Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524
An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The...
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| Format: | Article |
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MDPI AG
2011-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/9/1/109/ |
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| author | Bin-Bin Xie Yu-Zhong Zhang Xiu-Lan Chen Chun-Bo Li Jie Song Sheng Dong Jian-Wei Li |
| author_facet | Bin-Bin Xie Yu-Zhong Zhang Xiu-Lan Chen Chun-Bo Li Jie Song Sheng Dong Jian-Wei Li |
| author_sort | Bin-Bin Xie |
| collection | DOAJ |
| description | An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The bifunctional alginate lyasealy-SJ02 secreted by strain SM0524 was purified. Aly-SJ02 had an apparent molecular mass of 32 kDa. The optimal temperature and pH of aly-SJ02 toward sodium alginate was 50 °C and 8.5, respectively. The half life period of aly-SJ02 was 41 min at 40 °C and 20 min at 50 °C. Aly-SJ02 was most stable at pH 8.0. N-terminal sequence analysis suggested that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18. Aly-SJ02 showed activities toward both polyG (α-L-guluronic acid) and polyM (β-D-mannuronic acid), indicating that it is a bifunctional alginate lyase. Aly-SJ02 had lower Km toward polyG than toward polyM and sodium alginate. Thin layer chromatography and ESI-MS analyses showed that aly-SJ02 mainly released dimers and trimers from polyM and alginate, and trimers and tetramers from polyG, which suggests that aly-SJ02 may be a good tool to produce dimers and trimers from alginate. |
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| issn | 1660-3397 |
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| last_indexed | 2024-04-11T13:27:19Z |
| publishDate | 2011-01-01 |
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| series | Marine Drugs |
| spelling | doaj.art-ecdea8db4bf24f00b96970e1c19fa5ab2022-12-22T04:22:01ZengMDPI AGMarine Drugs1660-33972011-01-019110912310.3390/md9010109Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524Bin-Bin XieYu-Zhong ZhangXiu-Lan ChenChun-Bo LiJie SongSheng DongJian-Wei LiAn alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The bifunctional alginate lyasealy-SJ02 secreted by strain SM0524 was purified. Aly-SJ02 had an apparent molecular mass of 32 kDa. The optimal temperature and pH of aly-SJ02 toward sodium alginate was 50 °C and 8.5, respectively. The half life period of aly-SJ02 was 41 min at 40 °C and 20 min at 50 °C. Aly-SJ02 was most stable at pH 8.0. N-terminal sequence analysis suggested that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18. Aly-SJ02 showed activities toward both polyG (α-L-guluronic acid) and polyM (β-D-mannuronic acid), indicating that it is a bifunctional alginate lyase. Aly-SJ02 had lower Km toward polyG than toward polyM and sodium alginate. Thin layer chromatography and ESI-MS analyses showed that aly-SJ02 mainly released dimers and trimers from polyM and alginate, and trimers and tetramers from polyG, which suggests that aly-SJ02 may be a good tool to produce dimers and trimers from alginate.http://www.mdpi.com/1660-3397/9/1/109/bifunctional alginate lyasePseudoalteromonas sp. SM0524alginate |
| spellingShingle | Bin-Bin Xie Yu-Zhong Zhang Xiu-Lan Chen Chun-Bo Li Jie Song Sheng Dong Jian-Wei Li Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 Marine Drugs bifunctional alginate lyase Pseudoalteromonas sp. SM0524 alginate |
| title | Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 |
| title_full | Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 |
| title_fullStr | Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 |
| title_full_unstemmed | Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 |
| title_short | Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524 |
| title_sort | purification and characterization of a bifunctional alginate lyase from pseudoalteromonas sp sm0524 |
| topic | bifunctional alginate lyase Pseudoalteromonas sp. SM0524 alginate |
| url | http://www.mdpi.com/1660-3397/9/1/109/ |
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