BoardION: real-time monitoring of Oxford Nanopore sequencing instruments
Abstract Background One of the main advantages of the Oxford Nanopore Technology (ONT) is the possibility of real-time sequencing. This gives access to information during the experiment and allows either to control the sequencing or to stop the sequencing once the results have been obtained. However...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | BMC Bioinformatics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12859-021-04161-0 |
| _version_ | 1829134637738229760 |
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| author | Aimeric Bruno Jean-Marc Aury Stefan Engelen |
| author_facet | Aimeric Bruno Jean-Marc Aury Stefan Engelen |
| author_sort | Aimeric Bruno |
| collection | DOAJ |
| description | Abstract Background One of the main advantages of the Oxford Nanopore Technology (ONT) is the possibility of real-time sequencing. This gives access to information during the experiment and allows either to control the sequencing or to stop the sequencing once the results have been obtained. However, the ONT sequencing interface is not sufficient to explore the quality of sequencing data in depth and existing quality control tools do not take full advantage of real-time data streaming. Results Herein, we present BoardION, an interactive web application to analyze the efficiency of ONT sequencing runs. The interactive interface of BoardION allows users to easily explore sequencing metrics and optimize the quantity and the quality of the data generated during the experiment. It also enables the comparison of multiple flowcells to assess library preparation protocols or the quality of input samples. Conclusion BoardION is dedicated to people who manage ONT sequencing instruments and allows them to remotely and in real time monitor their experiments and compare multiple sequencing runs. Source code, a Docker image and a demo version are available at http://www.genoscope.cns.fr/boardion/ . |
| first_indexed | 2024-12-14T18:08:08Z |
| format | Article |
| id | doaj.art-ecf90eb929bc422698b29a1e23e5fda2 |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-12-14T18:08:08Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj.art-ecf90eb929bc422698b29a1e23e5fda22022-12-21T22:52:20ZengBMCBMC Bioinformatics1471-21052021-05-012211810.1186/s12859-021-04161-0BoardION: real-time monitoring of Oxford Nanopore sequencing instrumentsAimeric Bruno0Jean-Marc Aury1Stefan Engelen2Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-SaclayGénomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-SaclayGénomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-SaclayAbstract Background One of the main advantages of the Oxford Nanopore Technology (ONT) is the possibility of real-time sequencing. This gives access to information during the experiment and allows either to control the sequencing or to stop the sequencing once the results have been obtained. However, the ONT sequencing interface is not sufficient to explore the quality of sequencing data in depth and existing quality control tools do not take full advantage of real-time data streaming. Results Herein, we present BoardION, an interactive web application to analyze the efficiency of ONT sequencing runs. The interactive interface of BoardION allows users to easily explore sequencing metrics and optimize the quantity and the quality of the data generated during the experiment. It also enables the comparison of multiple flowcells to assess library preparation protocols or the quality of input samples. Conclusion BoardION is dedicated to people who manage ONT sequencing instruments and allows them to remotely and in real time monitor their experiments and compare multiple sequencing runs. Source code, a Docker image and a demo version are available at http://www.genoscope.cns.fr/boardion/ .https://doi.org/10.1186/s12859-021-04161-0Nanopore sequencingReal-time monitoringWeb application |
| spellingShingle | Aimeric Bruno Jean-Marc Aury Stefan Engelen BoardION: real-time monitoring of Oxford Nanopore sequencing instruments BMC Bioinformatics Nanopore sequencing Real-time monitoring Web application |
| title | BoardION: real-time monitoring of Oxford Nanopore sequencing instruments |
| title_full | BoardION: real-time monitoring of Oxford Nanopore sequencing instruments |
| title_fullStr | BoardION: real-time monitoring of Oxford Nanopore sequencing instruments |
| title_full_unstemmed | BoardION: real-time monitoring of Oxford Nanopore sequencing instruments |
| title_short | BoardION: real-time monitoring of Oxford Nanopore sequencing instruments |
| title_sort | boardion real time monitoring of oxford nanopore sequencing instruments |
| topic | Nanopore sequencing Real-time monitoring Web application |
| url | https://doi.org/10.1186/s12859-021-04161-0 |
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