Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone
Abstract Background In the bone tissue engineering domain, seed cells, scaffold and cell-scaffold composites are three focuses. In this study, the feasibility of using allogeneic adipose-derived stem cells(ADSCs) combined with heterogeneous deproteinized bone (HDB) to repair segmental radial defects...
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0817-1 |
| _version_ | 1818839237891457024 |
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| author | Jia Liu Peng Zhou Yu Long Chunxia Huang Danna Chen |
| author_facet | Jia Liu Peng Zhou Yu Long Chunxia Huang Danna Chen |
| author_sort | Jia Liu |
| collection | DOAJ |
| description | Abstract Background In the bone tissue engineering domain, seed cells, scaffold and cell-scaffold composites are three focuses. In this study, the feasibility of using allogeneic adipose-derived stem cells(ADSCs) combined with heterogeneous deproteinized bone (HDB) to repair segmental radial defects was investigated by observing the repair of the defect area. Methods ADSCs were cultured in vitro, purified, antigen-detected and osteogenic differentiation potency-measured; then, the ADSCs of the third generation were seeded into HDB to prepare an ADSCs-HDB composite partly with osteogenesis induced cells. Sixty Wistar rats were randomly divided into four groups with 15 in each group. A bone defect (4 mm in length) was created at the left radius in each rat. Two kinds of ADSCs-HDB composites were implanted in the ADSCs osteogenesis group or ADSCs group; HDB was implanted in the negative control group; nothing was filled in the blank control group. The bone defect repair was evaluated by gross observation, molybdenum target X-ray examination and histological analyses after surgery. Results Gross observation: the bone defect area was completely filled and difficult to recognize in the ADSCs osteogenesis group. The connection of the ADSCs group was strong, but the implants were clearly identifiable. The joints of the negative control group were slightly thick but the connection was unstable. In the blank control group, kermesinus tissue was between the two ends and bones were not connected after 8 weeks. Molybdenum target X-ray examinations: In the ADSCs osteogenesis group, evident bridges in the graft were observed in the defects in the fourth week; the defects were filled with new bone completely and a marrow cavity appeared at 8 weeks. In the ADSCs group, there were some callus formations, but the radial defect was still obvious at 8 weeks. In the negative control group, fracture lines were clear. In the blank control group, no osseous bridges were observed, which resulted in bone nonunion eventually in 8 weeks. There were significant differences in the callus density between experimental groups and the blank control group at 4 and 8 weeks (P < 0.01). Histological measures showed that the rate and quality of the new bone formation and remodelling was significantly different between the experimental and control groups. Conclusions A composite of ADSCs-HDB has a strong osteogenic ability. It can repair segmental bone defects well and is promising to serve as grafting material in bone tissue engineering. |
| first_indexed | 2024-12-19T03:51:06Z |
| format | Article |
| id | doaj.art-ed17453e06744650b25190f71d3afc00 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-19T03:51:06Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
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| series | Stem Cell Research & Therapy |
| spelling | doaj.art-ed17453e06744650b25190f71d3afc002022-12-21T20:36:59ZengBMCStem Cell Research & Therapy1757-65122018-03-019111010.1186/s13287-018-0817-1Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized boneJia Liu0Peng Zhou1Yu Long2Chunxia Huang3Danna Chen4Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Changsha Medical UniversityDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Changsha Medical UniversityDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Changsha Medical UniversityDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Changsha Medical UniversityDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Changsha Medical UniversityAbstract Background In the bone tissue engineering domain, seed cells, scaffold and cell-scaffold composites are three focuses. In this study, the feasibility of using allogeneic adipose-derived stem cells(ADSCs) combined with heterogeneous deproteinized bone (HDB) to repair segmental radial defects was investigated by observing the repair of the defect area. Methods ADSCs were cultured in vitro, purified, antigen-detected and osteogenic differentiation potency-measured; then, the ADSCs of the third generation were seeded into HDB to prepare an ADSCs-HDB composite partly with osteogenesis induced cells. Sixty Wistar rats were randomly divided into four groups with 15 in each group. A bone defect (4 mm in length) was created at the left radius in each rat. Two kinds of ADSCs-HDB composites were implanted in the ADSCs osteogenesis group or ADSCs group; HDB was implanted in the negative control group; nothing was filled in the blank control group. The bone defect repair was evaluated by gross observation, molybdenum target X-ray examination and histological analyses after surgery. Results Gross observation: the bone defect area was completely filled and difficult to recognize in the ADSCs osteogenesis group. The connection of the ADSCs group was strong, but the implants were clearly identifiable. The joints of the negative control group were slightly thick but the connection was unstable. In the blank control group, kermesinus tissue was between the two ends and bones were not connected after 8 weeks. Molybdenum target X-ray examinations: In the ADSCs osteogenesis group, evident bridges in the graft were observed in the defects in the fourth week; the defects were filled with new bone completely and a marrow cavity appeared at 8 weeks. In the ADSCs group, there were some callus formations, but the radial defect was still obvious at 8 weeks. In the negative control group, fracture lines were clear. In the blank control group, no osseous bridges were observed, which resulted in bone nonunion eventually in 8 weeks. There were significant differences in the callus density between experimental groups and the blank control group at 4 and 8 weeks (P < 0.01). Histological measures showed that the rate and quality of the new bone formation and remodelling was significantly different between the experimental and control groups. Conclusions A composite of ADSCs-HDB has a strong osteogenic ability. It can repair segmental bone defects well and is promising to serve as grafting material in bone tissue engineering.http://link.springer.com/article/10.1186/s13287-018-0817-1Adipose-derived stem cellsDifferentiationOsteogenesisDeproteinized boneBone defectMolybdenum target X-ray |
| spellingShingle | Jia Liu Peng Zhou Yu Long Chunxia Huang Danna Chen Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone Stem Cell Research & Therapy Adipose-derived stem cells Differentiation Osteogenesis Deproteinized bone Bone defect Molybdenum target X-ray |
| title | Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone |
| title_full | Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone |
| title_fullStr | Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone |
| title_full_unstemmed | Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone |
| title_short | Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone |
| title_sort | repair of bone defects in rat radii with a composite of allogeneic adipose derived stem cells and heterogeneous deproteinized bone |
| topic | Adipose-derived stem cells Differentiation Osteogenesis Deproteinized bone Bone defect Molybdenum target X-ray |
| url | http://link.springer.com/article/10.1186/s13287-018-0817-1 |
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