Journal of the World Aquaculture Society bullfrog immune stimulation innate immune Lithobates catesbeianus toll‐like receptor 13 |
| title | Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity |
| title_full | Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity |
| title_fullStr | Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity |
| title_full_unstemmed | Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity |
| title_short | Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity |
| title_sort | characterization of the toll like receptor 13 homologs in american bullfrogs lithobates catesbeianus and their involvement in antimicrobial immunity |
| topic | bullfrog immune stimulation innate immune Lithobates catesbeianus toll‐like receptor 13 |
| url | https://doi.org/10.1111/jwas.13030 |
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