New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes
The incidence of type 1 diabetes (T1D) has been dramatically increased in developed countries, and beyond the genetic impact, environmental factors, including diet, seem to play an important role in the onset and development of the disease. In this vein, five <i>Lacticaseibacillus</i> &l...
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| स्वरूप: | लेख |
| भाषा: | English |
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MDPI AG
2022-01-01
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| श्रृंखला: | Microorganisms |
| विषय: | |
| ऑनलाइन पहुंच: | https://www.mdpi.com/2076-2607/10/2/272 |
| _version_ | 1827653760406519808 |
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| author | Grigorios Nelios Valentini Santarmaki Chrysoula Pavlatou Dimitra Dimitrellou Yiannis Kourkoutas |
| author_facet | Grigorios Nelios Valentini Santarmaki Chrysoula Pavlatou Dimitra Dimitrellou Yiannis Kourkoutas |
| author_sort | Grigorios Nelios |
| collection | DOAJ |
| description | The incidence of type 1 diabetes (T1D) has been dramatically increased in developed countries, and beyond the genetic impact, environmental factors, including diet, seem to play an important role in the onset and development of the disease. In this vein, five <i>Lacticaseibacillus</i> <i>rhamnosus</i>, isolated from traditional fermented Greek products, were screened for potential probiotic properties, aiming at maintaining gut homeostasis and antidiabetic capability to alleviate T1D symptoms. <i>L. rhamnosus</i> cell-free supernatants induced strong growth inhibitory activity against common food spoilage and foodborne pathogenic microorganisms, associated with several diseases, including T1D, and were also able to inhibit <i>α</i>-glucosidase activity (up to 44.87%), a promising property for alternatives to the antidiabetic drugs. In addition, survival rates up to 36.76% were recorded during the application of the static in vitro digestion model. The strains had no hemolytic activity and were sensitive to common antibiotics suggested by the European Food and Safety Association, apart from chloramphenicol. However, it is highly unlikely that the resistance has been acquired. In conclusion, our results suggest a great health-promoting potential of the newly isolated wild-type <i>L. rhamnosus</i> strains, but further confirmation of their efficiency in experimental animal models is considered an essential next research step. |
| first_indexed | 2024-03-09T21:24:13Z |
| format | Article |
| id | doaj.art-ece2bc3f8a0a42c4b9e24fe40f8aa2c0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:24:13Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ece2bc3f8a0a42c4b9e24fe40f8aa2c02023-11-23T21:13:30ZengMDPI AGMicroorganisms2076-26072022-01-0110227210.3390/microorganisms10020272New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 DiabetesGrigorios Nelios0Valentini Santarmaki1Chrysoula Pavlatou2Dimitra Dimitrellou3Yiannis Kourkoutas4Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Food Science and Technology, Ionian University, 28100 Argostoli, GreeceLaboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, GreeceThe incidence of type 1 diabetes (T1D) has been dramatically increased in developed countries, and beyond the genetic impact, environmental factors, including diet, seem to play an important role in the onset and development of the disease. In this vein, five <i>Lacticaseibacillus</i> <i>rhamnosus</i>, isolated from traditional fermented Greek products, were screened for potential probiotic properties, aiming at maintaining gut homeostasis and antidiabetic capability to alleviate T1D symptoms. <i>L. rhamnosus</i> cell-free supernatants induced strong growth inhibitory activity against common food spoilage and foodborne pathogenic microorganisms, associated with several diseases, including T1D, and were also able to inhibit <i>α</i>-glucosidase activity (up to 44.87%), a promising property for alternatives to the antidiabetic drugs. In addition, survival rates up to 36.76% were recorded during the application of the static in vitro digestion model. The strains had no hemolytic activity and were sensitive to common antibiotics suggested by the European Food and Safety Association, apart from chloramphenicol. However, it is highly unlikely that the resistance has been acquired. In conclusion, our results suggest a great health-promoting potential of the newly isolated wild-type <i>L. rhamnosus</i> strains, but further confirmation of their efficiency in experimental animal models is considered an essential next research step.https://www.mdpi.com/2076-2607/10/2/272<i>Lacticaseibacillus rhamnosus</i>type 1 diabetesprobioticsfoodborne pathogens<i>α</i>-glucosidase inhibitory activity |
| spellingShingle | Grigorios Nelios Valentini Santarmaki Chrysoula Pavlatou Dimitra Dimitrellou Yiannis Kourkoutas New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes Microorganisms <i>Lacticaseibacillus rhamnosus</i> type 1 diabetes probiotics foodborne pathogens <i>α</i>-glucosidase inhibitory activity |
| title | New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes |
| title_full | New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes |
| title_fullStr | New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes |
| title_full_unstemmed | New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes |
| title_short | New Wild-Type <i>Lacticaseibacillus rhamnosus</i> Strains as Candidates to Manage Type 1 Diabetes |
| title_sort | new wild type i lacticaseibacillus rhamnosus i strains as candidates to manage type 1 diabetes |
| topic | <i>Lacticaseibacillus rhamnosus</i> type 1 diabetes probiotics foodborne pathogens <i>α</i>-glucosidase inhibitory activity |
| url | https://www.mdpi.com/2076-2607/10/2/272 |
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