Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens
<p>Abstract</p> <p>Background</p> <p>The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken <it>Calpain3 </it>(<it>CAPN3</it>) gene and to analyze the potential association between <it>CAPN3 </it>gene poly...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-03-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/10/10 |
| _version_ | 1818229822081466368 |
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| author | Du Hua-Rui Jiang Xiao-Song Yao Yong-Gang Liu Yi-Ping Zhang Zeng-Rong Zhu Qing |
| author_facet | Du Hua-Rui Jiang Xiao-Song Yao Yong-Gang Liu Yi-Ping Zhang Zeng-Rong Zhu Qing |
| author_sort | Du Hua-Rui |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken <it>Calpain3 </it>(<it>CAPN3</it>) gene and to analyze the potential association between <it>CAPN3 </it>gene polymorphisms and carcass traits in chickens. We screened <it>CAPN3 </it>single nucleotide polymorphisms (SNP) in 307 meat-type quality chicken from 5 commercial pure lines (S01, S02, S03, S05, and D99) and 4 native breeds from Guangdong Province (Huiyang Huxu chicken and Qingyuan Ma chicken) and Sichuan Province (Caoke chicken and Shandi Black-bone chicken), China.</p> <p>Results</p> <p>Two SNPs (11818T>A and 12814T>G) were detected by single strand conformation polymorphism (SSCP) method and were verified by DNA sequencing. Association analysis showed that the 12814T>G genotypes were significantly associated with body weight (BW), carcass weight (CW), breast muscle weight (BMW), and leg muscle weight (LMW). Haplotypes constructed on the two SNPs (H1, TG; H2, TT; H3, AG; and H4, AT) were associated with BW, CW (<it>P </it>< 0.05), eviscerated percentage (EP), semi-eviscerated percentage (SEP), breast muscle percentage (BMP), and leg muscle percentage (LMP) (<it>P </it>< 0.01). Diplotype H1H2 was dominant for BW, CW, and LMP, and H2H2 was dominant for EP, SEP, and BMP.</p> <p>Conclusion</p> <p>We speculated that the <it>CAPN3 </it>gene was a major gene affecting chicken muscle growth and carcass traits or it was linked with the major gene(s). Diplotypes H1H2 and H2H2 might be advantageous for carcass traits.</p> |
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| format | Article |
| id | doaj.art-ed1f57108ec54595a707991170666228 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T10:24:42Z |
| publishDate | 2009-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-ed1f57108ec54595a7079911706662282022-12-22T00:27:28ZengBMCBMC Genetics1471-21562009-03-011011010.1186/1471-2156-10-10Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickensDu Hua-RuiJiang Xiao-SongYao Yong-GangLiu Yi-PingZhang Zeng-RongZhu Qing<p>Abstract</p> <p>Background</p> <p>The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken <it>Calpain3 </it>(<it>CAPN3</it>) gene and to analyze the potential association between <it>CAPN3 </it>gene polymorphisms and carcass traits in chickens. We screened <it>CAPN3 </it>single nucleotide polymorphisms (SNP) in 307 meat-type quality chicken from 5 commercial pure lines (S01, S02, S03, S05, and D99) and 4 native breeds from Guangdong Province (Huiyang Huxu chicken and Qingyuan Ma chicken) and Sichuan Province (Caoke chicken and Shandi Black-bone chicken), China.</p> <p>Results</p> <p>Two SNPs (11818T>A and 12814T>G) were detected by single strand conformation polymorphism (SSCP) method and were verified by DNA sequencing. Association analysis showed that the 12814T>G genotypes were significantly associated with body weight (BW), carcass weight (CW), breast muscle weight (BMW), and leg muscle weight (LMW). Haplotypes constructed on the two SNPs (H1, TG; H2, TT; H3, AG; and H4, AT) were associated with BW, CW (<it>P </it>< 0.05), eviscerated percentage (EP), semi-eviscerated percentage (SEP), breast muscle percentage (BMP), and leg muscle percentage (LMP) (<it>P </it>< 0.01). Diplotype H1H2 was dominant for BW, CW, and LMP, and H2H2 was dominant for EP, SEP, and BMP.</p> <p>Conclusion</p> <p>We speculated that the <it>CAPN3 </it>gene was a major gene affecting chicken muscle growth and carcass traits or it was linked with the major gene(s). Diplotypes H1H2 and H2H2 might be advantageous for carcass traits.</p>http://www.biomedcentral.com/1471-2156/10/10 |
| spellingShingle | Du Hua-Rui Jiang Xiao-Song Yao Yong-Gang Liu Yi-Ping Zhang Zeng-Rong Zhu Qing Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens BMC Genetics |
| title | Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens |
| title_full | Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens |
| title_fullStr | Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens |
| title_full_unstemmed | Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens |
| title_short | Identification and association of the single nucleotide polymorphisms in calpain3 (<it>CAPN3</it>) gene with carcass traits in chickens |
| title_sort | identification and association of the single nucleotide polymorphisms in calpain3 it capn3 it gene with carcass traits in chickens |
| url | http://www.biomedcentral.com/1471-2156/10/10 |
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