A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

A TGFβ/BMP receptor library with Halo- and SNAP-tags is generated and with the application of labeled growth factor ligands, a ligand surface binding assay is developed, allowing assessment of preferential ligand-receptor binding in cells.

Bibliographic Details
Main Authors: Jerome Jatzlau, Wiktor Burdzinski, Michael Trumpp, Leon Obendorf, Kilian Roßmann, Katharina Ravn, Marko Hyvönen, Francesca Bottanelli, Johannes Broichhagen, Petra Knaus
Format: Article
Language:English
Published: Nature Portfolio 2023-01-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-04388-4