Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage
Abstract Background Understanding of the biochemical and morphological lesions associated with storage of equine blood is limited. Objective To demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR‐pR...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-03-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.17015 |
| _version_ | 1797263093855682560 |
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| author | Arianna Miglio Francesca Rocconi Valentina Cremonini Angelo D'Alessandro Julie A. Reisz Mark Maslanka Ian S. Lacroix Giorgio Tiscar Morena Di Tommaso Maria T. Antognoni |
| author_facet | Arianna Miglio Francesca Rocconi Valentina Cremonini Angelo D'Alessandro Julie A. Reisz Mark Maslanka Ian S. Lacroix Giorgio Tiscar Morena Di Tommaso Maria T. Antognoni |
| author_sort | Arianna Miglio |
| collection | DOAJ |
| description | Abstract Background Understanding of the biochemical and morphological lesions associated with storage of equine blood is limited. Objective To demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR‐pRBC) and non‐leukoreduced packed RBC (nLR‐pRBC). Animals Packed RBC units were obtained from 6 healthy blood donor horses enrolled in 2 blood banks. Methods Observational study. Whole blood was collected from each donor using transfusion bags with a LR filter. Leukoreduction pRBC and nLR‐pRBC units were obtained and stored at 4°C for up 42 days. Sterile weekly sampling was performed from each unit for analyses. Results Red blood cells and supernatants progressively accumulated lactate products while high‐energy phosphate compounds (adenosine triphosphate and 2,3‐Diphosphoglycerate) declined. Hypoxanthine, xanthine, and free fatty acids accumulated in stored RBC and supernatants. These lesions were exacerbated in non‐LR‐pRBC. Conclusion and Clinical Importance Leukoreduction has a beneficial effect on RBC energy and redox metabolism of equine pRBC and the onset and severity of the metabolic storage lesions RBC. |
| first_indexed | 2024-04-25T00:07:32Z |
| format | Article |
| id | doaj.art-ed0d0c8742984dc2b297cfda298c25d7 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-25T00:07:32Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-ed0d0c8742984dc2b297cfda298c25d72024-03-14T04:25:21ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762024-03-013821185119510.1111/jvim.17015Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storageArianna Miglio0Francesca Rocconi1Valentina Cremonini2Angelo D'Alessandro3Julie A. Reisz4Mark Maslanka5Ian S. Lacroix6Giorgio Tiscar7Morena Di Tommaso8Maria T. Antognoni9Department of Veterinary Medicine University of Perugia Perugia ItalyDepartment of Veterinary Medicine Veterinary University Hospital, University of Teramo Teramo ItalyDepartment of Veterinary Medicine University of Perugia Perugia ItalyDepartment of Biochemistry and Molecular Genetics University of Colorado Denver—Anschutz Medical Campus Aurora Colorado USADepartment of Biochemistry and Molecular Genetics University of Colorado Denver—Anschutz Medical Campus Aurora Colorado USADepartment of Biochemistry and Molecular Genetics University of Colorado Denver—Anschutz Medical Campus Aurora Colorado USADepartment of Biochemistry and Molecular Genetics University of Colorado Denver—Anschutz Medical Campus Aurora Colorado USADepartment of Veterinary Medicine Veterinary University Hospital, University of Teramo Teramo ItalyDepartment of Veterinary Medicine Veterinary University Hospital, University of Teramo Teramo ItalyDepartment of Veterinary Medicine University of Perugia Perugia ItalyAbstract Background Understanding of the biochemical and morphological lesions associated with storage of equine blood is limited. Objective To demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR‐pRBC) and non‐leukoreduced packed RBC (nLR‐pRBC). Animals Packed RBC units were obtained from 6 healthy blood donor horses enrolled in 2 blood banks. Methods Observational study. Whole blood was collected from each donor using transfusion bags with a LR filter. Leukoreduction pRBC and nLR‐pRBC units were obtained and stored at 4°C for up 42 days. Sterile weekly sampling was performed from each unit for analyses. Results Red blood cells and supernatants progressively accumulated lactate products while high‐energy phosphate compounds (adenosine triphosphate and 2,3‐Diphosphoglycerate) declined. Hypoxanthine, xanthine, and free fatty acids accumulated in stored RBC and supernatants. These lesions were exacerbated in non‐LR‐pRBC. Conclusion and Clinical Importance Leukoreduction has a beneficial effect on RBC energy and redox metabolism of equine pRBC and the onset and severity of the metabolic storage lesions RBC.https://doi.org/10.1111/jvim.17015blood transfusionhorseleukoreductionlipidomicsmetabolomicsstorage lesion |
| spellingShingle | Arianna Miglio Francesca Rocconi Valentina Cremonini Angelo D'Alessandro Julie A. Reisz Mark Maslanka Ian S. Lacroix Giorgio Tiscar Morena Di Tommaso Maria T. Antognoni Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage Journal of Veterinary Internal Medicine blood transfusion horse leukoreduction lipidomics metabolomics storage lesion |
| title | Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| title_full | Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| title_fullStr | Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| title_full_unstemmed | Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| title_short | Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| title_sort | effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage |
| topic | blood transfusion horse leukoreduction lipidomics metabolomics storage lesion |
| url | https://doi.org/10.1111/jvim.17015 |
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