Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide
AIM: To investigate the protective effect of astaxanthin(AST)on human retinal pigment epithelial(RPE)cells against oxidative damage induced by hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>).<p>METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, r...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-07-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2015/7/201507007.pdf |
| _version_ | 1818096602310508544 |
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| author | Hai-Rong Zhuang Ping Liu Xue-Zheng Hu |
| author_facet | Hai-Rong Zhuang Ping Liu Xue-Zheng Hu |
| author_sort | Hai-Rong Zhuang |
| collection | DOAJ |
| description | AIM: To investigate the protective effect of astaxanthin(AST)on human retinal pigment epithelial(RPE)cells against oxidative damage induced by hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>).<p>METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, rate of apoptosis was detected by flow cytometry and cell ultrastructure changes were observed under transmission electron microscope. <p>RESULTS:MTT results showed that cell activity elevated to(53.66%±3.25% and 70.43%±2.38% after 10<sup>-8</sup>mol/L and 10<sup>-4</sup>mol/L AST treated. The difference had statistically significant(<i>P</i><0.05)compared with oxidative injury group(38.76%±3.74%). Flow cytometry results showed that the apoptosis rate of RPE cells decreased to 30.23%±1.91% and 12.58%±2.12% in AST pretreated group, the difference was significant(<i>P</i><0.05)compared with oxidative injury group(42.50%±1.94%); Electron microscopy showed that the morphology of cells gradually improved accompanied with the concentration of AST elevated.<p>CONCLUSION:AST may inhibit hydrogen peroxide-induced apoptosis of RPE cells, it can provide reliable evidence for pursue effective medicine to prevent and treat retina injury. |
| first_indexed | 2024-12-10T23:07:14Z |
| format | Article |
| id | doaj.art-ed13ec71b5f54ef881ca18ca8662e2fa |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-10T23:07:14Z |
| publishDate | 2015-07-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-ed13ec71b5f54ef881ca18ca8662e2fa2022-12-22T01:30:02ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232015-07-011571148115010.3980/j.issn.1672-5123.2015.7.07Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxideHai-Rong Zhuang0Ping Liu1Xue-Zheng Hu2Department of Ophthalmology, Nongken General Hospital of Hainan, Haikou 570000, Hainan Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, ChinaDepartment of Ophthalmology, Nongken General Hospital of Hainan, Haikou 570000, Hainan Province, ChinaAIM: To investigate the protective effect of astaxanthin(AST)on human retinal pigment epithelial(RPE)cells against oxidative damage induced by hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>).<p>METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, rate of apoptosis was detected by flow cytometry and cell ultrastructure changes were observed under transmission electron microscope. <p>RESULTS:MTT results showed that cell activity elevated to(53.66%±3.25% and 70.43%±2.38% after 10<sup>-8</sup>mol/L and 10<sup>-4</sup>mol/L AST treated. The difference had statistically significant(<i>P</i><0.05)compared with oxidative injury group(38.76%±3.74%). Flow cytometry results showed that the apoptosis rate of RPE cells decreased to 30.23%±1.91% and 12.58%±2.12% in AST pretreated group, the difference was significant(<i>P</i><0.05)compared with oxidative injury group(42.50%±1.94%); Electron microscopy showed that the morphology of cells gradually improved accompanied with the concentration of AST elevated.<p>CONCLUSION:AST may inhibit hydrogen peroxide-induced apoptosis of RPE cells, it can provide reliable evidence for pursue effective medicine to prevent and treat retina injury.http://ies.ijo.cn/cn_publish/2015/7/201507007.pdfastaxanthinultravioletretinal pigment epithelial cellsapoptosis |
| spellingShingle | Hai-Rong Zhuang Ping Liu Xue-Zheng Hu Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide Guoji Yanke Zazhi astaxanthin ultraviolet retinal pigment epithelial cells apoptosis |
| title | Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| title_full | Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| title_fullStr | Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| title_full_unstemmed | Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| title_short | Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| title_sort | protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide |
| topic | astaxanthin ultraviolet retinal pigment epithelial cells apoptosis |
| url | http://ies.ijo.cn/cn_publish/2015/7/201507007.pdf |
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