Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles
Summary: Plasmodium falciparum, a human malaria parasite, develops in red blood cells (RBCs), which represent approximately 70% of all human blood cells. Additionally, RBC-derived extracellular vesicles (RBC-EVs) represent 7.3% of the total EV population. The roles of microRNAs (miRNAs) in the conse...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011963 |
| _version_ | 1797774582888792064 |
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| author | Yifan Wu Stephanie Leyk Hanifeh Torabi Katharina Höhn Barbara Honecker Maria del Pilar Martinez Tauler Dániel Cadar Thomas Jacobs Iris Bruchhaus Nahla Galal Metwally |
| author_facet | Yifan Wu Stephanie Leyk Hanifeh Torabi Katharina Höhn Barbara Honecker Maria del Pilar Martinez Tauler Dániel Cadar Thomas Jacobs Iris Bruchhaus Nahla Galal Metwally |
| author_sort | Yifan Wu |
| collection | DOAJ |
| description | Summary: Plasmodium falciparum, a human malaria parasite, develops in red blood cells (RBCs), which represent approximately 70% of all human blood cells. Additionally, RBC-derived extracellular vesicles (RBC-EVs) represent 7.3% of the total EV population. The roles of microRNAs (miRNAs) in the consequences of P. falciparum infection are unclear. Here, we analyzed the miRNA profiles of non-infected human RBCs (niRBCs), ring-infected RBCs (riRBCs), and trophozoite-infected RBCs (trRBCs), as well as those of EVs secreted from these cells. Hsa-miR-451a was the most abundant miRNA in all RBC and RBC-EV populations, but its expression level was not affected by P. falciparum infection. Overall, the miRNA profiles of RBCs and their EVs were altered significantly after infection. Most of the differentially expressed miRNAs were shared between RBCs and their EVs. A target prediction analysis of the miRNAs revealed the possible identity of the genes targeted by these miRNAs (CXCL10, OAS1, IL7, and CCL5) involved in immunomodulation. |
| first_indexed | 2024-03-12T22:23:12Z |
| format | Article |
| id | doaj.art-ecd89956273b4f2c80e0baf99a922a96 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:23:12Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ecd89956273b4f2c80e0baf99a922a962023-07-23T04:55:36ZengElsevieriScience2589-00422023-07-01267107119Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesiclesYifan Wu0Stephanie Leyk1Hanifeh Torabi2Katharina Höhn3Barbara Honecker4Maria del Pilar Martinez Tauler5Dániel Cadar6Thomas Jacobs7Iris Bruchhaus8Nahla Galal Metwally9Research Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Protozoa Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyCellular Parasitology Department, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyArbovirology Department, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Protozoa Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, GermanyResearch Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; Biology Department University of Hamburg, Hamburg, GermanyResearch Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; Corresponding authorSummary: Plasmodium falciparum, a human malaria parasite, develops in red blood cells (RBCs), which represent approximately 70% of all human blood cells. Additionally, RBC-derived extracellular vesicles (RBC-EVs) represent 7.3% of the total EV population. The roles of microRNAs (miRNAs) in the consequences of P. falciparum infection are unclear. Here, we analyzed the miRNA profiles of non-infected human RBCs (niRBCs), ring-infected RBCs (riRBCs), and trophozoite-infected RBCs (trRBCs), as well as those of EVs secreted from these cells. Hsa-miR-451a was the most abundant miRNA in all RBC and RBC-EV populations, but its expression level was not affected by P. falciparum infection. Overall, the miRNA profiles of RBCs and their EVs were altered significantly after infection. Most of the differentially expressed miRNAs were shared between RBCs and their EVs. A target prediction analysis of the miRNAs revealed the possible identity of the genes targeted by these miRNAs (CXCL10, OAS1, IL7, and CCL5) involved in immunomodulation.http://www.sciencedirect.com/science/article/pii/S2589004223011963ParasitologyCell biology |
| spellingShingle | Yifan Wu Stephanie Leyk Hanifeh Torabi Katharina Höhn Barbara Honecker Maria del Pilar Martinez Tauler Dániel Cadar Thomas Jacobs Iris Bruchhaus Nahla Galal Metwally Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles iScience Parasitology Cell biology |
| title | Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles |
| title_full | Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles |
| title_fullStr | Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles |
| title_full_unstemmed | Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles |
| title_short | Plasmodium falciparum infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles |
| title_sort | plasmodium falciparum infection reshapes the human microrna profiles of red blood cells and their extracellular vesicles |
| topic | Parasitology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223011963 |
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