Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies
Objectives: Serological assays for the presence of anti-SARS-CoV-2 antibodies are crucially needed for research and monitoring of the SARS-CoV-2 pandemic. Antibodies are reliability detected in capillary blood, a minimally invasive and cost-effective alternative to venous blood testing. However, the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551722000294 |
| _version_ | 1811343177961963520 |
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| author | Stéphanie Baggio Giuseppe Togni Isabella Eckerle Nicolas Vuillemier Laurent Kaiser Laurent Gétaz |
| author_facet | Stéphanie Baggio Giuseppe Togni Isabella Eckerle Nicolas Vuillemier Laurent Kaiser Laurent Gétaz |
| author_sort | Stéphanie Baggio |
| collection | DOAJ |
| description | Objectives: Serological assays for the presence of anti-SARS-CoV-2 antibodies are crucially needed for research and monitoring of the SARS-CoV-2 pandemic. Antibodies are reliability detected in capillary blood, a minimally invasive and cost-effective alternative to venous blood testing. However, there is a limited knowledge on feasibility of capillary blood self-sampling. This study compared the feasibility of capillary blood self-testing in people aged less than 65 vs. people aged 65 or more. A secondary aim was to investigate the performance of the Hem-Col® (no additive) device compared to venous blood testing. Design and methods: Data were collected in a prospective study in Switzerland (n = 106). Capillary blood was collected using the Hem-Col® (no additive) device. Feasibility was assessed using 1) collecting the recommended amount of capillary blood and 2) achieving all steps of capillary blood collection. A sample of 5 ml of venous blood was also collected. Results: For the primary objective, 86.2%/62.1% of patients aged less than 65 collected the recommended amount of capillary blood/achieved all steps vs. 62.5%/39.6% of patients aged 65 or more (p = .006/p = .022). For the secondary objective, the correlation between capillary and venous blood was r = 0.992 and kappa = 1. Conclusions: Capillary blood self-testing appeared as a feasible and reliable alternative to venous blood testing. Such alternative would improve access to serological testing and spare health care resources. However, the difference between age groups should be considered when using self-sampling devices. Help should be developed for older people, such as phone counseling or encouraging asking younger family members for help. |
| first_indexed | 2024-04-13T19:24:00Z |
| format | Article |
| id | doaj.art-ed2082f94a484e008633b9ebb3e84612 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-04-13T19:24:00Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-ed2082f94a484e008633b9ebb3e846122022-12-22T02:33:25ZengElsevierPractical Laboratory Medicine2352-55172022-08-0131e00290Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodiesStéphanie Baggio0Giuseppe Togni1Isabella Eckerle2Nicolas Vuillemier3Laurent Kaiser4Laurent Gétaz5Division of Prison Health, Geneva University Hospitals and University of Geneva, Geneva, Switzerland; Institute of Primary Health Care (BIHAM), University of Bern, Bern, Switzerland; Corresponding author. Division of Prison Health, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.Microbiology Lab, Unilabs, Coppet, SwitzerlandDepartment of Molecular Medicine and Microbiology, Faculty of Medicine, University of Geneva, Geneva, Switzerland; Center for Emerging Viral Diseases, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland; Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland; Laboratory of Virology, Division of Laboratory Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, SwitzerlandDivision of Laboratory Medicine, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland; Division of Laboratory Medicine, Department of Medical Specialties, Geneva University Hospitals and Faculty of Medicine, Geneva, SwitzerlandCenter for Emerging Viral Diseases, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland; Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland; Department of Medicine, Faculty of Medicine, University of Geneva, SwitzerlandDivision of Prison Health, Geneva University Hospitals and University of Geneva, Geneva, Switzerland; Division of Tropical and Humanitarian Medicine, Geneva University Hospitals, Geneva, SwitzerlandObjectives: Serological assays for the presence of anti-SARS-CoV-2 antibodies are crucially needed for research and monitoring of the SARS-CoV-2 pandemic. Antibodies are reliability detected in capillary blood, a minimally invasive and cost-effective alternative to venous blood testing. However, there is a limited knowledge on feasibility of capillary blood self-sampling. This study compared the feasibility of capillary blood self-testing in people aged less than 65 vs. people aged 65 or more. A secondary aim was to investigate the performance of the Hem-Col® (no additive) device compared to venous blood testing. Design and methods: Data were collected in a prospective study in Switzerland (n = 106). Capillary blood was collected using the Hem-Col® (no additive) device. Feasibility was assessed using 1) collecting the recommended amount of capillary blood and 2) achieving all steps of capillary blood collection. A sample of 5 ml of venous blood was also collected. Results: For the primary objective, 86.2%/62.1% of patients aged less than 65 collected the recommended amount of capillary blood/achieved all steps vs. 62.5%/39.6% of patients aged 65 or more (p = .006/p = .022). For the secondary objective, the correlation between capillary and venous blood was r = 0.992 and kappa = 1. Conclusions: Capillary blood self-testing appeared as a feasible and reliable alternative to venous blood testing. Such alternative would improve access to serological testing and spare health care resources. However, the difference between age groups should be considered when using self-sampling devices. Help should be developed for older people, such as phone counseling or encouraging asking younger family members for help.http://www.sciencedirect.com/science/article/pii/S2352551722000294AntibodiesCOVID-19SARS-CoV-2Serological testing |
| spellingShingle | Stéphanie Baggio Giuseppe Togni Isabella Eckerle Nicolas Vuillemier Laurent Kaiser Laurent Gétaz Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies Practical Laboratory Medicine Antibodies COVID-19 SARS-CoV-2 Serological testing |
| title | Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies |
| title_full | Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies |
| title_fullStr | Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies |
| title_full_unstemmed | Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies |
| title_short | Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies |
| title_sort | feasibility of home based elisa capillary blood self testing for anti sars cov 2 antibodies |
| topic | Antibodies COVID-19 SARS-CoV-2 Serological testing |
| url | http://www.sciencedirect.com/science/article/pii/S2352551722000294 |
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