Effect of hepatitis C infection on HIV-induced apoptosis.
BACKGROUND: Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruse...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3788068?pdf=render |
| _version_ | 1818510202587054080 |
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| author | Tomasz Laskus Karen V Kibler Marcin Chmielewski Jeffrey Wilkinson Debra Adair Andrzej Horban Grzegorz Stańczak Marek Radkowski |
| author_facet | Tomasz Laskus Karen V Kibler Marcin Chmielewski Jeffrey Wilkinson Debra Adair Andrzej Horban Grzegorz Stańczak Marek Radkowski |
| author_sort | Tomasz Laskus |
| collection | DOAJ |
| description | BACKGROUND: Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruses. METHODS: We studied CD4+ and CD8+ T cell and CD19+ B cell apoptosis in 104 HIV-positive patients (56 were also HCV-positive) and in 22 HCV/HIV-coinfected patients treated for chronic hepatitis C with pegylated interferon and ribavirin. We also analyzed HCV/HIV coinfection in a Daudi B-cell line expressing CD4 and susceptible to both HCV and HIV infection. Apoptosis was measured by AnnexinV staining. RESULTS: HCV/HIV coinfected patients had lower CD4+ and CD8+ T cell apoptosis and higher CD19+ B cell apoptosis than those with HIV monoinfection. Furthermore, anti-HCV treatment of HCV/HIV coinfected patients was followed by an increase of CD4+ and CD8+ T cell apoptosis and a decrease of CD19+ B cell apoptosis. In the Daudi CD4+ cell line, presence of HCV infection facilitated HIV replication, however, decreased the rate of HIV-related cell death. CONCLUSION: In HCV/HIV coinfected patients T-cells were found to be destroyed at a slower rate than in HIV monoinfected patients. These results suggest that HCV is a molecular-level determinant in HIV disease. |
| first_indexed | 2024-12-10T22:56:05Z |
| format | Article |
| id | doaj.art-ed19e2e96d6447208f163d1e4f1c967e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:56:05Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ed19e2e96d6447208f163d1e4f1c967e2022-12-22T01:30:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7592110.1371/journal.pone.0075921Effect of hepatitis C infection on HIV-induced apoptosis.Tomasz LaskusKaren V KiblerMarcin ChmielewskiJeffrey WilkinsonDebra AdairAndrzej HorbanGrzegorz StańczakMarek RadkowskiBACKGROUND: Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruses. METHODS: We studied CD4+ and CD8+ T cell and CD19+ B cell apoptosis in 104 HIV-positive patients (56 were also HCV-positive) and in 22 HCV/HIV-coinfected patients treated for chronic hepatitis C with pegylated interferon and ribavirin. We also analyzed HCV/HIV coinfection in a Daudi B-cell line expressing CD4 and susceptible to both HCV and HIV infection. Apoptosis was measured by AnnexinV staining. RESULTS: HCV/HIV coinfected patients had lower CD4+ and CD8+ T cell apoptosis and higher CD19+ B cell apoptosis than those with HIV monoinfection. Furthermore, anti-HCV treatment of HCV/HIV coinfected patients was followed by an increase of CD4+ and CD8+ T cell apoptosis and a decrease of CD19+ B cell apoptosis. In the Daudi CD4+ cell line, presence of HCV infection facilitated HIV replication, however, decreased the rate of HIV-related cell death. CONCLUSION: In HCV/HIV coinfected patients T-cells were found to be destroyed at a slower rate than in HIV monoinfected patients. These results suggest that HCV is a molecular-level determinant in HIV disease.http://europepmc.org/articles/PMC3788068?pdf=render |
| spellingShingle | Tomasz Laskus Karen V Kibler Marcin Chmielewski Jeffrey Wilkinson Debra Adair Andrzej Horban Grzegorz Stańczak Marek Radkowski Effect of hepatitis C infection on HIV-induced apoptosis. PLoS ONE |
| title | Effect of hepatitis C infection on HIV-induced apoptosis. |
| title_full | Effect of hepatitis C infection on HIV-induced apoptosis. |
| title_fullStr | Effect of hepatitis C infection on HIV-induced apoptosis. |
| title_full_unstemmed | Effect of hepatitis C infection on HIV-induced apoptosis. |
| title_short | Effect of hepatitis C infection on HIV-induced apoptosis. |
| title_sort | effect of hepatitis c infection on hiv induced apoptosis |
| url | http://europepmc.org/articles/PMC3788068?pdf=render |
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