Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran
Background: Sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. In the current study, we investigated the modulation of Phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors. Methods: Sand flies were grouped according to...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-02-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/290 |
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| author | Nasibeh Hosseini-Vasoukolaei AhmadReza Mahmoudi Ali Khamesipour MohammadReza Yaghoobi-Ershadi Shaden Kamhawi Jesus G Valenzuela MohammadHossein Arandian Hossein Mirhendi Shaghayegh Emami Zahra Saeidi Farah Idali Reza Jafari Mahmood Jeddi-Tehrani AmirAhmad Akhavan |
| author_facet | Nasibeh Hosseini-Vasoukolaei AhmadReza Mahmoudi Ali Khamesipour MohammadReza Yaghoobi-Ershadi Shaden Kamhawi Jesus G Valenzuela MohammadHossein Arandian Hossein Mirhendi Shaghayegh Emami Zahra Saeidi Farah Idali Reza Jafari Mahmood Jeddi-Tehrani AmirAhmad Akhavan |
| author_sort | Nasibeh Hosseini-Vasoukolaei |
| collection | DOAJ |
| description | Background: Sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. In the current study, we investigated the modulation of Phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors.
Methods: Sand flies were grouped according to physiological stages such as unfed, fed, semi-gravid, gravid, parous, nulliparous, infected or non-infected with Leishmania major and based on the season in which they were collected. Salivary gland antigens (SGAs) were analyzed using SDS-PAGE and the antibody response against SGAs in Rhombomys opimus was determined by ELISA and Western blot.
Results: The highest protein content was found in the salivary glands of unfed sand flies. The saliva content was higher in parous compared to nulliparous, in summer compared to spring, and in Leishmania-infected compared to non-infected flies. The salivary gland lysate (SGL) electrophoretic pattern variations were observed among sand flies with various physiological stages particularly from 4–9 protein bands of 14–70 kDa. The SGL of unfed and gravid flies had extra protein bands compared to fed and semi-gravid sand flies. There was missing protein bands in SGL of parous compared to nulliparous; and in summer compared to spring collected flies. Rhombomys opimus serum reacted strongly with an antigenic band of around 28 kDa in the SGL of all sand fly groups.
Conclusion: Certain biological and environmental characteristics of wild populations of vector sand flies affect the protein content and antigenicity of saliva. This might have an important implication in the design of vector-based vaccines. |
| first_indexed | 2024-12-16T23:07:38Z |
| format | Article |
| id | doaj.art-ecd55e6f349a4eaabad2934905a700f9 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-16T23:07:38Z |
| publishDate | 2016-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-ecd55e6f349a4eaabad2934905a700f92022-12-21T22:12:31ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712016-02-01101268Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central IranNasibeh Hosseini-Vasoukolaei0AhmadReza Mahmoudi1Ali Khamesipour2MohammadReza Yaghoobi-Ershadi3Shaden Kamhawi4Jesus G Valenzuela5MohammadHossein Arandian6Hossein Mirhendi7Shaghayegh Emami8Zahra Saeidi9Farah Idali10Reza Jafari11Mahmood Jeddi-Tehrani12AmirAhmad Akhavan13Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, USA.Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, USA.Esfahan Health Research Station, National Institute of Health Research, Tehran University of Medical SciencesEsfahan, Iran.Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Esfahan Health Research Station, National Institute of Health Research, Tehran University of Medical SciencesEsfahan, Iran.Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.Background: Sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. In the current study, we investigated the modulation of Phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors. Methods: Sand flies were grouped according to physiological stages such as unfed, fed, semi-gravid, gravid, parous, nulliparous, infected or non-infected with Leishmania major and based on the season in which they were collected. Salivary gland antigens (SGAs) were analyzed using SDS-PAGE and the antibody response against SGAs in Rhombomys opimus was determined by ELISA and Western blot. Results: The highest protein content was found in the salivary glands of unfed sand flies. The saliva content was higher in parous compared to nulliparous, in summer compared to spring, and in Leishmania-infected compared to non-infected flies. The salivary gland lysate (SGL) electrophoretic pattern variations were observed among sand flies with various physiological stages particularly from 4–9 protein bands of 14–70 kDa. The SGL of unfed and gravid flies had extra protein bands compared to fed and semi-gravid sand flies. There was missing protein bands in SGL of parous compared to nulliparous; and in summer compared to spring collected flies. Rhombomys opimus serum reacted strongly with an antigenic band of around 28 kDa in the SGL of all sand fly groups. Conclusion: Certain biological and environmental characteristics of wild populations of vector sand flies affect the protein content and antigenicity of saliva. This might have an important implication in the design of vector-based vaccines.https://jad.tums.ac.ir/index.php/jad/article/view/290Antibody responsePhlebotomus papatasiRhombomys opimusSalivary gland antigensIran |
| spellingShingle | Nasibeh Hosseini-Vasoukolaei AhmadReza Mahmoudi Ali Khamesipour MohammadReza Yaghoobi-Ershadi Shaden Kamhawi Jesus G Valenzuela MohammadHossein Arandian Hossein Mirhendi Shaghayegh Emami Zahra Saeidi Farah Idali Reza Jafari Mahmood Jeddi-Tehrani AmirAhmad Akhavan Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran Journal of Arthropod-Borne Diseases Antibody response Phlebotomus papatasi Rhombomys opimus Salivary gland antigens Iran |
| title | Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran |
| title_full | Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran |
| title_fullStr | Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran |
| title_full_unstemmed | Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran |
| title_short | Seasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran |
| title_sort | seasonal and physiological variations of phlebotomus papatasi salivary gland antigens in central iran |
| topic | Antibody response Phlebotomus papatasi Rhombomys opimus Salivary gland antigens Iran |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/290 |
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