Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract
Abstract Background Natural products are an important source of bioproducts with pharmacological properties. Here we investigate the components of leaves from M. tomentosa Benth. (Fritsch) (Chrysobalanaceae) and its effects on bacterial cell growth, biofilm production and macrophage activity. Method...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-023-03968-z |
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| author | Mariana Freire Campos Leopoldo Clemente Baratto Vinícius Mendes Vidal Ivana Ventura Nascimento Brendo Araujo Gomes Genes de Lima Martins Neto Priscilla Christina Olsen Rodrigo Ribeiro Tarjano Leo Lilian Oliveira Moreira |
| author_facet | Mariana Freire Campos Leopoldo Clemente Baratto Vinícius Mendes Vidal Ivana Ventura Nascimento Brendo Araujo Gomes Genes de Lima Martins Neto Priscilla Christina Olsen Rodrigo Ribeiro Tarjano Leo Lilian Oliveira Moreira |
| author_sort | Mariana Freire Campos |
| collection | DOAJ |
| description | Abstract Background Natural products are an important source of bioproducts with pharmacological properties. Here we investigate the components of leaves from M. tomentosa Benth. (Fritsch) (Chrysobalanaceae) and its effects on bacterial cell growth, biofilm production and macrophage activity. Methods The effect of the different leaf extracts against bacterial cell growth was performed using the microdilution method. The most active extract was analyzed by mass spectrometry, and its effect on bacterial biofilm production was evaluated on polystyrene plates. The extract effect on macrophage activity was tested in the RAW264.7 cell line, which was stimulated with different concentrations of the extract in the presence or absence of LPS. Results We show that the ethyl acetate (EtOAc) extract was the most effective against bacterial cell growth. EtOAc extract DI-ESI (-)MSn analysis showed the presence of a glycosylated flavonoid tentatively assigned as myricetin 3-O-xylosyl-rhamnoside (MW 596). Also, the EtOAc extract increased biofilm formation by S. aureus and inhibited cytokine and NO production induced by LPS in RAW macrophages. Conclusion M. tomentosa flavonoid-enriched EtOAc extract presented a bactericidal and anti-inflammatory pharmacological potential. |
| first_indexed | 2024-03-12T23:26:39Z |
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| id | doaj.art-eccd1af48f744af4b72145d0b04e97bc |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-12T23:26:39Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-eccd1af48f744af4b72145d0b04e97bc2023-07-16T11:09:28ZengBMCBMC Complementary Medicine and Therapies2662-76712023-05-012311910.1186/s12906-023-03968-zBactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extractMariana Freire Campos0Leopoldo Clemente Baratto1Vinícius Mendes Vidal2Ivana Ventura Nascimento3Brendo Araujo Gomes4Genes de Lima Martins Neto5Priscilla Christina Olsen6Rodrigo Ribeiro Tarjano Leo7Lilian Oliveira Moreira8Laboratório de Anatomia Vegetal, Departamento de Botânica, Federal University of the State of Rio de Janeiro (UNRIO)Laboratório de Farmacognosia Aplicada, Departamento de Produtos Naturais e Alimentos, Federal University of Rio de Janeiro (UFRJ)Laboratório de Bacteriologia e Imunologia Clínica (LABIC), Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Federal University of Rio de Janeiro (UFRJ)Laboratório de Bacteriologia e Imunologia Clínica (LABIC), Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Federal University of Rio de Janeiro (UFRJ)Laboratório de Anatomia Vegetal, Departamento de Botânica, Federal University of the State of Rio de Janeiro (UNRIO)Laboratório de Anatomia Vegetal, Departamento de Botânica, Federal University of the State of Rio de Janeiro (UNRIO)Laboratório de Bacteriologia e Imunologia Clínica (LABIC), Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Federal University of Rio de Janeiro (UFRJ)Laboratório de Anatomia Vegetal, Departamento de Botânica, Federal University of the State of Rio de Janeiro (UNRIO)Laboratório de Bacteriologia e Imunologia Clínica (LABIC), Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Federal University of Rio de Janeiro (UFRJ)Abstract Background Natural products are an important source of bioproducts with pharmacological properties. Here we investigate the components of leaves from M. tomentosa Benth. (Fritsch) (Chrysobalanaceae) and its effects on bacterial cell growth, biofilm production and macrophage activity. Methods The effect of the different leaf extracts against bacterial cell growth was performed using the microdilution method. The most active extract was analyzed by mass spectrometry, and its effect on bacterial biofilm production was evaluated on polystyrene plates. The extract effect on macrophage activity was tested in the RAW264.7 cell line, which was stimulated with different concentrations of the extract in the presence or absence of LPS. Results We show that the ethyl acetate (EtOAc) extract was the most effective against bacterial cell growth. EtOAc extract DI-ESI (-)MSn analysis showed the presence of a glycosylated flavonoid tentatively assigned as myricetin 3-O-xylosyl-rhamnoside (MW 596). Also, the EtOAc extract increased biofilm formation by S. aureus and inhibited cytokine and NO production induced by LPS in RAW macrophages. Conclusion M. tomentosa flavonoid-enriched EtOAc extract presented a bactericidal and anti-inflammatory pharmacological potential.https://doi.org/10.1186/s12906-023-03968-zFlavonoid-O-glycosidesMass spectrometryAntibacterial activityNatural productsBiofilmCytokines |
| spellingShingle | Mariana Freire Campos Leopoldo Clemente Baratto Vinícius Mendes Vidal Ivana Ventura Nascimento Brendo Araujo Gomes Genes de Lima Martins Neto Priscilla Christina Olsen Rodrigo Ribeiro Tarjano Leo Lilian Oliveira Moreira Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract BMC Complementary Medicine and Therapies Flavonoid-O-glycosides Mass spectrometry Antibacterial activity Natural products Biofilm Cytokines |
| title | Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract |
| title_full | Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract |
| title_fullStr | Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract |
| title_full_unstemmed | Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract |
| title_short | Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth. flavonoid-rich leaf extract |
| title_sort | bactericidal and anti inflammatory effects of moquilea tomentosa benth flavonoid rich leaf extract |
| topic | Flavonoid-O-glycosides Mass spectrometry Antibacterial activity Natural products Biofilm Cytokines |
| url | https://doi.org/10.1186/s12906-023-03968-z |
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