Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
Ophiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein fro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055055/full |
| _version_ | 1811179350692724736 |
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| author | Chu-Yu Tang Jie Wang Xin Liu Jian-Bo Chen Jing Liang Tao Wang Wayne Roydon Simpson Yu-Ling Li Xiu-Zhang Li |
| author_facet | Chu-Yu Tang Jie Wang Xin Liu Jian-Bo Chen Jing Liang Tao Wang Wayne Roydon Simpson Yu-Ling Li Xiu-Zhang Li |
| author_sort | Chu-Yu Tang |
| collection | DOAJ |
| description | Ophiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from O. sinensis using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics. The maximum mycelial protein content of 2.11% was obtained using a medium consisting of 20% beef broth, 0.10% peptone, 2% glucose, 0.15% yeast extract, 0.20% KH2PO4, and 0.02% MgSO4. Transcriptome analysis identified 790 differentially expressed genes (DEGs), including 592 up-regulated genes and 198 down-regulated genes, optimisation resulted in more up-regulated genes. The main DEGs were enriched in metabolic pathways, ABC transporters, starch and sucrose metabolism, tyrosine metabolism, and glutathione metabolism. In addition, some DEGs associated with mycelial protein enhancement such as tyrosinase (TYR), glutathione S-transferase (GST), glutamine synthetase (glnA), and β-glucosidase may contribute to increased mycelial protein content. Real-time quantitative PCR (RT-qPCR) was used to confirm gene expression and the results support the accuracy of RNA-Seq and DEG analysis. This study provides an optimized fermentation method for enhancing the mycelial protein content of O. sinensis and a reference for the effective development of O. sinensis protein. |
| first_indexed | 2024-04-11T06:33:16Z |
| format | Article |
| id | doaj.art-ece4eb6bd0ef4debadc616ffa1690ecd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T06:33:16Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ece4eb6bd0ef4debadc616ffa1690ecd2022-12-22T04:39:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10550551055055Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodologyChu-Yu Tang0Jie Wang1Xin Liu2Jian-Bo Chen3Jing Liang4Tao Wang5Wayne Roydon Simpson6Yu-Ling Li7Xiu-Zhang Li8State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaAgResearch Grasslands Research Centre, Palmerston North, New ZealandState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, ChinaOphiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from O. sinensis using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics. The maximum mycelial protein content of 2.11% was obtained using a medium consisting of 20% beef broth, 0.10% peptone, 2% glucose, 0.15% yeast extract, 0.20% KH2PO4, and 0.02% MgSO4. Transcriptome analysis identified 790 differentially expressed genes (DEGs), including 592 up-regulated genes and 198 down-regulated genes, optimisation resulted in more up-regulated genes. The main DEGs were enriched in metabolic pathways, ABC transporters, starch and sucrose metabolism, tyrosine metabolism, and glutathione metabolism. In addition, some DEGs associated with mycelial protein enhancement such as tyrosinase (TYR), glutathione S-transferase (GST), glutamine synthetase (glnA), and β-glucosidase may contribute to increased mycelial protein content. Real-time quantitative PCR (RT-qPCR) was used to confirm gene expression and the results support the accuracy of RNA-Seq and DEG analysis. This study provides an optimized fermentation method for enhancing the mycelial protein content of O. sinensis and a reference for the effective development of O. sinensis protein.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055055/fullOphiocordyceps sinensismyceliumresponse surface methodologytranscriptomesoluble protein content |
| spellingShingle | Chu-Yu Tang Jie Wang Xin Liu Jian-Bo Chen Jing Liang Tao Wang Wayne Roydon Simpson Yu-Ling Li Xiu-Zhang Li Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology Frontiers in Microbiology Ophiocordyceps sinensis mycelium response surface methodology transcriptome soluble protein content |
| title | Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology |
| title_full | Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology |
| title_fullStr | Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology |
| title_full_unstemmed | Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology |
| title_short | Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology |
| title_sort | medium optimization for high mycelial soluble protein content of ophiocordyceps sinensis using response surface methodology |
| topic | Ophiocordyceps sinensis mycelium response surface methodology transcriptome soluble protein content |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055055/full |
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