PBMC fixation and processing for Chromium single-cell RNA sequencing

PBMC fixation and processing for Chromium single-cell RNA sequencing

Abstract Background Interest in single-cell transcriptomic analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In almost all reported works investigators have used live cells, which introduces cell stress during preparation and hinders complex study desi...

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Bibliographic Details
Main Authors: Jinguo Chen, Foo Cheung, Rongye Shi, Huizhi Zhou, Wenrui Lu, CHI Consortium
Format: Article
Language:English
Published: BMC 2018-07-01
Series:Journal of Translational Medicine
Subjects:
Fixation
Methanol
SSC
PBMC
Primary cells
Droplet-based single-cell RNA-Seq
Online Access:http://link.springer.com/article/10.1186/s12967-018-1578-4

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