The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds
Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 5...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2011-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100010 |
| _version_ | 1818576973761347584 |
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| author | Chunyan Zhang Yun Liu Kunkun Huang Wenbing Zeng Deqing Xu Qunying Wen Liguo Yang |
| author_facet | Chunyan Zhang Yun Liu Kunkun Huang Wenbing Zeng Deqing Xu Qunying Wen Liguo Yang |
| author_sort | Chunyan Zhang |
| collection | DOAJ |
| description | Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p < 0.05). On combining the two loci, both Matou and Boer dams with ABCD genotype had the largest litter sizes when compared to the other genotypes (p < 0.05). When undergoing like superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds. |
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| format | Article |
| id | doaj.art-ed0b533cae5641b8ac2c3e2b56b078bd |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-16T06:22:32Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-ed0b533cae5641b8ac2c3e2b56b078bd2022-12-21T22:41:06ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-013414955The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breedsChunyan ZhangYun LiuKunkun HuangWenbing ZengDeqing XuQunying WenLiguo YangTwo active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p < 0.05). On combining the two loci, both Matou and Boer dams with ABCD genotype had the largest litter sizes when compared to the other genotypes (p < 0.05). When undergoing like superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100010DNA polymorphismgrowth hormone (GH) genelitter sizesuperovulation responsegoat |
| spellingShingle | Chunyan Zhang Yun Liu Kunkun Huang Wenbing Zeng Deqing Xu Qunying Wen Liguo Yang The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds Genetics and Molecular Biology DNA polymorphism growth hormone (GH) gene litter size superovulation response goat |
| title | The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds |
| title_full | The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds |
| title_fullStr | The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds |
| title_full_unstemmed | The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds |
| title_short | The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds |
| title_sort | association of two single nucleotide polymorphisms snps in growth hormone gh gene with litter size and superovulation response in goat breeds |
| topic | DNA polymorphism growth hormone (GH) gene litter size superovulation response goat |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100010 |
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