Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss
Early pregnancy loss (EPL) is estimated to be between 15 and 20% of all adverse pregnancies. Approximately, half of EPL cases have no identifiable cause. Herein, we apply atomic force microscopy to evaluate the alteration of morphology and nanomechanics of erythrocytes from women with EPL with unkno...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/9/4512 |
| _version_ | 1797504743727169536 |
|---|---|
| author | Ariana Langari Velichka Strijkova Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Stefka G. Taneva Ina Giosheva Emil Gartchev Kamelia Kercheva Alexey Savov Svetla Todinova |
| author_facet | Ariana Langari Velichka Strijkova Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Stefka G. Taneva Ina Giosheva Emil Gartchev Kamelia Kercheva Alexey Savov Svetla Todinova |
| author_sort | Ariana Langari |
| collection | DOAJ |
| description | Early pregnancy loss (EPL) is estimated to be between 15 and 20% of all adverse pregnancies. Approximately, half of EPL cases have no identifiable cause. Herein, we apply atomic force microscopy to evaluate the alteration of morphology and nanomechanics of erythrocytes from women with EPL with unknown etiology, as compared to healthy pregnant (PC) and nonpregnant women (NPC). Freshly isolated erythrocytes from women with EPL differ in both the roughness value (4.6 ± 0.3 nm, <i>p</i> < 0.05), and Young’s modulus (2.54 ± 0.6 MPa, <i>p</i> < 0.01) compared to the values for NPC (3.8 ± 0.4 nm and 0.94 ± 0.2 MPa, respectively) and PC (3.3 ± 0.2 nm and 1.12 ± 0.3 MPa, respectively). Moreover, we find a time-dependent trend for the reduction of the cells’ morphometric parameters (cells size and surface roughness) and the membrane elasticity—much faster for EPL than for the two control groups. The accelerated aging of EPL erythrocytes is expressed in faster morphological shape transformation and earlier occurrence of spiculated and spherical-shaped cells, reduced membrane roughness and elasticity with aging evolution. Oxidative stress in vitro contributed to the morphological cells’ changes observed for EPL senescent erythrocytes. The ultrastructural characteristics of cells derived from women with miscarriages show potential as a supplementary mark for a pathological state. |
| first_indexed | 2024-03-10T04:08:41Z |
| format | Article |
| id | doaj.art-ecd9d93237ca421bb28a1f0ae8450e83 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:08:41Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ecd9d93237ca421bb28a1f0ae8450e832023-11-23T08:18:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239451210.3390/ijms23094512Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy LossAriana Langari0Velichka Strijkova1Regina Komsa-Penkova2Avgustina Danailova3Sashka Krumova4Stefka G. Taneva5Ina Giosheva6Emil Gartchev7Kamelia Kercheva8Alexey Savov9Svetla Todinova10Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaDepartment of Biochemistry, Medical University—Pleven, Sv. Kliment Ohridski Str. 1, 5800 Pleven, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaEarly pregnancy loss (EPL) is estimated to be between 15 and 20% of all adverse pregnancies. Approximately, half of EPL cases have no identifiable cause. Herein, we apply atomic force microscopy to evaluate the alteration of morphology and nanomechanics of erythrocytes from women with EPL with unknown etiology, as compared to healthy pregnant (PC) and nonpregnant women (NPC). Freshly isolated erythrocytes from women with EPL differ in both the roughness value (4.6 ± 0.3 nm, <i>p</i> < 0.05), and Young’s modulus (2.54 ± 0.6 MPa, <i>p</i> < 0.01) compared to the values for NPC (3.8 ± 0.4 nm and 0.94 ± 0.2 MPa, respectively) and PC (3.3 ± 0.2 nm and 1.12 ± 0.3 MPa, respectively). Moreover, we find a time-dependent trend for the reduction of the cells’ morphometric parameters (cells size and surface roughness) and the membrane elasticity—much faster for EPL than for the two control groups. The accelerated aging of EPL erythrocytes is expressed in faster morphological shape transformation and earlier occurrence of spiculated and spherical-shaped cells, reduced membrane roughness and elasticity with aging evolution. Oxidative stress in vitro contributed to the morphological cells’ changes observed for EPL senescent erythrocytes. The ultrastructural characteristics of cells derived from women with miscarriages show potential as a supplementary mark for a pathological state.https://www.mdpi.com/1422-0067/23/9/4512erythrocytesearly pregnancy lossatomic force microscopycells’ senescencemembrane roughnessYoung’s modulus |
| spellingShingle | Ariana Langari Velichka Strijkova Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Stefka G. Taneva Ina Giosheva Emil Gartchev Kamelia Kercheva Alexey Savov Svetla Todinova Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss International Journal of Molecular Sciences erythrocytes early pregnancy loss atomic force microscopy cells’ senescence membrane roughness Young’s modulus |
| title | Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss |
| title_full | Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss |
| title_fullStr | Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss |
| title_full_unstemmed | Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss |
| title_short | Morphometric and Nanomechanical Features of Erythrocytes Characteristic of Early Pregnancy Loss |
| title_sort | morphometric and nanomechanical features of erythrocytes characteristic of early pregnancy loss |
| topic | erythrocytes early pregnancy loss atomic force microscopy cells’ senescence membrane roughness Young’s modulus |
| url | https://www.mdpi.com/1422-0067/23/9/4512 |
| work_keys_str_mv | AT arianalangari morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT velichkastrijkova morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT reginakomsapenkova morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT avgustinadanailova morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT sashkakrumova morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT stefkagtaneva morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT inagiosheva morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT emilgartchev morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT kameliakercheva morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT alexeysavov morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss AT svetlatodinova morphometricandnanomechanicalfeaturesoferythrocytescharacteristicofearlypregnancyloss |