Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight
<i>Fusarium oxysporum</i>, a primary soil-borne fungus that affects rice seedlings globally, is responsible for rice seedling blight (RSB), which reduces seedling quality and survival rates. The synthetic fungicides used to treat this disease negatively affect human health and the enviro...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/1/167 |
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| author | Wanyi Jiang Jinxin Liu Ying He Ayihumaer Payizila Yonggang Li |
| author_facet | Wanyi Jiang Jinxin Liu Ying He Ayihumaer Payizila Yonggang Li |
| author_sort | Wanyi Jiang |
| collection | DOAJ |
| description | <i>Fusarium oxysporum</i>, a primary soil-borne fungus that affects rice seedlings globally, is responsible for rice seedling blight (RSB), which reduces seedling quality and survival rates. The synthetic fungicides used to treat this disease negatively affect human health and the environment. A biocontrol bacterial isolate, <i>Bacillus velezensis</i> Bv S3, isolated from the rice rhizosphere, showed a strong antagonistic effect on RSB-causing <i>F. oxysporum</i>. The ratio of the longest to the shortest radius of <i>F</i>. <i>oxysporum</i> following Bv S3 inoculation was 2.52 (cm/cm) in a plate standoff experiment. This was different from the other biocontrol strains. Bv S3 exhibits a wide spectrum of antifungal activity against various pathogenic fungi that cause RSB. When 10% Bv S3 liquid culture filtrate was applied, it dramatically reduced <i>F. oxysporum</i> spore germination and mycelial growth, with inhibition rates of 66.7%, and 45.7%, respectively, and caused hyphal malformations. Furthermore, the Bv S3 suspension (1 × 10<sup>8</sup> CFU/mL) reduced RSB by 65.5% and 76.5% in pot experiments, effectively promoted the growth of rice seedlings, and improved the activities of neutral phosphatase, urease, invertase, and catalase in rice rhizosphere soil. The active substances produced by Bv S3 were sensitive to temperature and ultraviolet irradiation, and the antifungal effect significantly increased after 90 min of exposure, with antifungal effect observed at pH 7. Bv. S3 effectively reduced the incidence of RSB and showed potential as a biocontrol agent. |
| first_indexed | 2024-03-08T11:08:56Z |
| format | Article |
| id | doaj.art-ed08a2a59c3449e286839e90c4c8ce0a |
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| language | English |
| last_indexed | 2024-03-08T11:08:56Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-ed08a2a59c3449e286839e90c4c8ce0a2024-01-26T14:26:19ZengMDPI AGAgronomy2073-43952024-01-0114116710.3390/agronomy14010167Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling BlightWanyi Jiang0Jinxin Liu1Ying He2Ayihumaer Payizila3Yonggang Li4College of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, China<i>Fusarium oxysporum</i>, a primary soil-borne fungus that affects rice seedlings globally, is responsible for rice seedling blight (RSB), which reduces seedling quality and survival rates. The synthetic fungicides used to treat this disease negatively affect human health and the environment. A biocontrol bacterial isolate, <i>Bacillus velezensis</i> Bv S3, isolated from the rice rhizosphere, showed a strong antagonistic effect on RSB-causing <i>F. oxysporum</i>. The ratio of the longest to the shortest radius of <i>F</i>. <i>oxysporum</i> following Bv S3 inoculation was 2.52 (cm/cm) in a plate standoff experiment. This was different from the other biocontrol strains. Bv S3 exhibits a wide spectrum of antifungal activity against various pathogenic fungi that cause RSB. When 10% Bv S3 liquid culture filtrate was applied, it dramatically reduced <i>F. oxysporum</i> spore germination and mycelial growth, with inhibition rates of 66.7%, and 45.7%, respectively, and caused hyphal malformations. Furthermore, the Bv S3 suspension (1 × 10<sup>8</sup> CFU/mL) reduced RSB by 65.5% and 76.5% in pot experiments, effectively promoted the growth of rice seedlings, and improved the activities of neutral phosphatase, urease, invertase, and catalase in rice rhizosphere soil. The active substances produced by Bv S3 were sensitive to temperature and ultraviolet irradiation, and the antifungal effect significantly increased after 90 min of exposure, with antifungal effect observed at pH 7. Bv. S3 effectively reduced the incidence of RSB and showed potential as a biocontrol agent.https://www.mdpi.com/2073-4395/14/1/167rice seedling blight<i>Bacillus velezensis</i>antifungal activitiesbiological controlsoil enzyme activity |
| spellingShingle | Wanyi Jiang Jinxin Liu Ying He Ayihumaer Payizila Yonggang Li Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight Agronomy rice seedling blight <i>Bacillus velezensis</i> antifungal activities biological control soil enzyme activity |
| title | Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight |
| title_full | Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight |
| title_fullStr | Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight |
| title_full_unstemmed | Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight |
| title_short | Biological Control Ability and Antifungal Activities of <i>Bacillus velezensis</i> Bv S3 against <i>Fusarium oxysporum</i> That Causes Rice Seedling Blight |
| title_sort | biological control ability and antifungal activities of i bacillus velezensis i bv s3 against i fusarium oxysporum i that causes rice seedling blight |
| topic | rice seedling blight <i>Bacillus velezensis</i> antifungal activities biological control soil enzyme activity |
| url | https://www.mdpi.com/2073-4395/14/1/167 |
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