Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo
Although the fertilized eggs were found to contain microbes in early studies, the detailed composition of yolk microbiota and its influence on embryo intestinal microbiota have not been satisfactorily examined yet. In this study, the yolk microbiota was explored by using 16s rRNA sequencing at diffe...
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MDPI AG
2021-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/1/16 |
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| author | Peng Ding Huichao Liu Yueyue Tong Xi He Xin Yin Yulong Yin Haihan Zhang Zehe Song |
| author_facet | Peng Ding Huichao Liu Yueyue Tong Xi He Xin Yin Yulong Yin Haihan Zhang Zehe Song |
| author_sort | Peng Ding |
| collection | DOAJ |
| description | Although the fertilized eggs were found to contain microbes in early studies, the detailed composition of yolk microbiota and its influence on embryo intestinal microbiota have not been satisfactorily examined yet. In this study, the yolk microbiota was explored by using 16s rRNA sequencing at different developmental stages of the broiler embryo. The results showed that the relative abundance of yolk microbiota was barely changed during embryogenesis. According to the KEGG analysis, the yolk microbiota were functionally related to amino acid, carbohydrate, and lipid metabolisms during chicken embryogenesis. The yolk microbiota influences the embryonic intestinal microbiota through increasing the colonization of Proteobacteria, Firmicutes, and Bacteroidetes in the intestine, particularly. The intestinal microbes of neonatal chicks showed higher proportions of <i>Faecalibacterium</i>, <i>Blautia</i>, <i>Coprococcus</i>, <i>Dorea</i>, and <i>Roseburia</i> compared to the embryonic intestinal microbiota. Our findings might give a better understanding of the composition and developmental change of yolk microbiota and its roles in shaping the intestinal microbiota. |
| first_indexed | 2024-03-10T03:51:45Z |
| format | Article |
| id | doaj.art-ece75d35ccf0407c9da10b7d01a36f15 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T03:51:45Z |
| publishDate | 2021-12-01 |
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| series | Animals |
| spelling | doaj.art-ece75d35ccf0407c9da10b7d01a36f152023-11-23T11:04:29ZengMDPI AGAnimals2076-26152021-12-011211610.3390/ani12010016Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken EmbryoPeng Ding0Huichao Liu1Yueyue Tong2Xi He3Xin Yin4Yulong Yin5Haihan Zhang6Zehe Song7College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaAlthough the fertilized eggs were found to contain microbes in early studies, the detailed composition of yolk microbiota and its influence on embryo intestinal microbiota have not been satisfactorily examined yet. In this study, the yolk microbiota was explored by using 16s rRNA sequencing at different developmental stages of the broiler embryo. The results showed that the relative abundance of yolk microbiota was barely changed during embryogenesis. According to the KEGG analysis, the yolk microbiota were functionally related to amino acid, carbohydrate, and lipid metabolisms during chicken embryogenesis. The yolk microbiota influences the embryonic intestinal microbiota through increasing the colonization of Proteobacteria, Firmicutes, and Bacteroidetes in the intestine, particularly. The intestinal microbes of neonatal chicks showed higher proportions of <i>Faecalibacterium</i>, <i>Blautia</i>, <i>Coprococcus</i>, <i>Dorea</i>, and <i>Roseburia</i> compared to the embryonic intestinal microbiota. Our findings might give a better understanding of the composition and developmental change of yolk microbiota and its roles in shaping the intestinal microbiota.https://www.mdpi.com/2076-2615/12/1/16yolkmicrobiotaintestineembryocolonization |
| spellingShingle | Peng Ding Huichao Liu Yueyue Tong Xi He Xin Yin Yulong Yin Haihan Zhang Zehe Song Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo Animals yolk microbiota intestine embryo colonization |
| title | Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo |
| title_full | Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo |
| title_fullStr | Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo |
| title_full_unstemmed | Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo |
| title_short | Developmental Change of Yolk Microbiota and Its Role on Early Colonization of Intestinal Microbiota in Chicken Embryo |
| title_sort | developmental change of yolk microbiota and its role on early colonization of intestinal microbiota in chicken embryo |
| topic | yolk microbiota intestine embryo colonization |
| url | https://www.mdpi.com/2076-2615/12/1/16 |
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