Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix)
Silver carp (Hypophthalmichthys molitrix) is a widely distributed food resource and phytoplankton predator in China, which has been used to control algal blooms. Silver carp is quite resistant to microcystins (MCs), which are typical hepatotoxins generated by toxic algae. ATP-Binding Cassette, Subfa...
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| Format: | Article |
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Elsevier
2023-06-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300131X |
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| author | Xiaohui Li Cui Feng Hang Sha Xiangzhong Luo Liguo Huang Guiwei Zou Hongwei Liang |
| author_facet | Xiaohui Li Cui Feng Hang Sha Xiangzhong Luo Liguo Huang Guiwei Zou Hongwei Liang |
| author_sort | Xiaohui Li |
| collection | DOAJ |
| description | Silver carp (Hypophthalmichthys molitrix) is a widely distributed food resource and phytoplankton predator in China, which has been used to control algal blooms. Silver carp is quite resistant to microcystins (MCs), which are typical hepatotoxins generated by toxic algae. ATP-Binding Cassette, Subfamily C (ABCC) transporter are critical detoxification genes that could export toxic substances out of the cell, and with regard to ABCC transporters in H. molitrix, current studies are limited. In order to investigate the roles of ABCC genes in excretion of MCs, the full-length transcriptome of silver carp was analyzed to screen a total of nine ABCC transporter genes, which were expressed ubiquitously in silver carp tissues, especially in the liver. Furthermore, microcystin-LR (MC-LR) intraperitoneal injection was performed, and the results showed that, except abcc2, abcc10 and abcc12, the expression of other ABCC genes increased rapidly within 1 h and then began to decline after 6 h. Among these five genes, abcc3 and abcc9 exhibited the most pronounced response, indicating that these two were the most preeminent detoxification genes of microcystin in ABCC genes family in silver carp. Meanwhile, the activities of glutathione-S-transferase (GST) were increased significantly, while the content of glutathione (GSH) demonstrated a dramatic decrease, implying that GST catalyzed binding of GSH and MC-LR, and then excreted through ABCC transporters. The findings of this study will be useful in further investigating the fundamental characteristics of silver carp and provide new insights into the potential function of ABCC genes in the detoxication of MC-LR. |
| first_indexed | 2024-03-13T04:56:06Z |
| format | Article |
| id | doaj.art-ecfb7a3b953c48968e71997766590ae1 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-13T04:56:06Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ecfb7a3b953c48968e71997766590ae12023-06-18T05:02:28ZengElsevierAquaculture Reports2352-51342023-06-0130101592Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix)Xiaohui Li0Cui Feng1Hang Sha2Xiangzhong Luo3Liguo Huang4Guiwei Zou5Hongwei Liang6Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Freshwater Aquaculture genetic and breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaNational Original Seed Farm of Yangtze River Four Major Chinese Carps in Laohe, Shishou, Jingzhou 434405, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Corresponding author.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author at: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.Silver carp (Hypophthalmichthys molitrix) is a widely distributed food resource and phytoplankton predator in China, which has been used to control algal blooms. Silver carp is quite resistant to microcystins (MCs), which are typical hepatotoxins generated by toxic algae. ATP-Binding Cassette, Subfamily C (ABCC) transporter are critical detoxification genes that could export toxic substances out of the cell, and with regard to ABCC transporters in H. molitrix, current studies are limited. In order to investigate the roles of ABCC genes in excretion of MCs, the full-length transcriptome of silver carp was analyzed to screen a total of nine ABCC transporter genes, which were expressed ubiquitously in silver carp tissues, especially in the liver. Furthermore, microcystin-LR (MC-LR) intraperitoneal injection was performed, and the results showed that, except abcc2, abcc10 and abcc12, the expression of other ABCC genes increased rapidly within 1 h and then began to decline after 6 h. Among these five genes, abcc3 and abcc9 exhibited the most pronounced response, indicating that these two were the most preeminent detoxification genes of microcystin in ABCC genes family in silver carp. Meanwhile, the activities of glutathione-S-transferase (GST) were increased significantly, while the content of glutathione (GSH) demonstrated a dramatic decrease, implying that GST catalyzed binding of GSH and MC-LR, and then excreted through ABCC transporters. The findings of this study will be useful in further investigating the fundamental characteristics of silver carp and provide new insights into the potential function of ABCC genes in the detoxication of MC-LR.http://www.sciencedirect.com/science/article/pii/S235251342300131XHypophthalmichthys molitrixSMRT sequencingABCCMC-LR |
| spellingShingle | Xiaohui Li Cui Feng Hang Sha Xiangzhong Luo Liguo Huang Guiwei Zou Hongwei Liang Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) Aquaculture Reports Hypophthalmichthys molitrix SMRT sequencing ABCC MC-LR |
| title | Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) |
| title_full | Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) |
| title_fullStr | Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) |
| title_full_unstemmed | Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) |
| title_short | Identification and characterization of ABCC gene family and their roles in the response to intraperitoneal injection of microcystin-LR in liver of silver carp (Hypophthalmichthys molitrix) |
| title_sort | identification and characterization of abcc gene family and their roles in the response to intraperitoneal injection of microcystin lr in liver of silver carp hypophthalmichthys molitrix |
| topic | Hypophthalmichthys molitrix SMRT sequencing ABCC MC-LR |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300131X |
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