ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS
Considering the therapeutic potential of medicinal plants as alternatives to antibiotic therapy, the research aims to evaluate the antimicrobial activity of Commiphora leptophloeos against isolates of Staphylococcus spp. from the milk of ruminants with subclinical mastitis. For this, the crude ethan...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2019-08-01
|
| Series: | Ciência Animal Brasileira |
| Online Access: | https://www.revistas.ufg.br/vet/article/view/57228 |
| _version_ | 1819118030797406208 |
|---|---|
| author | Isamara Ferreira da Silva Amanda Leite Guimarães Vinícius Santana Amorim Telma Maria Guedes da Silva Rodolfo de Moraes Peixoto Xirley Pereira Nunes Tânia Maria Sarmento Silva Mateus Matiuzzi da Costa |
| author_facet | Isamara Ferreira da Silva Amanda Leite Guimarães Vinícius Santana Amorim Telma Maria Guedes da Silva Rodolfo de Moraes Peixoto Xirley Pereira Nunes Tânia Maria Sarmento Silva Mateus Matiuzzi da Costa |
| author_sort | Isamara Ferreira da Silva |
| collection | DOAJ |
| description | Considering the therapeutic potential of medicinal plants as alternatives to antibiotic therapy, the research aims to evaluate the antimicrobial activity of Commiphora leptophloeos against isolates of Staphylococcus spp. from the milk of ruminants with subclinical mastitis. For this, the crude ethanolic extract from the bark and leaves of Commiphora leptophloeos was prepared, with these being chemically characterized by HPLC-DAD-MS and by MALDI-TOF. The extracts were then evaluated as to their antimicrobial effects against 60 isolates of Staphylococcus spp. through the broth microdilution technique to determine the minimum bactericidal concentration. In addition, the extracts were evaluated as to their ability to interfere with biofilm formation and with the already established biofilm. Although all tested extracts showed antimicrobial action, lower MBC values were recorded for the bark extract in the concentration 781.2 μg/mL (25/60). The extracts of the bark and leaves were able to interfere with the initial stages of biofilm formation, but there was no interference of the extract on the established biofilm. There was a high sensitivity of Staphylococcus spp. isolates from subclinical mastitis cases in ruminants when subjected to the extracts from bark and leaves of Commiphora leptophloeos, as well as regarding the ability of extracts to interfere in biofilm formation, indicating their potential in the use for ruminant mastitis therapy.
Keywords: Mastitis, Staphylococcus spp., imburana de cambão, phytotherapy, biofilm. |
| first_indexed | 2024-12-22T05:42:24Z |
| format | Article |
| id | doaj.art-eceb4c56c6cb495ca329108fdcd820af |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-12-22T05:42:24Z |
| publishDate | 2019-08-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-eceb4c56c6cb495ca329108fdcd820af2022-12-21T18:37:10ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912019-08-012011457228ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTSIsamara Ferreira da Silva0Amanda Leite Guimarães1Vinícius Santana Amorim2Telma Maria Guedes da Silva3Rodolfo de Moraes PeixotoXirley Pereira Nunes4Tânia Maria Sarmento Silva5Mateus Matiuzzi da Costa6Universidade do Vale do São Francisco, Petrolina, PE, Brazil.Universidade do Vale do São Francisco, Petrolina, PE, Brazil.Universidade do Vale do São Francisco, Petrolina, PE, Brazil.Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Universidade do Vale do São Francisco, Petrolina, PE, Brazil.Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Universidade do Vale do São Francisco, Petrolina, PE, Brazil.Considering the therapeutic potential of medicinal plants as alternatives to antibiotic therapy, the research aims to evaluate the antimicrobial activity of Commiphora leptophloeos against isolates of Staphylococcus spp. from the milk of ruminants with subclinical mastitis. For this, the crude ethanolic extract from the bark and leaves of Commiphora leptophloeos was prepared, with these being chemically characterized by HPLC-DAD-MS and by MALDI-TOF. The extracts were then evaluated as to their antimicrobial effects against 60 isolates of Staphylococcus spp. through the broth microdilution technique to determine the minimum bactericidal concentration. In addition, the extracts were evaluated as to their ability to interfere with biofilm formation and with the already established biofilm. Although all tested extracts showed antimicrobial action, lower MBC values were recorded for the bark extract in the concentration 781.2 μg/mL (25/60). The extracts of the bark and leaves were able to interfere with the initial stages of biofilm formation, but there was no interference of the extract on the established biofilm. There was a high sensitivity of Staphylococcus spp. isolates from subclinical mastitis cases in ruminants when subjected to the extracts from bark and leaves of Commiphora leptophloeos, as well as regarding the ability of extracts to interfere in biofilm formation, indicating their potential in the use for ruminant mastitis therapy. Keywords: Mastitis, Staphylococcus spp., imburana de cambão, phytotherapy, biofilm.https://www.revistas.ufg.br/vet/article/view/57228 |
| spellingShingle | Isamara Ferreira da Silva Amanda Leite Guimarães Vinícius Santana Amorim Telma Maria Guedes da Silva Rodolfo de Moraes Peixoto Xirley Pereira Nunes Tânia Maria Sarmento Silva Mateus Matiuzzi da Costa ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS Ciência Animal Brasileira |
| title | ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS |
| title_full | ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS |
| title_fullStr | ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS |
| title_full_unstemmed | ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS |
| title_short | ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS |
| title_sort | antimicrobial activity of ethanolic extracts from commiphora leptophloeos mart j b gillett against staphylococcus spp isolated from cases of mastitis in ruminants |
| url | https://www.revistas.ufg.br/vet/article/view/57228 |
| work_keys_str_mv | AT isamaraferreiradasilva antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT amandaleiteguimaraes antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT viniciussantanaamorim antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT telmamariaguedesdasilva antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT rodolfodemoraespeixoto antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT xirleypereiranunes antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT taniamariasarmentosilva antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants AT mateusmatiuzzidacosta antimicrobialactivityofethanolicextractsfromcommiphoraleptophloeosmartjbgillettagainststaphylococcussppisolatedfromcasesofmastitisinruminants |