Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide
AIM: To investigate the protective effect of astaxanthin(AST)on human retinal pigment epithelial(RPE)cells against oxidative damage induced by hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>).<p>METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, r...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-07-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2015/7/201507007.pdf |
| Summary: | AIM: To investigate the protective effect of astaxanthin(AST)on human retinal pigment epithelial(RPE)cells against oxidative damage induced by hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>).<p>METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, rate of apoptosis was detected by flow cytometry and cell ultrastructure changes were observed under transmission electron microscope. <p>RESULTS:MTT results showed that cell activity elevated to(53.66%±3.25% and 70.43%±2.38% after 10<sup>-8</sup>mol/L and 10<sup>-4</sup>mol/L AST treated. The difference had statistically significant(<i>P</i><0.05)compared with oxidative injury group(38.76%±3.74%). Flow cytometry results showed that the apoptosis rate of RPE cells decreased to 30.23%±1.91% and 12.58%±2.12% in AST pretreated group, the difference was significant(<i>P</i><0.05)compared with oxidative injury group(42.50%±1.94%); Electron microscopy showed that the morphology of cells gradually improved accompanied with the concentration of AST elevated.<p>CONCLUSION:AST may inhibit hydrogen peroxide-induced apoptosis of RPE cells, it can provide reliable evidence for pursue effective medicine to prevent and treat retina injury. |
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| ISSN: | 1672-5123 1672-5123 |