Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain
The aim of this study was to examine the effects of intrastriatally injected nitric oxide synthase (NOS) inhibitors, Nω-nitro-l-arginine methyl ester (L-NAME) and 7-nitroindazole (7-NI), on quinolinic acid (QA)-induced toxicity in selective vulnerable brain regions of adult Wistar rats. QA was a...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2013-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302519S.pdf |
| _version_ | 1819113180116287488 |
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| author | Stevanović Ivana Jovanović Marina Ninković Milica |
| author_facet | Stevanović Ivana Jovanović Marina Ninković Milica |
| author_sort | Stevanović Ivana |
| collection | DOAJ |
| description | The aim of this study was to examine the effects of intrastriatally injected nitric oxide synthase (NOS) inhibitors, Nω-nitro-l-arginine methyl ester (L-NAME) and 7-nitroindazole (7-NI), on quinolinic acid (QA)-induced toxicity in selective vulnerable brain regions of adult Wistar rats. QA was administered into the striatum unilaterally, in a single dose of 150 nM/L with a stereotaxic instrument. The other two experimental groups were pretreated with L-NAME and 7-NI, respectively. The control group of animals was treated with 0.154 mM/L saline solution. The animals were decapitated seven days after the treatment. Samples of both striatum and forebrain cortex were prepared for measurement of acetylcholinesterase (AChE) activity. QA injection revealed a significant increase in AChE activity in both the ipsi- and contralateral striatum and forebrain cortex compared to the control animals. Treatment with NOS inhibitors, followed by QA, very clearly demonstrated lower levels of AChE bilaterally in these brain structures, compared to the QA-treated group. |
| first_indexed | 2024-12-22T04:25:18Z |
| format | Article |
| id | doaj.art-ed20d9aa0004455a9bcde56cf4e14182 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-22T04:25:18Z |
| publishDate | 2013-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-ed20d9aa0004455a9bcde56cf4e141822022-12-21T18:39:10ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392013-01-0165251952410.2298/ABS1302519SNitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brainStevanović IvanaJovanović MarinaNinković MilicaThe aim of this study was to examine the effects of intrastriatally injected nitric oxide synthase (NOS) inhibitors, Nω-nitro-l-arginine methyl ester (L-NAME) and 7-nitroindazole (7-NI), on quinolinic acid (QA)-induced toxicity in selective vulnerable brain regions of adult Wistar rats. QA was administered into the striatum unilaterally, in a single dose of 150 nM/L with a stereotaxic instrument. The other two experimental groups were pretreated with L-NAME and 7-NI, respectively. The control group of animals was treated with 0.154 mM/L saline solution. The animals were decapitated seven days after the treatment. Samples of both striatum and forebrain cortex were prepared for measurement of acetylcholinesterase (AChE) activity. QA injection revealed a significant increase in AChE activity in both the ipsi- and contralateral striatum and forebrain cortex compared to the control animals. Treatment with NOS inhibitors, followed by QA, very clearly demonstrated lower levels of AChE bilaterally in these brain structures, compared to the QA-treated group.http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302519S.pdfAcetylcholinesteraseHuntington’s diseasequinolinic acidNω-nitro-l-arginine methyl ester7-nitroindazole |
| spellingShingle | Stevanović Ivana Jovanović Marina Ninković Milica Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain Archives of Biological Sciences Acetylcholinesterase Huntington’s disease quinolinic acid Nω-nitro-l-arginine methyl ester 7-nitroindazole |
| title | Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| title_full | Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| title_fullStr | Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| title_full_unstemmed | Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| title_short | Nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| title_sort | nitric oxide synthase inhibitors protect cholinergic neurons against quinolinic acid toxicity in the rat brain |
| topic | Acetylcholinesterase Huntington’s disease quinolinic acid Nω-nitro-l-arginine methyl ester 7-nitroindazole |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302519S.pdf |
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