<i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis
The study of <i>Aronia melanocarpa’s</i> (<i>A. melanocarpa</i>) biological activity is focused on obtaining the crude extract and separation of the flavonol compounds. The extraction and fractionation of <i>A. melanocarpa</i> fruits, followed by quantitative anal...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/16/2976 |
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| author | Kseniya Bushmeleva Alexandra Vyshtakalyuk Dmitriy Terenzhev Timur Belov Evgeniy Nikitin Vladimir Zobov |
| author_facet | Kseniya Bushmeleva Alexandra Vyshtakalyuk Dmitriy Terenzhev Timur Belov Evgeniy Nikitin Vladimir Zobov |
| author_sort | Kseniya Bushmeleva |
| collection | DOAJ |
| description | The study of <i>Aronia melanocarpa’s</i> (<i>A. melanocarpa</i>) biological activity is focused on obtaining the crude extract and separation of the flavonol compounds. The extraction and fractionation of <i>A. melanocarpa</i> fruits, followed by quantitative analysis, were accomplished using high-performance liquid chromatography and Darco G-60 filtering. This approach enabled the quantification of flavonoids within each fraction. The antioxidative, immunomodulating activities and cytotoxicity with respect to the lymphoblast cell line RPMI-1788 were studied. The flavonol extract of <i>A. melanocarpa</i> has been shown to have a high capacity to neutralize free DPPH and AAPH radicals in vitro. It also caused an accelerated ‘respiratory burst’ formation of neutrophils and an increase in the metabolic reserves of cells in rats exposed to cyclophosphamide. The reference solution (an equivalent quercetin-rutin blend) contributed to a decrease in lipid peroxidation, intensifying phagocytosis processes. The studied compounds demonstrated their low influence on the leukocyte blood profile in animals. |
| first_indexed | 2024-03-10T23:39:06Z |
| format | Article |
| id | doaj.art-ed148d127b3a443ca4075472b75a90a6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T23:39:06Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ed148d127b3a443ca4075472b75a90a62023-11-19T02:41:49ZengMDPI AGPlants2223-77472023-08-011216297610.3390/plants12162976<i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities AnalysisKseniya Bushmeleva0Alexandra Vyshtakalyuk1Dmitriy Terenzhev2Timur Belov3Evgeniy Nikitin4Vladimir Zobov5A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaA.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaA.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaA.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaA.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaA.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, Kazan 420088, RussiaThe study of <i>Aronia melanocarpa’s</i> (<i>A. melanocarpa</i>) biological activity is focused on obtaining the crude extract and separation of the flavonol compounds. The extraction and fractionation of <i>A. melanocarpa</i> fruits, followed by quantitative analysis, were accomplished using high-performance liquid chromatography and Darco G-60 filtering. This approach enabled the quantification of flavonoids within each fraction. The antioxidative, immunomodulating activities and cytotoxicity with respect to the lymphoblast cell line RPMI-1788 were studied. The flavonol extract of <i>A. melanocarpa</i> has been shown to have a high capacity to neutralize free DPPH and AAPH radicals in vitro. It also caused an accelerated ‘respiratory burst’ formation of neutrophils and an increase in the metabolic reserves of cells in rats exposed to cyclophosphamide. The reference solution (an equivalent quercetin-rutin blend) contributed to a decrease in lipid peroxidation, intensifying phagocytosis processes. The studied compounds demonstrated their low influence on the leukocyte blood profile in animals.https://www.mdpi.com/2223-7747/12/16/2976fruits of <i>Aronia melanocarpa</i>extractionflavonolsantioxidant capacityimmunomodulatory activityphagocytosis |
| spellingShingle | Kseniya Bushmeleva Alexandra Vyshtakalyuk Dmitriy Terenzhev Timur Belov Evgeniy Nikitin Vladimir Zobov <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis Plants fruits of <i>Aronia melanocarpa</i> extraction flavonols antioxidant capacity immunomodulatory activity phagocytosis |
| title | <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis |
| title_full | <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis |
| title_fullStr | <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis |
| title_full_unstemmed | <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis |
| title_short | <i>Aronia melanocarpa</i> Flavonol Extract—Antiradical and Immunomodulating Activities Analysis |
| title_sort | i aronia melanocarpa i flavonol extract antiradical and immunomodulating activities analysis |
| topic | fruits of <i>Aronia melanocarpa</i> extraction flavonols antioxidant capacity immunomodulatory activity phagocytosis |
| url | https://www.mdpi.com/2223-7747/12/16/2976 |
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