Protective effect of thymol on glycerol-induced acute kidney injury
AbstractAcute kidney injury (AKI) is a syndrome characterized by an accelerating decrease in renal function in a short time. Thymol is one of the main components of thyme species and has a variety of pharmacological effects. Here, we investigated whether thymol could ameliorate rhabdomyolysis (RM)-i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Renal Failure |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2227728 |
| _version_ | 1797658543604629504 |
|---|---|
| author | Qinglian Wang Guanghui Qi Hongwei Zhou Fajuan Cheng Xiaowei Yang Xiang Liu Rong Wang |
| author_facet | Qinglian Wang Guanghui Qi Hongwei Zhou Fajuan Cheng Xiaowei Yang Xiang Liu Rong Wang |
| author_sort | Qinglian Wang |
| collection | DOAJ |
| description | AbstractAcute kidney injury (AKI) is a syndrome characterized by an accelerating decrease in renal function in a short time. Thymol is one of the main components of thyme species and has a variety of pharmacological effects. Here, we investigated whether thymol could ameliorate rhabdomyolysis (RM)-induced AKI and its related mechanism. Glycerol was used to induce RM-associated AKI in rats. Rats received thymol (20 mg/kg/day or 40 mg/kg/day) gavage 24 h before glycerol injection until 72 h after injection daily. Kidney injury was identified by measuring serum creatinine (Scr) and urea levels and by H&E and PAS staining and immunohistochemistry (the expression of proliferating cell nuclear antigen (PCNA)). Renal superoxide dismutase (SOD), malondialdehyde (MDA), and oxidative stress-related Nrf2/HO-1 signaling pathways were measured. The expression of the inflammatory markers TNF-α, IL-6, MCP-1, and NF-κB was assessed by ELISA and western blotting. Finally, the expression of the PI3K/Akt signaling pathway was detected by western blotting. Glycerol administration induced obvious renal histologic damage and increased Scr, urea, and PCNA expression. Notably, thymol treatment attenuated these structural and functional changes and prevented renal oxidative stress, inflammatory damage and PI3K/Akt pathway downregulation associated with glycerol-induced AKI. In conclusion, thymol might have potential applications in the amelioration of AKI via its antioxidant and anti-inflammatory effects and upregulation of the PI3K/Akt signaling pathway. |
| first_indexed | 2024-03-11T18:00:35Z |
| format | Article |
| id | doaj.art-ed1a7fbdeab249049614dd268e3ae41c |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-03-11T18:00:35Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-ed1a7fbdeab249049614dd268e3ae41c2023-10-17T09:23:23ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492023-12-0145110.1080/0886022X.2023.2227728Protective effect of thymol on glycerol-induced acute kidney injuryQinglian Wang0Guanghui Qi1Hongwei Zhou2Fajuan Cheng3Xiaowei Yang4Xiang Liu5Rong Wang6Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaDepartment of Urology, Zibo First Hospital, Zibo, ChinaDepartment of Nephrology, Pingyi County People’s Hospital, Linyi, ChinaDepartment of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaDepartment of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaDepartment of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaDepartment of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaAbstractAcute kidney injury (AKI) is a syndrome characterized by an accelerating decrease in renal function in a short time. Thymol is one of the main components of thyme species and has a variety of pharmacological effects. Here, we investigated whether thymol could ameliorate rhabdomyolysis (RM)-induced AKI and its related mechanism. Glycerol was used to induce RM-associated AKI in rats. Rats received thymol (20 mg/kg/day or 40 mg/kg/day) gavage 24 h before glycerol injection until 72 h after injection daily. Kidney injury was identified by measuring serum creatinine (Scr) and urea levels and by H&E and PAS staining and immunohistochemistry (the expression of proliferating cell nuclear antigen (PCNA)). Renal superoxide dismutase (SOD), malondialdehyde (MDA), and oxidative stress-related Nrf2/HO-1 signaling pathways were measured. The expression of the inflammatory markers TNF-α, IL-6, MCP-1, and NF-κB was assessed by ELISA and western blotting. Finally, the expression of the PI3K/Akt signaling pathway was detected by western blotting. Glycerol administration induced obvious renal histologic damage and increased Scr, urea, and PCNA expression. Notably, thymol treatment attenuated these structural and functional changes and prevented renal oxidative stress, inflammatory damage and PI3K/Akt pathway downregulation associated with glycerol-induced AKI. In conclusion, thymol might have potential applications in the amelioration of AKI via its antioxidant and anti-inflammatory effects and upregulation of the PI3K/Akt signaling pathway.https://www.tandfonline.com/doi/10.1080/0886022X.2023.2227728RhabdomyolysisAKIoxidative stressinflammationPI3K/Akt |
| spellingShingle | Qinglian Wang Guanghui Qi Hongwei Zhou Fajuan Cheng Xiaowei Yang Xiang Liu Rong Wang Protective effect of thymol on glycerol-induced acute kidney injury Renal Failure Rhabdomyolysis AKI oxidative stress inflammation PI3K/Akt |
| title | Protective effect of thymol on glycerol-induced acute kidney injury |
| title_full | Protective effect of thymol on glycerol-induced acute kidney injury |
| title_fullStr | Protective effect of thymol on glycerol-induced acute kidney injury |
| title_full_unstemmed | Protective effect of thymol on glycerol-induced acute kidney injury |
| title_short | Protective effect of thymol on glycerol-induced acute kidney injury |
| title_sort | protective effect of thymol on glycerol induced acute kidney injury |
| topic | Rhabdomyolysis AKI oxidative stress inflammation PI3K/Akt |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2227728 |
| work_keys_str_mv | AT qinglianwang protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT guanghuiqi protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT hongweizhou protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT fajuancheng protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT xiaoweiyang protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT xiangliu protectiveeffectofthymolonglycerolinducedacutekidneyinjury AT rongwang protectiveeffectofthymolonglycerolinducedacutekidneyinjury |