Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers
In diabetic foot ulcers (DFUs), biofilm formation is a major challenge that promotes wound chronicity and delays healing. Antiseptics have been proposed to combat biofilms in the management of DFUs. However, there is limited evidence on the activity of these agents against biofilms, and there are qu...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11270 |
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| author | Koko Barrigah-Benissan Jerome Ory Catherine Dunyach-Remy Cassandra Pouget Jean-Philippe Lavigne Albert Sotto |
| author_facet | Koko Barrigah-Benissan Jerome Ory Catherine Dunyach-Remy Cassandra Pouget Jean-Philippe Lavigne Albert Sotto |
| author_sort | Koko Barrigah-Benissan |
| collection | DOAJ |
| description | In diabetic foot ulcers (DFUs), biofilm formation is a major challenge that promotes wound chronicity and delays healing. Antiseptics have been proposed to combat biofilms in the management of DFUs. However, there is limited evidence on the activity of these agents against biofilms, and there are questions as to which agents have the best efficiency. Here, we evaluated the antibiofilm activity of sodium hypochlorite, polyvinylpyrrolidoneIodine (PVPI), polyhexamethylenebiguanide (PHMB) and octenidine against <i>Pseudomonas aeruginosa</i> strains using static and dynamic systems in a chronic-wound-like medium (CWM) that mimics the chronic wound environment. Using Antibiofilmogram<sup>®</sup>, a technology assessing the ability of antiseptics to reduce the initial phase of biofilm formation, we observed the significant activity of antiseptics against biofilm formation by <i>P. aeruginosa</i> (at 1:40 to 1:8 dilutions). Moreover, 1:100 to 1:3 dilutions of the different antiseptics reduced mature biofilms formed after 72 h by 10-log, although higher concentrations were needed in CWM (1:40 to 1:2). Finally, in the BioFlux200<sup>TM</sup> model, after biofilm debridement, sodium hypochlorite and PHMB were the most effective antiseptics. In conclusion, our study showed that among the four antiseptics tested, sodium hypochlorite demonstrated the best antibiofilm activity against <i>P. aeruginosa</i> biofilms and represents an alternative in the management of DFUs. |
| first_indexed | 2024-03-09T21:41:25Z |
| format | Article |
| id | doaj.art-ecda54f5459c456c8869c06968478958 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:41:25Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ecda54f5459c456c8869c069684789582023-11-23T20:30:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191127010.3390/ijms231911270Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot UlcersKoko Barrigah-Benissan0Jerome Ory1Catherine Dunyach-Remy2Cassandra Pouget3Jean-Philippe Lavigne4Albert Sotto5Bacterial Virulence and Chronic Infections, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Department of Microbiology and Hospital Hygiene, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceBacterial Virulence and Chronic Infections, INSERM U1047, Department of Infectious Diseases, CHU Nîmes, Univ Montpellier, Place du Pr Debré, 30029 Nîmes, FranceIn diabetic foot ulcers (DFUs), biofilm formation is a major challenge that promotes wound chronicity and delays healing. Antiseptics have been proposed to combat biofilms in the management of DFUs. However, there is limited evidence on the activity of these agents against biofilms, and there are questions as to which agents have the best efficiency. Here, we evaluated the antibiofilm activity of sodium hypochlorite, polyvinylpyrrolidoneIodine (PVPI), polyhexamethylenebiguanide (PHMB) and octenidine against <i>Pseudomonas aeruginosa</i> strains using static and dynamic systems in a chronic-wound-like medium (CWM) that mimics the chronic wound environment. Using Antibiofilmogram<sup>®</sup>, a technology assessing the ability of antiseptics to reduce the initial phase of biofilm formation, we observed the significant activity of antiseptics against biofilm formation by <i>P. aeruginosa</i> (at 1:40 to 1:8 dilutions). Moreover, 1:100 to 1:3 dilutions of the different antiseptics reduced mature biofilms formed after 72 h by 10-log, although higher concentrations were needed in CWM (1:40 to 1:2). Finally, in the BioFlux200<sup>TM</sup> model, after biofilm debridement, sodium hypochlorite and PHMB were the most effective antiseptics. In conclusion, our study showed that among the four antiseptics tested, sodium hypochlorite demonstrated the best antibiofilm activity against <i>P. aeruginosa</i> biofilms and represents an alternative in the management of DFUs.https://www.mdpi.com/1422-0067/23/19/11270antibiofilmAntibiofilmogram<sup>®</sup>antisepticbiofilmchronic wound<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Koko Barrigah-Benissan Jerome Ory Catherine Dunyach-Remy Cassandra Pouget Jean-Philippe Lavigne Albert Sotto Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers International Journal of Molecular Sciences antibiofilm Antibiofilmogram<sup>®</sup> antiseptic biofilm chronic wound <i>Pseudomonas aeruginosa</i> |
| title | Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers |
| title_full | Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers |
| title_fullStr | Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers |
| title_full_unstemmed | Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers |
| title_short | Antibiofilm Properties of Antiseptic Agents Used on <i>Pseudomonas aeruginosa</i> Isolated from Diabetic Foot Ulcers |
| title_sort | antibiofilm properties of antiseptic agents used on i pseudomonas aeruginosa i isolated from diabetic foot ulcers |
| topic | antibiofilm Antibiofilmogram<sup>®</sup> antiseptic biofilm chronic wound <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/1422-0067/23/19/11270 |
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