Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties
Background/purpose: Resin-modified glass ionomers (RMGIs) have been recommended as liner and cement to provide the teeth with mechanical support, a chemical barrier, and thermal insulation. Acemannan, the main polysaccharide extracted from Aloe vera, is a promising inductive material in vitro and in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790223001848 |
| _version_ | 1827292817228038144 |
|---|---|
| author | Thant Aye Aye Sangvanich Polkit Inchudech Klaijan Kuvieng Nachanok Lalitkanjanakul Salil Thunyakitpisal Pasutha |
| author_facet | Thant Aye Aye Sangvanich Polkit Inchudech Klaijan Kuvieng Nachanok Lalitkanjanakul Salil Thunyakitpisal Pasutha |
| author_sort | Thant Aye Aye |
| collection | DOAJ |
| description | Background/purpose: Resin-modified glass ionomers (RMGIs) have been recommended as liner and cement to provide the teeth with mechanical support, a chemical barrier, and thermal insulation. Acemannan, the main polysaccharide extracted from Aloe vera, is a promising inductive material in vitro and in vivo. This study aimed to develop acemannan-containing bioactive resin-modified glass ionomers (RMGIs). Materials and methods: Acemannan (3%, 5%, and 10%) was added to the three types of RMGIs (RU-HBM1/Fuji II LC/Vitrebond) to generate 3%, 5%, and 10% aceRMGIs (aceRU/aceFuji/aceVB). The materials were evaluated for depth of cure/flexural strength/cumulative fluoride ion release. Cell viability and vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) secretion were determined using MTT/apoptosis/necrosis assays, and ELISA kits, respectively. RMGI without acemannan were used as controls. Results: The aceRMGIs met the ISO requirements for depth of cure and flexural strength. Adding 10% acemannan increased the cumulative fluoride release in the RU and FJ groups, but slightly decreased it in the VB group (P < 0.05). The MTT assay revealed 10% aceRU and all aceFJ groups significantly increased cell viability compared with each control group (P < 0.05). Apoptosis/necrosis assay showed the biocompatibility of all aceRMGIs. Adding acemannan to RMGIs significantly induced VEGF expression in a dose dependent manner while 5% and 10% aceRU significantly induced BMP-2 expression compared with RU group (P < 0.05). Conclusion: We conclude that 5–10% acemannan in RMGI is the optimal concentration based on its physical properties and ability to induce pulp cell proliferation and growth factor secretion. |
| first_indexed | 2024-04-24T13:12:16Z |
| format | Article |
| id | doaj.art-ed186071b04b4e7e98a4a3f2b43aedd9 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-24T13:12:16Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-ed186071b04b4e7e98a4a3f2b43aedd92024-04-05T04:40:12ZengElsevierJournal of Dental Sciences1991-79022024-04-0119210611069Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological propertiesThant Aye Aye0Sangvanich Polkit1Inchudech Klaijan2Kuvieng Nachanok3Lalitkanjanakul Salil4Thunyakitpisal Pasutha5Dental Biomaterials Science Program, Graduate School, Chulalongkorn University, Bangkok, Thailand; Research Unit of Herbal Medicine, Biomaterial, and Material for Dental Treatment, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, ThailandResearch Unit of Herbal Medicine, Biomaterial, and Material for Dental Treatment, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandResearch Unit of Herbal Medicine, Biomaterial, and Material for Dental Treatment, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandResearch Unit of Herbal Medicine, Biomaterial, and Material for Dental Treatment, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandResearch Unit of Herbal Medicine, Biomaterial, and Material for Dental Treatment, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; Institute of Dentistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand; Corresponding author. Institute of Dentistry, Suranaree University of Technology, 111 University avenue, Muang, Nakhon Ratchasima 30000, Thailand.Background/purpose: Resin-modified glass ionomers (RMGIs) have been recommended as liner and cement to provide the teeth with mechanical support, a chemical barrier, and thermal insulation. Acemannan, the main polysaccharide extracted from Aloe vera, is a promising inductive material in vitro and in vivo. This study aimed to develop acemannan-containing bioactive resin-modified glass ionomers (RMGIs). Materials and methods: Acemannan (3%, 5%, and 10%) was added to the three types of RMGIs (RU-HBM1/Fuji II LC/Vitrebond) to generate 3%, 5%, and 10% aceRMGIs (aceRU/aceFuji/aceVB). The materials were evaluated for depth of cure/flexural strength/cumulative fluoride ion release. Cell viability and vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) secretion were determined using MTT/apoptosis/necrosis assays, and ELISA kits, respectively. RMGI without acemannan were used as controls. Results: The aceRMGIs met the ISO requirements for depth of cure and flexural strength. Adding 10% acemannan increased the cumulative fluoride release in the RU and FJ groups, but slightly decreased it in the VB group (P < 0.05). The MTT assay revealed 10% aceRU and all aceFJ groups significantly increased cell viability compared with each control group (P < 0.05). Apoptosis/necrosis assay showed the biocompatibility of all aceRMGIs. Adding acemannan to RMGIs significantly induced VEGF expression in a dose dependent manner while 5% and 10% aceRU significantly induced BMP-2 expression compared with RU group (P < 0.05). Conclusion: We conclude that 5–10% acemannan in RMGI is the optimal concentration based on its physical properties and ability to induce pulp cell proliferation and growth factor secretion.http://www.sciencedirect.com/science/article/pii/S1991790223001848Aloe veraBiomaterialCytotoxicityDepth of cureFlexural strengthRegeneration |
| spellingShingle | Thant Aye Aye Sangvanich Polkit Inchudech Klaijan Kuvieng Nachanok Lalitkanjanakul Salil Thunyakitpisal Pasutha Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties Journal of Dental Sciences Aloe vera Biomaterial Cytotoxicity Depth of cure Flexural strength Regeneration |
| title | Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| title_full | Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| title_fullStr | Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| title_full_unstemmed | Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| title_short | Acemannan-containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| title_sort | acemannan containing bioactive resin modified glass ionomer demonstrates satisfactory physical and biological properties |
| topic | Aloe vera Biomaterial Cytotoxicity Depth of cure Flexural strength Regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S1991790223001848 |
| work_keys_str_mv | AT thantayeaye acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties AT sangvanichpolkit acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties AT inchudechklaijan acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties AT kuviengnachanok acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties AT lalitkanjanakulsalil acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties AT thunyakitpisalpasutha acemannancontainingbioactiveresinmodifiedglassionomerdemonstratessatisfactoryphysicalandbiologicalproperties |