A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring
The long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as <i>Escherichia coli</i>, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/1/16 |
| _version_ | 1819209204987068416 |
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| author | Keisha Y. Castillo-Torres Eric S. McLamore David P. Arnold |
| author_facet | Keisha Y. Castillo-Torres Eric S. McLamore David P. Arnold |
| author_sort | Keisha Y. Castillo-Torres |
| collection | DOAJ |
| description | The long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as <i>Escherichia coli</i>, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for magnetic separation microfluidic analysis platforms that typically function with <20 µL/s throughput. Here, we report the use of 1.5-µm-diameter magnetic microdisc to selectively tag target bacteria, and a high-throughput microfluidic device that can potentially isolate the magnetically tagged bacteria from 100 mL water samples in less than 15 min. Simulations and experiments show ~90% capture efficiencies of magnetic particles at flow rates up to 120 µL/s. Also, the platform enables the magnetic microdiscs/bacteria conjugates to be directly imaged, providing a path for quantitative assay. |
| first_indexed | 2024-12-23T05:51:34Z |
| format | Article |
| id | doaj.art-ed05cc3f9a2147af999e534e247efef8 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-23T05:51:34Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-ed05cc3f9a2147af999e534e247efef82022-12-21T17:57:56ZengMDPI AGMicromachines2072-666X2019-12-011111610.3390/mi11010016mi11010016A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality MonitoringKeisha Y. Castillo-Torres0Eric S. McLamore1David P. Arnold2Interdisciplinary Microsystems Group, Department of Electrical and Computer Engineering; University of Florida, Gainesville, FL 32611, USAInstitute of Food and Agricultural Sciences, Department of Agricultural and Biological Engineering; University of Florida, Gainesville, FL 32611, USAInterdisciplinary Microsystems Group, Department of Electrical and Computer Engineering; University of Florida, Gainesville, FL 32611, USAThe long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as <i>Escherichia coli</i>, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for magnetic separation microfluidic analysis platforms that typically function with <20 µL/s throughput. Here, we report the use of 1.5-µm-diameter magnetic microdisc to selectively tag target bacteria, and a high-throughput microfluidic device that can potentially isolate the magnetically tagged bacteria from 100 mL water samples in less than 15 min. Simulations and experiments show ~90% capture efficiencies of magnetic particles at flow rates up to 120 µL/s. Also, the platform enables the magnetic microdiscs/bacteria conjugates to be directly imaged, providing a path for quantitative assay.https://www.mdpi.com/2072-666X/11/1/16high-throughputmagnetic isolationmagnetic separationmagnetic microdiscsmicrofluidicsbacteria<i>escherichia coli</i>water quality |
| spellingShingle | Keisha Y. Castillo-Torres Eric S. McLamore David P. Arnold A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring Micromachines high-throughput magnetic isolation magnetic separation magnetic microdiscs microfluidics bacteria <i>escherichia coli</i> water quality |
| title | A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring |
| title_full | A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring |
| title_fullStr | A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring |
| title_full_unstemmed | A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring |
| title_short | A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring |
| title_sort | high throughput microfluidic magnetic separation µfms platform for water quality monitoring |
| topic | high-throughput magnetic isolation magnetic separation magnetic microdiscs microfluidics bacteria <i>escherichia coli</i> water quality |
| url | https://www.mdpi.com/2072-666X/11/1/16 |
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