Regulation of trophoblast beta1-integrin expression by contact with endothelial cells
<p>Abstract</p> <p>Background</p> <p>In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade uterine matrix, and enter the uterine vasculature. Invasive trophoblasts show increased expression of β1 integrin. Since trophoblast mig...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2004-06-01
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| Series: | Cell Communication and Signaling |
| Online Access: | http://www.biosignaling.com/content/2/1/4 |
| _version_ | 1811246204961423360 |
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| author | Thirkill Twanda L Hendren Sonia R Soghomonians Arlen Mariano Natalie F Barakat Abdul I Douglas Gordon C |
| author_facet | Thirkill Twanda L Hendren Sonia R Soghomonians Arlen Mariano Natalie F Barakat Abdul I Douglas Gordon C |
| author_sort | Thirkill Twanda L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade uterine matrix, and enter the uterine vasculature. Invasive trophoblasts show increased expression of β1 integrin. Since trophoblast migration within the uterine vasculature involves trophoblast attachment to endothelial cells lining the vessel walls, this raises the possibility that cell-cell contact and/or factors released by endothelial cells could regulate trophoblast integrin expression. To test this, we used an in vitro system consisting of early gestation macaque trophoblasts co-cultured on top of uterine microvascular endothelial cells.</p> <p>Results</p> <p>When cultured alone, trophoblasts expressed low levels of β1 integrin as determined by quantitative immunofluorescence microscopy. When trophoblasts were cultured on top of endothelial cells for 24 h, the expression of trophoblast β1 integrin was significantly increased as determined by image analysis. β1 Integrin expression was not increased when trophoblasts were cultured with endothelial cell-conditioned medium, suggesting that upregulation requires direct contact between trophoblasts and endothelial cells. To identify endothelial cell surface molecules responsible for induction of trophoblast integrin expression, trophoblasts were cultured in dishes coated with recombinant platelet endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), or αVβ3 integrin. Trophoblast β1 integrin expression (assessed by immunofluorescence microscopy and Western blotting) was increased when PECAM-1 or αVβ3 integrin, but not ICAM-1, was used as substrate.</p> <p>Conclusions</p> <p>Direct contact between trophoblasts and endothelial cells increases the expression of trophoblast β1 integrin.</p> |
| first_indexed | 2024-04-12T14:50:17Z |
| format | Article |
| id | doaj.art-ed1ac978462041cb8e6c555360e55e70 |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-04-12T14:50:17Z |
| publishDate | 2004-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-ed1ac978462041cb8e6c555360e55e702022-12-22T03:28:31ZengBMCCell Communication and Signaling1478-811X2004-06-0121410.1186/1478-811X-2-4Regulation of trophoblast beta1-integrin expression by contact with endothelial cellsThirkill Twanda LHendren Sonia RSoghomonians ArlenMariano Natalie FBarakat Abdul IDouglas Gordon C<p>Abstract</p> <p>Background</p> <p>In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade uterine matrix, and enter the uterine vasculature. Invasive trophoblasts show increased expression of β1 integrin. Since trophoblast migration within the uterine vasculature involves trophoblast attachment to endothelial cells lining the vessel walls, this raises the possibility that cell-cell contact and/or factors released by endothelial cells could regulate trophoblast integrin expression. To test this, we used an in vitro system consisting of early gestation macaque trophoblasts co-cultured on top of uterine microvascular endothelial cells.</p> <p>Results</p> <p>When cultured alone, trophoblasts expressed low levels of β1 integrin as determined by quantitative immunofluorescence microscopy. When trophoblasts were cultured on top of endothelial cells for 24 h, the expression of trophoblast β1 integrin was significantly increased as determined by image analysis. β1 Integrin expression was not increased when trophoblasts were cultured with endothelial cell-conditioned medium, suggesting that upregulation requires direct contact between trophoblasts and endothelial cells. To identify endothelial cell surface molecules responsible for induction of trophoblast integrin expression, trophoblasts were cultured in dishes coated with recombinant platelet endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), or αVβ3 integrin. Trophoblast β1 integrin expression (assessed by immunofluorescence microscopy and Western blotting) was increased when PECAM-1 or αVβ3 integrin, but not ICAM-1, was used as substrate.</p> <p>Conclusions</p> <p>Direct contact between trophoblasts and endothelial cells increases the expression of trophoblast β1 integrin.</p>http://www.biosignaling.com/content/2/1/4 |
| spellingShingle | Thirkill Twanda L Hendren Sonia R Soghomonians Arlen Mariano Natalie F Barakat Abdul I Douglas Gordon C Regulation of trophoblast beta1-integrin expression by contact with endothelial cells Cell Communication and Signaling |
| title | Regulation of trophoblast beta1-integrin expression by contact with endothelial cells |
| title_full | Regulation of trophoblast beta1-integrin expression by contact with endothelial cells |
| title_fullStr | Regulation of trophoblast beta1-integrin expression by contact with endothelial cells |
| title_full_unstemmed | Regulation of trophoblast beta1-integrin expression by contact with endothelial cells |
| title_short | Regulation of trophoblast beta1-integrin expression by contact with endothelial cells |
| title_sort | regulation of trophoblast beta1 integrin expression by contact with endothelial cells |
| url | http://www.biosignaling.com/content/2/1/4 |
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