GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism
Arsenate (AsV) is one of the most hazardous elements, and its increasing accumulation in plants is the major area of concern in present days. Therefore, an environmentally reliable approach is required to reduce the load of AsV into vegetables. In the current study, the potential role of gamma amino...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23002051 |
| _version_ | 1797362577485856768 |
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| author | Mohammad Suhel Tajammul Husain Sheo Mohan Prasad Vijay Pratap Singh |
| author_facet | Mohammad Suhel Tajammul Husain Sheo Mohan Prasad Vijay Pratap Singh |
| author_sort | Mohammad Suhel |
| collection | DOAJ |
| description | Arsenate (AsV) is one of the most hazardous elements, and its increasing accumulation in plants is the major area of concern in present days. Therefore, an environmentally reliable approach is required to reduce the load of AsV into vegetables. In the current study, the potential role of gamma amino butyric acid (GABA; 10 µM) and sodium nitroprusside a donor of NO (SNP; 10 µM) in 21 days old seedlings of tomato and brinjal grown in Hoagland medium containing 25 µM of AsV was analysed by estimating physiological and biochemical attributes. AsV exposure reduced root and shoot length and dry mass by 18, 9 % and 15 % in tomato, and 23 and 10 %, and 27 % in brinjal seedlings, respectively. Additionally, AsV decreased leaf gas exchange parameters i.e. stomatal conductance, internal CO2 concentration, transpiration rate and subsequently photosynthetic rate. Although AsV stimulated sulphur assimilation and proline metabolism, but it raised the oxidative stress as evident by increased biomarkers; superoxide radicals (SOR), hydrogen peroxide (H2O2), lipid peroxidation (MDA), and protein oxidation in both the seedlings. The application of GABA and SNP significantly mitigated negative effects of AsV on both the vegetables. The results indicate that GABA was unable to reduce AsV stress in the presence of L-NAME (an endogenous NO biosynthetic inhibitor), but addition of SNP rescued the effect of L-NAME. Overall, the findings suggest that GABA and SNP effectively mitigate AsV stress however, the involvement of NO appears to be necessary for GABA to achieve this task efficiently in the studied vegetables. These results will help to design strategies for mitigating arsenate stress in vegetable crops. |
| first_indexed | 2024-03-08T16:09:54Z |
| format | Article |
| id | doaj.art-ecf892ab79464187b5b267ce9f953703 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-08T16:09:54Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-ecf892ab79464187b5b267ce9f9537032024-01-08T04:09:33ZengElsevierPlant Stress2667-064X2024-03-0111100338GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolismMohammad Suhel0Tajammul Husain1Sheo Mohan Prasad2Vijay Pratap Singh3Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj 211002, IndiaRanjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj 211002, IndiaRanjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj 211002, India; Corresponding author.Plant Physiology Laboratory, Department of Botany, C.M.P. Degree College, A Constituent Post Graduate College of University of Allahabad, Prayagraj 211002, IndiaArsenate (AsV) is one of the most hazardous elements, and its increasing accumulation in plants is the major area of concern in present days. Therefore, an environmentally reliable approach is required to reduce the load of AsV into vegetables. In the current study, the potential role of gamma amino butyric acid (GABA; 10 µM) and sodium nitroprusside a donor of NO (SNP; 10 µM) in 21 days old seedlings of tomato and brinjal grown in Hoagland medium containing 25 µM of AsV was analysed by estimating physiological and biochemical attributes. AsV exposure reduced root and shoot length and dry mass by 18, 9 % and 15 % in tomato, and 23 and 10 %, and 27 % in brinjal seedlings, respectively. Additionally, AsV decreased leaf gas exchange parameters i.e. stomatal conductance, internal CO2 concentration, transpiration rate and subsequently photosynthetic rate. Although AsV stimulated sulphur assimilation and proline metabolism, but it raised the oxidative stress as evident by increased biomarkers; superoxide radicals (SOR), hydrogen peroxide (H2O2), lipid peroxidation (MDA), and protein oxidation in both the seedlings. The application of GABA and SNP significantly mitigated negative effects of AsV on both the vegetables. The results indicate that GABA was unable to reduce AsV stress in the presence of L-NAME (an endogenous NO biosynthetic inhibitor), but addition of SNP rescued the effect of L-NAME. Overall, the findings suggest that GABA and SNP effectively mitigate AsV stress however, the involvement of NO appears to be necessary for GABA to achieve this task efficiently in the studied vegetables. These results will help to design strategies for mitigating arsenate stress in vegetable crops.http://www.sciencedirect.com/science/article/pii/S2667064X23002051GrowthOxidative biomarkersPhotosynthetic attributesStress alleviation |
| spellingShingle | Mohammad Suhel Tajammul Husain Sheo Mohan Prasad Vijay Pratap Singh GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism Plant Stress Growth Oxidative biomarkers Photosynthetic attributes Stress alleviation |
| title | GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| title_full | GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| title_fullStr | GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| title_full_unstemmed | GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| title_short | GABA and nitric oxide reduce AsV toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| title_sort | gaba and nitric oxide reduce asv toxicity in tomato and brinjal seedlings by regulating sulphur assimilation and proline metabolism |
| topic | Growth Oxidative biomarkers Photosynthetic attributes Stress alleviation |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23002051 |
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