Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring a...
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1540 |
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| author | Hegger Fritsch Felicidade Mota Pereira Erica Azevedo Costa Vagner Fonseca Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguiar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de Sá George Roma Santos Willadesmon Santos Silva Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Luiz Carlos Junior Alcantara |
| author_facet | Hegger Fritsch Felicidade Mota Pereira Erica Azevedo Costa Vagner Fonseca Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguiar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de Sá George Roma Santos Willadesmon Santos Silva Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Luiz Carlos Junior Alcantara |
| author_sort | Hegger Fritsch |
| collection | DOAJ |
| description | During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Ceará and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country. |
| first_indexed | 2024-03-09T12:51:28Z |
| format | Article |
| id | doaj.art-ed159742e8a44d419389810035a4c546 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:51:28Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ed159742e8a44d419389810035a4c5462023-11-30T22:05:46ZengMDPI AGViruses1999-49152022-07-01147154010.3390/v14071540Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in BrazilHegger Fritsch0Felicidade Mota Pereira1Erica Azevedo Costa2Vagner Fonseca3Stephane Tosta4Joilson Xavier5Flavia Levy6Carla de Oliveira7Gabriela Menezes8Jaqueline Lima9Lenisa Santos10Luciana Silva11Vanessa Nardy12Marcela Kelly Gómez Astete13Beatriz Senra Álvares da Silva Santos14Nágila Rocha Aguiar15Maria Isabel Maldonado Coelho Guedes16Guilherme Canhestro de Faria17Ronaldo Furtini18Safira Rachel Milanez Drumond19Gabriel Muricy Cunha20Marcia São Pedro Leal Souza21Ronaldo de Jesus22Sara A. Franco Guimarães23Italo Coelho Nuno24Ian Carlos Brito de Santana25José Eduardo Ungar de Sá26George Roma Santos27Willadesmon Santos Silva28Thiago Ferreira Guedes29Emerson Luiz Lima Araújo30Rodrigo Fabiano do Carmo Said31Carlos Frederico Campelo de Albuquerque32Cassio Roberto Leonel Peterka33Alessandro Pecego Martins Romano34Rivaldo Venâncio da Cunha35Ana Maria Bispo de Filippis36Arabela Leal e Silva de Mello37Marta Giovanetti38Luiz Carlos Junior Alcantara39Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilOrganização Pan-Americana de Saúde/Organização Mundial de Saúde, Brasilia 37650-000, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, BrazilLaboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilLaboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, BrazilLaboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, BrazilLaboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, BrazilSecretary of Health of the State of Bahia (SESAB), Salvador 40301-110, BrazilSecretary of Health of the State of Bahia (SESAB), Salvador 40301-110, BrazilCoordenação Geral dos Laboratórios de Saúde Pública, Secretaria de Vigilância em Saúde-Brazil, Brasília 70719-040, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilCoordenação Geral dos Laboratórios de Saúde Pública, Secretaria de Vigilância em Saúde-Brazil, Brasília 70719-040, BrazilCoordenação Geral dos Laboratórios de Saúde Pública, Secretaria de Vigilância em Saúde-Brazil, Brasília 70719-040, BrazilOrganização Pan-Americana de Saúde/Organização Mundial de Saúde, Brasilia 37650-000, BrazilOrganização Pan-Americana de Saúde/Organização Mundial de Saúde, Brasilia 37650-000, BrazilCoordenação Geral das Arboviroses, Secretaria de Vigilância em Saúde (CGARB/SVS-MS), Brasilia 37650-000, BrazilCoordenação Geral das Arboviroses, Secretaria de Vigilância em Saúde (CGARB/SVS-MS), Brasilia 37650-000, BrazilFundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro 21040-360, BrazilLaboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, BrazilLaboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, BrazilLaboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, BrazilLaboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, BrazilDuring these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Ceará and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country.https://www.mdpi.com/1999-4915/14/7/1540WNVnanopore sequencinggenomic monitoringBrazil |
| spellingShingle | Hegger Fritsch Felicidade Mota Pereira Erica Azevedo Costa Vagner Fonseca Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguiar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de Sá George Roma Santos Willadesmon Santos Silva Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Luiz Carlos Junior Alcantara Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil Viruses WNV nanopore sequencing genomic monitoring Brazil |
| title | Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil |
| title_full | Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil |
| title_fullStr | Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil |
| title_full_unstemmed | Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil |
| title_short | Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil |
| title_sort | retrospective investigation in horses with encephalitis reveals unnoticed circulation of west nile virus in brazil |
| topic | WNV nanopore sequencing genomic monitoring Brazil |
| url | https://www.mdpi.com/1999-4915/14/7/1540 |
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