Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice
14-3-3 proteins are present in all eukaryotic organisms and are ubiquitously expressed in a broad range of tissues and cellular compartments. They are regulatory adapter proteins that play key roles in a variety of signaling pathways, and have been proposed as suitable targets for the control and de...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/3/515 |
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| author | Anna Stachyra Sylwia Grzelak Katarzyna Basałaj Anna Zawistowska-Deniziak Justyna Bień-Kalinowska |
| author_facet | Anna Stachyra Sylwia Grzelak Katarzyna Basałaj Anna Zawistowska-Deniziak Justyna Bień-Kalinowska |
| author_sort | Anna Stachyra |
| collection | DOAJ |
| description | 14-3-3 proteins are present in all eukaryotic organisms and are ubiquitously expressed in a broad range of tissues and cellular compartments. They are regulatory adapter proteins that play key roles in a variety of signaling pathways, and have been proposed as suitable targets for the control and detection of certain parasites. <i>Trichinella britovi</i> is a widely-distributed parasitic nematode, transmitted through ingestion of meat products containing invasive larvae. The present study describes the cloning and expression of Tb14-3-3, and investigates the immunological and protective potential of the recombinant protein. Immunization of mice with rTb14-3-3 triggered an IgG response, and significant differences, in the profiles of secreted cytokines observed in vitro, between experimental groups. Nonetheless, neither specific antibodies, nor increased secretion of IFNγ, IL-4, and IL-10 cytokines, conferred greater protection against infection. No reduction in larval burden was observed during recovery at 48 dpi. Additionally, rTb14-3-3 was not recognized by sera from the infected control mice, except for one, suggesting some mismatch between native and recombinant Tb14-3-3 antigenic sites. Therefore, before 14-3-3 can be considered a potential tool for <i>Trichinella</i> detection and vaccination, more research regarding its target proteins, and actual specific function, is needed. |
| first_indexed | 2024-03-10T16:28:05Z |
| format | Article |
| id | doaj.art-ecf8a1963d7c43728985004f1e955934 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T16:28:05Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-ecf8a1963d7c43728985004f1e9559342023-11-20T13:04:20ZengMDPI AGVaccines2076-393X2020-09-018351510.3390/vaccines8030515Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in MiceAnna Stachyra0Sylwia Grzelak1Katarzyna Basałaj2Anna Zawistowska-Deniziak3Justyna Bień-Kalinowska4Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, PolandWitold Stefański Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, PolandWitold Stefański Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, PolandWitold Stefański Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, PolandWitold Stefański Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, Poland14-3-3 proteins are present in all eukaryotic organisms and are ubiquitously expressed in a broad range of tissues and cellular compartments. They are regulatory adapter proteins that play key roles in a variety of signaling pathways, and have been proposed as suitable targets for the control and detection of certain parasites. <i>Trichinella britovi</i> is a widely-distributed parasitic nematode, transmitted through ingestion of meat products containing invasive larvae. The present study describes the cloning and expression of Tb14-3-3, and investigates the immunological and protective potential of the recombinant protein. Immunization of mice with rTb14-3-3 triggered an IgG response, and significant differences, in the profiles of secreted cytokines observed in vitro, between experimental groups. Nonetheless, neither specific antibodies, nor increased secretion of IFNγ, IL-4, and IL-10 cytokines, conferred greater protection against infection. No reduction in larval burden was observed during recovery at 48 dpi. Additionally, rTb14-3-3 was not recognized by sera from the infected control mice, except for one, suggesting some mismatch between native and recombinant Tb14-3-3 antigenic sites. Therefore, before 14-3-3 can be considered a potential tool for <i>Trichinella</i> detection and vaccination, more research regarding its target proteins, and actual specific function, is needed.https://www.mdpi.com/2076-393X/8/3/515<i>Trichinella britovi</i>14-3-3 proteinimmune responsecytokinesimmunization |
| spellingShingle | Anna Stachyra Sylwia Grzelak Katarzyna Basałaj Anna Zawistowska-Deniziak Justyna Bień-Kalinowska Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice Vaccines <i>Trichinella britovi</i> 14-3-3 protein immune response cytokines immunization |
| title | Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice |
| title_full | Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice |
| title_fullStr | Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice |
| title_full_unstemmed | Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice |
| title_short | Immunization with a Recombinant Protein of <em>Trichinella britovi</em> 14-3-3 Triggers an Immune Response but No Protection in Mice |
| title_sort | immunization with a recombinant protein of em trichinella britovi em 14 3 3 triggers an immune response but no protection in mice |
| topic | <i>Trichinella britovi</i> 14-3-3 protein immune response cytokines immunization |
| url | https://www.mdpi.com/2076-393X/8/3/515 |
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