Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver
<i>Premna odorata</i> Blanco (Lamiaceae) is an ethnomedicinal plant native to different tropical regions. Although some reports addressed their anti-inflammatory, cytotoxic, and antituberculotic effects, their hepatoprotective potential is yet to be discovered. Accordingly, this study in...
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MDPI AG
2020-07-01
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| author | Abeer H. Elmaidomy Hani A. Alhadrami Elham Amin Hanan F. Aly Asmaa M. Othman Mostafa E. Rateb Mona H. Hetta Usama Ramadan Abdelmohsen Hossam M. Hassan |
| author_facet | Abeer H. Elmaidomy Hani A. Alhadrami Elham Amin Hanan F. Aly Asmaa M. Othman Mostafa E. Rateb Mona H. Hetta Usama Ramadan Abdelmohsen Hossam M. Hassan |
| author_sort | Abeer H. Elmaidomy |
| collection | DOAJ |
| description | <i>Premna odorata</i> Blanco (Lamiaceae) is an ethnomedicinal plant native to different tropical regions. Although some reports addressed their anti-inflammatory, cytotoxic, and antituberculotic effects, their hepatoprotective potential is yet to be discovered. Accordingly, this study investigated the crude extract and different fractions of the plant leaves; metabolic profiling using liquid chromatography/high-resolution electrospray ionization mass spectroscopy (LC–HRESIMS) analysis, <i>in silico</i> absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties for the dereplicated metabolite via online PreADMET program, ROS scavenger activity on the Hep G2 human liver cancer cell line, and the possible hepatic cellular treatment effects in alcohol-inflamed liver female Wistar albino rats. Metabolic profiling dereplicated a total of 28 metabolites from the crude extract and its various fractions. In silico ADMET and ROS scavenger activity screening suggested plant metabolites are of potential bioactivity. In vivo hepatic treatment with crude, defatted crude, and n-hexane leave extracts suggested all extracts significantly improved liver damage, which was indicated by the reduction of elevated serum levels of bilirubin, AST, ALT, ALP, CRP, TNF-α, ICAM-1, VCAM-1, and MDA. The reduced levels of GSH and TAC were normalized during the study. Histological examinations of liver tissue showed collagen fiber distribution nearly back to its normal pattern. The anti-inflammatory and antioxidant potentials of <i>Premna odorata</i> extracts could be partly related to the combined effects of these phytochemicals or their synergistic interactions. |
| first_indexed | 2024-03-10T18:37:03Z |
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| last_indexed | 2024-03-10T18:37:03Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ecfe68dbc1b6445fa852a8ad708617ba2023-11-20T06:10:49ZengMDPI AGMolecules1420-30492020-07-012514311610.3390/molecules25143116Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat LiverAbeer H. Elmaidomy0Hani A. Alhadrami1Elham Amin2Hanan F. Aly3Asmaa M. Othman4Mostafa E. Rateb5Mona H. Hetta6Usama Ramadan Abdelmohsen7Hossam M. Hassan8Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80402 Jeddah 21589, Saudi ArabiDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptTherapeutic Chemistry Department, National Research Centre, Cairo 11865, EgyptDepartment of Internal Medicine, Faculty of Medicine, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Fayoum University, Fayoum 63514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt<i>Premna odorata</i> Blanco (Lamiaceae) is an ethnomedicinal plant native to different tropical regions. Although some reports addressed their anti-inflammatory, cytotoxic, and antituberculotic effects, their hepatoprotective potential is yet to be discovered. Accordingly, this study investigated the crude extract and different fractions of the plant leaves; metabolic profiling using liquid chromatography/high-resolution electrospray ionization mass spectroscopy (LC–HRESIMS) analysis, <i>in silico</i> absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties for the dereplicated metabolite via online PreADMET program, ROS scavenger activity on the Hep G2 human liver cancer cell line, and the possible hepatic cellular treatment effects in alcohol-inflamed liver female Wistar albino rats. Metabolic profiling dereplicated a total of 28 metabolites from the crude extract and its various fractions. In silico ADMET and ROS scavenger activity screening suggested plant metabolites are of potential bioactivity. In vivo hepatic treatment with crude, defatted crude, and n-hexane leave extracts suggested all extracts significantly improved liver damage, which was indicated by the reduction of elevated serum levels of bilirubin, AST, ALT, ALP, CRP, TNF-α, ICAM-1, VCAM-1, and MDA. The reduced levels of GSH and TAC were normalized during the study. Histological examinations of liver tissue showed collagen fiber distribution nearly back to its normal pattern. The anti-inflammatory and antioxidant potentials of <i>Premna odorata</i> extracts could be partly related to the combined effects of these phytochemicals or their synergistic interactions.https://www.mdpi.com/1420-3049/25/14/3116<i>Premna odorata</i>LC–HRESIMSterpenepolyphenolicADMETROS |
| spellingShingle | Abeer H. Elmaidomy Hani A. Alhadrami Elham Amin Hanan F. Aly Asmaa M. Othman Mostafa E. Rateb Mona H. Hetta Usama Ramadan Abdelmohsen Hossam M. Hassan Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver Molecules <i>Premna odorata</i> LC–HRESIMS terpene polyphenolic ADMET ROS |
| title | Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver |
| title_full | Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver |
| title_fullStr | Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver |
| title_full_unstemmed | Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver |
| title_short | Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich <i>Premna odorata</i> Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver |
| title_sort | anti inflammatory and antioxidant activities of terpene and polyphenol rich i premna odorata i leaves on alcohol inflamed female wistar albino rat liver |
| topic | <i>Premna odorata</i> LC–HRESIMS terpene polyphenolic ADMET ROS |
| url | https://www.mdpi.com/1420-3049/25/14/3116 |
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