Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments
Tissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/929 |
| _version_ | 1827712557396262912 |
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| author | Carloalberto Petti |
| author_facet | Carloalberto Petti |
| author_sort | Carloalberto Petti |
| collection | DOAJ |
| description | Tissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is susceptible to tissue culture, and often complex media and hormone requirements are needed to achieve successful plant propagations. The availability of new hormones or chemicals acting as hormones are critical to the expansion of tissue culture potentials. Phloroglucinol has been shown to have certain hormone-like properties in a variety of studies. <i>Ornithogalum dubium</i>, an important geophyte species, was used to characterise the potential of phloroglucinol as the sole plant-like hormone in a tissue culture experiment. Tissue culture, plant regeneration, total phenolic and genetic variability were established by applying a variety of methods throughout long-term experiments. Phloroglucinol did induce callus formation and plant regeneration when used as the sole supplement in the media at a rate of 37%, thus demonstrating auxin/cytokines-like properties. Callus formation was of 3 types, friable and cellular, hard and compact, and a mixture of the two. The important finding was that direct somatogenesis did occur albeit more frequently on younger tissue, whereby rates of induction were up to 52%. It is concluded that phloroglucinol acts as a “hormone-like” molecule and can trigger direct embryogenesis without callus formation. |
| first_indexed | 2024-03-10T18:17:26Z |
| format | Article |
| id | doaj.art-ece5d70781eb4300ad902cc8fbe0a988 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:17:26Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ece5d70781eb4300ad902cc8fbe0a9882023-11-20T07:38:38ZengMDPI AGPlants2223-77472020-07-019892910.3390/plants9080929Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term ExperimentsCarloalberto Petti0Institute of Technology Carlow, EnviroCORE, DSH, Kilkenny Road, R93 V960 Carlow, IrelandTissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is susceptible to tissue culture, and often complex media and hormone requirements are needed to achieve successful plant propagations. The availability of new hormones or chemicals acting as hormones are critical to the expansion of tissue culture potentials. Phloroglucinol has been shown to have certain hormone-like properties in a variety of studies. <i>Ornithogalum dubium</i>, an important geophyte species, was used to characterise the potential of phloroglucinol as the sole plant-like hormone in a tissue culture experiment. Tissue culture, plant regeneration, total phenolic and genetic variability were established by applying a variety of methods throughout long-term experiments. Phloroglucinol did induce callus formation and plant regeneration when used as the sole supplement in the media at a rate of 37%, thus demonstrating auxin/cytokines-like properties. Callus formation was of 3 types, friable and cellular, hard and compact, and a mixture of the two. The important finding was that direct somatogenesis did occur albeit more frequently on younger tissue, whereby rates of induction were up to 52%. It is concluded that phloroglucinol acts as a “hormone-like” molecule and can trigger direct embryogenesis without callus formation.https://www.mdpi.com/2223-7747/9/8/929<i>Ornithogalum dubium</i>callus inductionorganogenesisphloroglucinolregenerationphytohormones |
| spellingShingle | Carloalberto Petti Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments Plants <i>Ornithogalum dubium</i> callus induction organogenesis phloroglucinol regeneration phytohormones |
| title | Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments |
| title_full | Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments |
| title_fullStr | Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments |
| title_full_unstemmed | Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments |
| title_short | Phloroglucinol Mediated Plant Regeneration of <i>Ornithogalum dubium</i> as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments |
| title_sort | phloroglucinol mediated plant regeneration of i ornithogalum dubium i as the sole hormone like supplement in plant tissue culture long term experiments |
| topic | <i>Ornithogalum dubium</i> callus induction organogenesis phloroglucinol regeneration phytohormones |
| url | https://www.mdpi.com/2223-7747/9/8/929 |
| work_keys_str_mv | AT carloalbertopetti phloroglucinolmediatedplantregenerationofiornithogalumdubiumiasthesolehormonelikesupplementinplanttissueculturelongtermexperiments |