Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds
This study aimed to evaluate the productive performance and microbiota variation in the jejunum and cecum of two rabbit breeds with different growth rates. This study was carried out on Native Middle-Egypt Breed (NMER) and Giant Flanders (GF) rabbits from 5 weeks to 12 weeks of age. Twenty NMER (NM)...
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/14/2294 |
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| author | El-Sayed M. Abdel-Kafy Kamel I. Kamel Marco Severgnini Shama H. A. Morsy Paola Cremonesi Shereen S. Ghoneim Gabriele Brecchia Neama I. Ali Yasmein Z. Abdel-Ghafar Wael A. H. Ali Hoda M. A. Shabaan |
| author_facet | El-Sayed M. Abdel-Kafy Kamel I. Kamel Marco Severgnini Shama H. A. Morsy Paola Cremonesi Shereen S. Ghoneim Gabriele Brecchia Neama I. Ali Yasmein Z. Abdel-Ghafar Wael A. H. Ali Hoda M. A. Shabaan |
| author_sort | El-Sayed M. Abdel-Kafy |
| collection | DOAJ |
| description | This study aimed to evaluate the productive performance and microbiota variation in the jejunum and cecum of two rabbit breeds with different growth rates. This study was carried out on Native Middle-Egypt Breed (NMER) and Giant Flanders (GF) rabbits from 5 weeks to 12 weeks of age. Twenty NMER (NM) and GF male rabbits were slaughtered, and the jejunum and cecum tracts were collected to assay gut microbiota composition via 16S ribosomal RNA (rRNA) gene sequencing and histology examination. At 12 weeks of age, daily weight gain, villus height in the jejunum, total protein, and albumin were higher in GF rabbits than in NMER rabbits. Also, the jejunal villi of GF were well arranged in their dense borders. The microbiota between the jejunum and cecum was significantly different in terms of Beta-diversity. A significant correlation between <i>Enterococcus</i> (jejunum NM samples) and <i>Lactobacillus</i> (cecum GF samples) with body weight and weight gain was found (<i>p</i> < 0.05). Moreover, Escherichia-Shigella in the cecum of NM was significantly correlated with weight gain (<i>p</i> < 0.05). The most abundant genera identified in the jejunal and cecal contents of GF were generally beneficial microbiota. They may also play a role in reducing the pathogenic effects of <i>Escherichia coli</i> in these rabbits. |
| first_indexed | 2024-03-11T01:23:03Z |
| format | Article |
| id | doaj.art-ed1526a6f9dd43c6ad2b74b35a9e86e0 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:23:03Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ed1526a6f9dd43c6ad2b74b35a9e86e02023-11-18T18:00:18ZengMDPI AGAnimals2076-26152023-07-011314229410.3390/ani13142294Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit BreedsEl-Sayed M. Abdel-Kafy0Kamel I. Kamel1Marco Severgnini2Shama H. A. Morsy3Paola Cremonesi4Shereen S. Ghoneim5Gabriele Brecchia6Neama I. Ali7Yasmein Z. Abdel-Ghafar8Wael A. H. Ali9Hoda M. A. Shabaan10Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptInstitute of Biomedical Technologies, National Research Council (ITB-CNR), Via f.lli Cervi, 93, 20054 Segrate, ItalyAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptInstitute of Agricultural Biology and Biotechnology, National Research Council (IBBA-CNR), Via Einstein s/n, 26900 Lodi, ItalyAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptDepartment of Veterinary Medicine, University of Milano, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Cell Biology, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, EgyptAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptAnimal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Giza 12651, EgyptThis study aimed to evaluate the productive performance and microbiota variation in the jejunum and cecum of two rabbit breeds with different growth rates. This study was carried out on Native Middle-Egypt Breed (NMER) and Giant Flanders (GF) rabbits from 5 weeks to 12 weeks of age. Twenty NMER (NM) and GF male rabbits were slaughtered, and the jejunum and cecum tracts were collected to assay gut microbiota composition via 16S ribosomal RNA (rRNA) gene sequencing and histology examination. At 12 weeks of age, daily weight gain, villus height in the jejunum, total protein, and albumin were higher in GF rabbits than in NMER rabbits. Also, the jejunal villi of GF were well arranged in their dense borders. The microbiota between the jejunum and cecum was significantly different in terms of Beta-diversity. A significant correlation between <i>Enterococcus</i> (jejunum NM samples) and <i>Lactobacillus</i> (cecum GF samples) with body weight and weight gain was found (<i>p</i> < 0.05). Moreover, Escherichia-Shigella in the cecum of NM was significantly correlated with weight gain (<i>p</i> < 0.05). The most abundant genera identified in the jejunal and cecal contents of GF were generally beneficial microbiota. They may also play a role in reducing the pathogenic effects of <i>Escherichia coli</i> in these rabbits.https://www.mdpi.com/2076-2615/13/14/2294rabbitsmicrobiota16S rRNA gene sequencingjejunumcecumbreeds |
| spellingShingle | El-Sayed M. Abdel-Kafy Kamel I. Kamel Marco Severgnini Shama H. A. Morsy Paola Cremonesi Shereen S. Ghoneim Gabriele Brecchia Neama I. Ali Yasmein Z. Abdel-Ghafar Wael A. H. Ali Hoda M. A. Shabaan Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds Animals rabbits microbiota 16S rRNA gene sequencing jejunum cecum breeds |
| title | Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds |
| title_full | Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds |
| title_fullStr | Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds |
| title_full_unstemmed | Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds |
| title_short | Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds |
| title_sort | diversity and co occurrence pattern analysis of cecal and jejunal microbiota in two rabbit breeds |
| topic | rabbits microbiota 16S rRNA gene sequencing jejunum cecum breeds |
| url | https://www.mdpi.com/2076-2615/13/14/2294 |
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