Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
The porcine cytomegalovirus (PCMV) is a herpesvirus that may pose a risk for xenotransplantation using pig cells, tissues, or organs. Here, three orthotopic pig heart transplantations into baboons were studied. To detect PCMV, a real-time PCR and a Western blot assay based on four PCMV protein seque...
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2018-02-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/2/66 |
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| author | Uwe Fiebig Jan-Michael Abicht Tanja Mayr Matthias Längin Andrea Bähr Sonja Guethoff Almuth Falkenau Eckhard Wolf Bruno Reichart Tomoyuki Shibahara Joachim Denner |
| author_facet | Uwe Fiebig Jan-Michael Abicht Tanja Mayr Matthias Längin Andrea Bähr Sonja Guethoff Almuth Falkenau Eckhard Wolf Bruno Reichart Tomoyuki Shibahara Joachim Denner |
| author_sort | Uwe Fiebig |
| collection | DOAJ |
| description | The porcine cytomegalovirus (PCMV) is a herpesvirus that may pose a risk for xenotransplantation using pig cells, tissues, or organs. Here, three orthotopic pig heart transplantations into baboons were studied. To detect PCMV, a real-time PCR and a Western blot assay based on four PCMV protein sequences, including two tegument proteins, were used. The transmission of PCMV from the donor pig to the recipient baboon was found in two cases, despite PCMV not being detected in the blood of the donor pigs by real-time PCR. Although it was not in the blood, PCMV was detected in different organs of the donor pigs, and in sibling animals. Immunohistochemistry using an antiserum that is specific for PCMV detected virus protein-expressing cells in all of the organs of the recipient baboon, most likely representing disseminated pig cells. Therefore, for the first time, the distribution of PCMV in organs of the donor pigs and the recipient baboons was described. In addition, baboon cytomegalovirus (BaCMV) was found activated in the recipient, and a screening for hepatitis E virus (HEV) and porcine lymphotropic herpesviruses (PLHV) was performed. For the first time, a cross-reactivity between antibodies directed against PCMV and BaCMV was found. |
| first_indexed | 2024-12-13T19:13:05Z |
| format | Article |
| id | doaj.art-ecd547372cf44f27908b2578ad253e87 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T19:13:05Z |
| publishDate | 2018-02-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ecd547372cf44f27908b2578ad253e872022-12-21T23:34:22ZengMDPI AGViruses1999-49152018-02-011026610.3390/v10020066v10020066Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart TransplantationUwe Fiebig0Jan-Michael Abicht1Tanja Mayr2Matthias Längin3Andrea Bähr4Sonja Guethoff5Almuth Falkenau6Eckhard Wolf7Bruno Reichart8Tomoyuki Shibahara9Joachim Denner10Department of HIV and other Retroviruses, Robert Koch Institute, 13535 Berlin, GermanyDepartment of Anaesthesiology, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyDepartment of Anaesthesiology, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyDepartment of Anaesthesiology, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyMolecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, 85764 Oberschleißheim, GermanyWalter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyInstitute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyMolecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universität München, 85764 Oberschleißheim, GermanyWalter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, 81377 Munich, GermanyPathology and Pathophysiology Research Division, National Institute of Animal Health, Tsukuba 305-0856, JapanRobert Koch Fellow, Robert Koch Institute, 13353 Berlin, GermanyThe porcine cytomegalovirus (PCMV) is a herpesvirus that may pose a risk for xenotransplantation using pig cells, tissues, or organs. Here, three orthotopic pig heart transplantations into baboons were studied. To detect PCMV, a real-time PCR and a Western blot assay based on four PCMV protein sequences, including two tegument proteins, were used. The transmission of PCMV from the donor pig to the recipient baboon was found in two cases, despite PCMV not being detected in the blood of the donor pigs by real-time PCR. Although it was not in the blood, PCMV was detected in different organs of the donor pigs, and in sibling animals. Immunohistochemistry using an antiserum that is specific for PCMV detected virus protein-expressing cells in all of the organs of the recipient baboon, most likely representing disseminated pig cells. Therefore, for the first time, the distribution of PCMV in organs of the donor pigs and the recipient baboons was described. In addition, baboon cytomegalovirus (BaCMV) was found activated in the recipient, and a screening for hepatitis E virus (HEV) and porcine lymphotropic herpesviruses (PLHV) was performed. For the first time, a cross-reactivity between antibodies directed against PCMV and BaCMV was found.http://www.mdpi.com/1999-4915/10/2/66porcine cytomegalovirusvirus transmissionxenotransplantationvirus safety |
| spellingShingle | Uwe Fiebig Jan-Michael Abicht Tanja Mayr Matthias Längin Andrea Bähr Sonja Guethoff Almuth Falkenau Eckhard Wolf Bruno Reichart Tomoyuki Shibahara Joachim Denner Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation Viruses porcine cytomegalovirus virus transmission xenotransplantation virus safety |
| title | Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation |
| title_full | Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation |
| title_fullStr | Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation |
| title_full_unstemmed | Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation |
| title_short | Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation |
| title_sort | distribution of porcine cytomegalovirus in infected donor pigs and in baboon recipients of pig heart transplantation |
| topic | porcine cytomegalovirus virus transmission xenotransplantation virus safety |
| url | http://www.mdpi.com/1999-4915/10/2/66 |
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