Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs
Campylobacteriosis seems to be a growing problem worldwide. Apart from the most common sources of numerous <i>Campylobacter</i> species, such as poultry and other farm animals, dogs may be an underrated reservoir of this pathogen. Our goal was to establish the frequency of occurrence, an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/7/964 |
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| author | Małgorzata Murawska Monika Sypecka Justyna Bartosik Ewelina Kwiecień Magdalena Rzewuska Agnieszka Sałamaszyńska-Guz |
| author_facet | Małgorzata Murawska Monika Sypecka Justyna Bartosik Ewelina Kwiecień Magdalena Rzewuska Agnieszka Sałamaszyńska-Guz |
| author_sort | Małgorzata Murawska |
| collection | DOAJ |
| description | Campylobacteriosis seems to be a growing problem worldwide. Apart from the most common sources of numerous <i>Campylobacter</i> species, such as poultry and other farm animals, dogs may be an underrated reservoir of this pathogen. Our goal was to establish the frequency of occurrence, antimicrobial resistance, and detection of chosen virulence factor genes in genomes of canine <i>Campylobacter</i> isolates. <i>Campylobacter</i> isolates frequency in dogs from shelters, and private origin was 13%. All of the tested virulence factor genes were found in 28 of 31 isolates. We determined high resistance levels to the ciprofloxacin and ampicillin and moderate tetracycline resistance. For <i>C. jejuni</i> shelter isolates, genetic diversity was also determined using PFGE. Our results indicate that dogs may be the reservoir of potentially diverse, potentially virulent, and antimicrobial-resistant <i>Campylobacter</i> strains. |
| first_indexed | 2024-03-09T03:46:56Z |
| format | Article |
| id | doaj.art-ed07bb9ee7824f86aff12d3fd10bc332 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T03:46:56Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ed07bb9ee7824f86aff12d3fd10bc3322023-12-03T14:33:05ZengMDPI AGAntibiotics2079-63822022-07-0111796410.3390/antibiotics11070964Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian DogsMałgorzata Murawska0Monika Sypecka1Justyna Bartosik2Ewelina Kwiecień3Magdalena Rzewuska4Agnieszka Sałamaszyńska-Guz5Division of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandTranslational Platform for Regenerative Medicine, Mossakowski Medical Research Institute, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, PolandDivision of Parasitology and Invasive Diseases, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandCampylobacteriosis seems to be a growing problem worldwide. Apart from the most common sources of numerous <i>Campylobacter</i> species, such as poultry and other farm animals, dogs may be an underrated reservoir of this pathogen. Our goal was to establish the frequency of occurrence, antimicrobial resistance, and detection of chosen virulence factor genes in genomes of canine <i>Campylobacter</i> isolates. <i>Campylobacter</i> isolates frequency in dogs from shelters, and private origin was 13%. All of the tested virulence factor genes were found in 28 of 31 isolates. We determined high resistance levels to the ciprofloxacin and ampicillin and moderate tetracycline resistance. For <i>C. jejuni</i> shelter isolates, genetic diversity was also determined using PFGE. Our results indicate that dogs may be the reservoir of potentially diverse, potentially virulent, and antimicrobial-resistant <i>Campylobacter</i> strains.https://www.mdpi.com/2079-6382/11/7/964<i>Campylobacter jejuni</i>antimicrobial resistancevirulence factorsPFGEdogs |
| spellingShingle | Małgorzata Murawska Monika Sypecka Justyna Bartosik Ewelina Kwiecień Magdalena Rzewuska Agnieszka Sałamaszyńska-Guz Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs Antibiotics <i>Campylobacter jejuni</i> antimicrobial resistance virulence factors PFGE dogs |
| title | Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs |
| title_full | Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs |
| title_fullStr | Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs |
| title_full_unstemmed | Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs |
| title_short | Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity of <i>Campylobacter</i> spp. Isolated from Varsovian Dogs |
| title_sort | should we consider them as a threat antimicrobial resistance virulence potential and genetic diversity of i campylobacter i spp isolated from varsovian dogs |
| topic | <i>Campylobacter jejuni</i> antimicrobial resistance virulence factors PFGE dogs |
| url | https://www.mdpi.com/2079-6382/11/7/964 |
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