Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae)
The takin lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiolog...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13597 |
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| author | Yue Xie Yijun Chen Lidan Wang Zhao Wang Pengchen Zhu Zun Hu Xiaobin Gu Ran He Jing Xu Bo Jing Xuerong Peng Guangyou Yang Xuan Zhou |
| author_facet | Yue Xie Yijun Chen Lidan Wang Zhao Wang Pengchen Zhu Zun Hu Xiaobin Gu Ran He Jing Xu Bo Jing Xuerong Peng Guangyou Yang Xuan Zhou |
| author_sort | Yue Xie |
| collection | DOAJ |
| description | The takin lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiology and evolutionary biology of <i>V. eleguneniensis</i>. Mitochondrial genomes (mtDNAs) can provide resources for investigations in these areas and, therefore, can assist with the surveillance and control of this lungworm. Herein, the complete mtDNA of <i>V. eleguneniensis</i> was sequenced and characterized with Illumina pipeline analyses. This circular genome (13,625 bp) encoded twelve protein-coding genes (PCGs), two rRNAs, and twenty-two tRNAs, with notable levels of AT and GC skews. Comparative genomics revealed a purifying selection among PCGs, with <i>cox1</i> and <i>nad6</i> having the lowest and the highest evolutionary rate, respectively. Genome-wide phylogenies showed a close relationship between <i>V. eleguneniensis</i> and <i>Protostrongylus rufescens</i> in Strongylida. Single gene (PCGs or rRNAs)-based phylogenies indicated that <i>cox1</i> and <i>nad5</i> genes shared the same family-level topology with that inferred from genomic datasets, suggesting that both genes could be suitable genetic markers for evolutionary and phylogenetic studies of Strongylida species. This was the first mtDNA of any member of the genus <i>Varestrongylus,</i> and its comprehensive molecular characterization represents a new resource for systematic, population genetic and evolutionary biological studies of <i>Varestrongylus</i> lungworms in wildlife. |
| first_indexed | 2024-03-09T19:00:08Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:00:08Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ed0f17eedf794253bf8366ba5cf10cd52023-11-24T05:10:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211359710.3390/ijms232113597Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae)Yue Xie0Yijun Chen1Lidan Wang2Zhao Wang3Pengchen Zhu4Zun Hu5Xiaobin Gu6Ran He7Jing Xu8Bo Jing9Xuerong Peng10Guangyou Yang11Xuan Zhou12Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe takin lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiology and evolutionary biology of <i>V. eleguneniensis</i>. Mitochondrial genomes (mtDNAs) can provide resources for investigations in these areas and, therefore, can assist with the surveillance and control of this lungworm. Herein, the complete mtDNA of <i>V. eleguneniensis</i> was sequenced and characterized with Illumina pipeline analyses. This circular genome (13,625 bp) encoded twelve protein-coding genes (PCGs), two rRNAs, and twenty-two tRNAs, with notable levels of AT and GC skews. Comparative genomics revealed a purifying selection among PCGs, with <i>cox1</i> and <i>nad6</i> having the lowest and the highest evolutionary rate, respectively. Genome-wide phylogenies showed a close relationship between <i>V. eleguneniensis</i> and <i>Protostrongylus rufescens</i> in Strongylida. Single gene (PCGs or rRNAs)-based phylogenies indicated that <i>cox1</i> and <i>nad5</i> genes shared the same family-level topology with that inferred from genomic datasets, suggesting that both genes could be suitable genetic markers for evolutionary and phylogenetic studies of Strongylida species. This was the first mtDNA of any member of the genus <i>Varestrongylus,</i> and its comprehensive molecular characterization represents a new resource for systematic, population genetic and evolutionary biological studies of <i>Varestrongylus</i> lungworms in wildlife.https://www.mdpi.com/1422-0067/23/21/13597<i>Varestrongylus</i> lungwormtakinsmitogenome |
| spellingShingle | Yue Xie Yijun Chen Lidan Wang Zhao Wang Pengchen Zhu Zun Hu Xiaobin Gu Ran He Jing Xu Bo Jing Xuerong Peng Guangyou Yang Xuan Zhou Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) International Journal of Molecular Sciences <i>Varestrongylus</i> lungworm takins mitogenome |
| title | Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) |
| title_full | Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) |
| title_fullStr | Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) |
| title_full_unstemmed | Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) |
| title_short | Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae) |
| title_sort | comprehensive molecular characterization of the mitochondrial genome of the takin lungworm i varestrongylus eleguneniensis i strongylida protostrongylidae |
| topic | <i>Varestrongylus</i> lungworm takins mitogenome |
| url | https://www.mdpi.com/1422-0067/23/21/13597 |
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