A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil
A gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool s...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2016-06-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600403&lng=en&tlng=en |
| _version_ | 1797756603108163584 |
|---|---|
| author | Silvana Augusta Rodrigues Portes Eduardo de Mello Volotão Monica Simões Rocha Maria Cristina Rebelo Maria da Penha Trindade Pinheiro Xavier Rosane Maria de Assis Tatiana Lundgren Rose Marize Pereira Miagostovich José Paulo Gagliardi Leite Filipe Anibal Carvalho-Costa |
| author_facet | Silvana Augusta Rodrigues Portes Eduardo de Mello Volotão Monica Simões Rocha Maria Cristina Rebelo Maria da Penha Trindade Pinheiro Xavier Rosane Maria de Assis Tatiana Lundgren Rose Marize Pereira Miagostovich José Paulo Gagliardi Leite Filipe Anibal Carvalho-Costa |
| author_sort | Silvana Augusta Rodrigues Portes |
| collection | DOAJ |
| description | A gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed. |
| first_indexed | 2024-03-12T18:03:56Z |
| format | Article |
| id | doaj.art-ecf4acc0ad434c1f9aaaf6cc87d6b49e |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:03:56Z |
| publishDate | 2016-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-ecf4acc0ad434c1f9aaaf6cc87d6b49e2023-08-02T09:32:28ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602016-06-01111640340610.1590/0074-02760160030S0074-02762016000600403A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, BrazilSilvana Augusta Rodrigues PortesEduardo de Mello VolotãoMonica Simões RochaMaria Cristina RebeloMaria da Penha Trindade Pinheiro XavierRosane Maria de AssisTatiana Lundgren RoseMarize Pereira MiagostovichJosé Paulo Gagliardi LeiteFilipe Anibal Carvalho-CostaA gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600403&lng=en&tlng=engastroenteritisadenovirusquantitative polymerase chain reaction |
| spellingShingle | Silvana Augusta Rodrigues Portes Eduardo de Mello Volotão Monica Simões Rocha Maria Cristina Rebelo Maria da Penha Trindade Pinheiro Xavier Rosane Maria de Assis Tatiana Lundgren Rose Marize Pereira Miagostovich José Paulo Gagliardi Leite Filipe Anibal Carvalho-Costa A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil Memorias do Instituto Oswaldo Cruz gastroenteritis adenovirus quantitative polymerase chain reaction |
| title | A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil |
| title_full | A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil |
| title_fullStr | A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil |
| title_full_unstemmed | A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil |
| title_short | A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil |
| title_sort | non enteric adenovirus a12 gastroenteritis outbreak in rio de janeiro brazil |
| topic | gastroenteritis adenovirus quantitative polymerase chain reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600403&lng=en&tlng=en |
| work_keys_str_mv | AT silvanaaugustarodriguesportes anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT eduardodemellovolotao anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT monicasimoesrocha anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT mariacristinarebelo anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT mariadapenhatrindadepinheiroxavier anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT rosanemariadeassis anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT tatianalundgrenrose anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT marizepereiramiagostovich anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT josepaulogagliardileite anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT filipeanibalcarvalhocosta anonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT silvanaaugustarodriguesportes nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT eduardodemellovolotao nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT monicasimoesrocha nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT mariacristinarebelo nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT mariadapenhatrindadepinheiroxavier nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT rosanemariadeassis nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT tatianalundgrenrose nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT marizepereiramiagostovich nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT josepaulogagliardileite nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil AT filipeanibalcarvalhocosta nonentericadenovirusa12gastroenteritisoutbreakinriodejaneirobrazil |