Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis
Summary: Adenomyosis is a burdensome gynecologic condition that is associated with pelvic pain, dysmenorrhea, and abnormal uterine bleeding, leading to a negative impact on quality of life; and yet is often left undiagnosed. We recruited 108 women undergoing hysterectomy for benign gynecologic condi...
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| Format: | Artikel |
| Sprache: | English |
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Elsevier
2022-12-01
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| Schriftenreihe: | iScience |
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| Online Zugang: | http://www.sciencedirect.com/science/article/pii/S2589004222017801 |
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| author | Georgia M. Lorentzen Paweł Łaniewski Haiyan Cui Denise J. Roe Jamal Mourad Nichole D. Mahnert Leslie V. Farland Melissa M. Herbst-Kralovetz |
| author_facet | Georgia M. Lorentzen Paweł Łaniewski Haiyan Cui Denise J. Roe Jamal Mourad Nichole D. Mahnert Leslie V. Farland Melissa M. Herbst-Kralovetz |
| author_sort | Georgia M. Lorentzen |
| collection | DOAJ |
| description | Summary: Adenomyosis is a burdensome gynecologic condition that is associated with pelvic pain, dysmenorrhea, and abnormal uterine bleeding, leading to a negative impact on quality of life; and yet is often left undiagnosed. We recruited 108 women undergoing hysterectomy for benign gynecologic conditions and collected non-invasive cervicovaginal lavage samples for immunometabolic profiling. Patients were grouped according to adenomyosis status. We investigated the levels of 72 soluble immune proteins and >900 metabolites using multiplex immunoassays and an untargeted global metabolomics platform. There were statistically significant alterations in the levels of several immune proteins and a large quantity of metabolites, particularly cytokines related to type II immunity and amino acids, respectively. Enrichment analysis revealed that pyrimidine metabolism, carnitine synthesis, and histidine/histamine metabolism were significantly upregulated pathways in adenomyosis. This study demonstrates utility of non-invasive sampling combined with immunometabolic profiling for adenomyosis detection and a greater pathophysiological understanding of this enigmatic condition. |
| first_indexed | 2024-04-11T06:52:56Z |
| format | Article |
| id | doaj.art-ecf4cacadf934df9ae69cf0d05df4e2d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T06:52:56Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ecf4cacadf934df9ae69cf0d05df4e2d2022-12-22T04:39:08ZengElsevieriScience2589-00422022-12-012512105508Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosisGeorgia M. Lorentzen0Paweł Łaniewski1Haiyan Cui2Denise J. Roe3Jamal Mourad4Nichole D. Mahnert5Leslie V. Farland6Melissa M. Herbst-Kralovetz7Department of Obstetrics and Gynaecology, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USA; Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UKDepartment of Basic Medical Sciences, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USAUA Cancer Center, University of Arizona, Tucson, AZ 85719, USAUA Cancer Center, University of Arizona, Tucson, AZ 85719, USA; Department of Epidemiology and Biostatistics, Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, AZ 85724, USADepartment of Obstetrics and Gynaecology, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USA; Department of Obstetrics and Gynaecology, Banner-University Medical Center Phoenix, Phoenix, AZ 85006, USADepartment of Obstetrics and Gynaecology, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USA; Department of Obstetrics and Gynaecology, Banner-University Medical Center Phoenix, Phoenix, AZ 85006, USADepartment of Epidemiology and Biostatistics, Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, AZ 85724, USA; Department of Obstetrics and Gynaecology, University of Arizona, College of Medicine Tucson, Tucson, AZ 85724, USADepartment of Obstetrics and Gynaecology, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USA; Department of Basic Medical Sciences, College of Medicine – Phoenix, University of Arizona, Phoenix, AZ 85004, USA; UA Cancer Center, University of Arizona, Tucson, AZ 85719, USA; Corresponding authorSummary: Adenomyosis is a burdensome gynecologic condition that is associated with pelvic pain, dysmenorrhea, and abnormal uterine bleeding, leading to a negative impact on quality of life; and yet is often left undiagnosed. We recruited 108 women undergoing hysterectomy for benign gynecologic conditions and collected non-invasive cervicovaginal lavage samples for immunometabolic profiling. Patients were grouped according to adenomyosis status. We investigated the levels of 72 soluble immune proteins and >900 metabolites using multiplex immunoassays and an untargeted global metabolomics platform. There were statistically significant alterations in the levels of several immune proteins and a large quantity of metabolites, particularly cytokines related to type II immunity and amino acids, respectively. Enrichment analysis revealed that pyrimidine metabolism, carnitine synthesis, and histidine/histamine metabolism were significantly upregulated pathways in adenomyosis. This study demonstrates utility of non-invasive sampling combined with immunometabolic profiling for adenomyosis detection and a greater pathophysiological understanding of this enigmatic condition.http://www.sciencedirect.com/science/article/pii/S2589004222017801Health sciencesPathophysiologyImmunologyMetabolomics |
| spellingShingle | Georgia M. Lorentzen Paweł Łaniewski Haiyan Cui Denise J. Roe Jamal Mourad Nichole D. Mahnert Leslie V. Farland Melissa M. Herbst-Kralovetz Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis iScience Health sciences Pathophysiology Immunology Metabolomics |
| title | Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| title_full | Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| title_fullStr | Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| title_full_unstemmed | Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| title_short | Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| title_sort | immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis |
| topic | Health sciences Pathophysiology Immunology Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222017801 |
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