Introduction of Large Sequence Inserts by CRISPR-Cas9 To Create Pathogenicity Mutants in the Multinucleate Filamentous Pathogen <named-content content-type="genus-species">Sclerotinia sclerotiorum</named-content>

Introduction of Large Sequence Inserts by CRISPR-Cas9 To Create Pathogenicity Mutants in the Multinucleate Filamentous Pathogen <named-content content-type="genus-species">Sclerotinia sclerotiorum</named-content>

ABSTRACT The necrotrophic fungal plant pathogen Sclerotinia sclerotiorum is responsible for substantial global crop losses annually resulting in localized food insecurity and loss of livelihood. Understanding the basis of this broad-host-range and aggressive pathogenicity is hampered by the quantita...

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Bibliographic Details
Main Authors: Jingtao Li, Yanhua Zhang, Yucheng Zhang, Pei-Ling Yu, Hongyu Pan, Jeffrey A. Rollins
Format: Article
Language:English
Published: American Society for Microbiology 2018-07-01
Series:mBio
Subjects:
CRISPR
Cas9
filamentous fungi
functional genomics
gene disruption
necrotroph
Online Access:https://journals.asm.org/doi/10.1128/mBio.00567-18

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